In order to estimate the bitterness intensity of citrus products we applied an adaptative sensory evaluation method, using naringin as the reference for bitterness. Twenty-five untrained subjects participated in this study. Firstly they tasted and positioned 2 naringin references on an unstructured relative-to-reference scaling to define their own bitterness scales. They then evaluated the bitterness intensity of 2 types of bitter orange products (3 distillates and 2 cold-pressed essential oils of bitter orange peels) according to their own perceptions on their own scales. We observed that 2 types of scale use could be distinguished that might be related to subjects’ sensitivity or/and consumption habits to bitterness. As a result, we observed a significant difference in bitterness intensity between the crude cold-pressed essential oil and the debittered one of bitter orange, whereas there was no significant difference between the 3 distillates. This approach makes it possible to take inter-individual differences in subjects’ sensitivity into account. It also provides a way to very simply rate bitterness intensity with subjects who have received no prior training in sensory evaluation.
F. Gutierrez, M. A. Albi, R. Palma, J. J. Rios and J. M. Olias, “Bitter Taste of Virgin Olive Oil: Correlation of Sensory Evaluation and Instrumental HPLC Analysis,” Journal of Food Science, Vol. 54, No. 1, 1989, pp. 68-70. doi:10.1111/j.1365-2621.1989.tb08569.x
F. Gutierrez-Rosales, S. Perdiguero, R. Guterriez and J. M. Olias, “Evaluation of the Bitter Taste in Virgin Olive Oil,” Journal of the American Oil Chemists’ Society, Vol. 69, No. 4, 1992, p. 394.
F. Gutierrez-Rosales, J. J. Rios and M. A. L. Gomez-Rey, “Main Polyphenols in the Bitter Taste of Virgin Olive Oil. Structural Confirmation by on-Line Highperformance Liquid Chromatography Electrospray Ionisation Mass Spec- trometry,” Journal of Agricultural and Food Chemistry, Vol. 51, No. 20, 2003, pp. 6021-6025. doi:10.1021/jf021199x
ISO, “ISO 4121 Analyse Sensorielle. Lignes Directives Pour L’utilisation D’échelles de Réponses Quantitatives,” In: AFNOR Ed., AFNOR, Paris, 2004.
M. Esti, M. Contini, E. Moneta and F. Sinesio, “Phenolics Compounds and Temporal Perception of Bitterness and Pungency in Extra-Virgin Olive Oils: Changes Occurring Throughout Storage,” Food Chemistry, Vol. 113, No. 4, 2009, pp. 1095-1100. doi:10.1016/j.foodchem.2008.08.076
F. Sinesio, E. Moneta and M. Esti, “The Dynamic Sensory Evaluation of Bitterness and Pungency in Virgin Olive Oil,” Food Quality and Preference, Vol. 16, No. 6, 2005, pp. 557-564. doi:10.1016/j.foodqual.2004.12.002
M. O’Mahony, M. Goldenberg, J. Stedmon and J. Alford, “Confusion in the Use of the Taste Adjectives ‘Sour’ and ‘Bitter’,” Chemical Senses & Flavour, Vol. 4, No. 4, 1979, pp. 301-318.
SO, “ISO 8586-1 Analyse Sensorielle. Guide General Pour L’election, L’entrainement et le Controle des Sujets,” In: AFNOR Ed., AFNOR, 1991.
A. H. Pripp, J. Busch and R. Vreeker, “Effect of Viscosity, Sodium Caseinate and Oil on Bitterness Preception of Olive Oil Phenolics,” Food Quality and Preference, Vol. 15, 2004, pp. 375-382. doi:10.1016/S0950-3293(03)00084-3
H. Valentova and J. Pokorny, “Effect of Edible Oils and Oil Emulsions on the Perception of Basic Tastes,” Nahrung, Vol. 42, No. 6, 1998, pp. 406-408. doi:10.1002/(SICI)1521-3803(199812)42:06 3.3.CO;2-Q
G. Haseleu, D. Intelmann and T. Hofmann, “Structure Determination and Sensory Evaluation of Novel Bitter Compounds Formed from B-Acids of Hop (Humulus lupulus L.) upon Wort Boiling,” Food Chemistry, Vol. 116, No. 1, 2009, pp. 71-81. doi:10.1016/j.foodchem.2009.02.008
G. D. Manners, “Citrus Limonoids: Analysis, Bioactivity, and Biomedical Prospects,” Journal of Agricultural and Food Chemistry, Vol. 55, No. 21, 2007, pp. 8285-8294. doi:10.1021/jf071797h
H. S. Lee, “HPLC Analysis of Phenolic Compounds,” In: L. M. L. Nollet, Ed., Food Analysis by HPLC Second edition Revised and Expanded, Marcel Dekker, Inc., Ghent, 2000, pp. 775-824.
P. E. Shaw, “Fruits II,” In: H. Maarse, Ed., Volatile Compounds in Foods and Beverages, Dekker Marcel, Inc., New York, 1991, pp. 305-312, 323-327.
S. Ranganna, V. S. Govindarajan and K. V. R. Ramana, “Citrus Fruits: Part II. Chemistry, Technology, and Quality Evaluation. B. Technology,” Critical Reviews in Food Science and Nutrition, Vol. 18, No 4, 1983, pp. 17-21, 63-65.
J. B. S. Braveman, “II. The Epicarp or Flavedo D. Essential oils, III the Mesocarp or Albedo,” Citrus: Chemical Composition and Chemical Technology Products, Interscience Publishers, Inc, New York, 1949, pp. 40-102.
T. Soldo and T. Hofmann, “Application of Hydrophilic Interaction Liquid Chromatography/Comparative Taste Dilution Analysis for Identification of a Bitter Inhibitor by a Combinatorial Approach Based on Maillard Reaction Chemistry,” Journal of Agricultural and Food Che- mistry, Vol. 53, No. 23, 2005, pp. 9165-9171. doi:10.1021/jf051701o
N. L. Stoer and H. T. Lawless, “Comparison of Single Product Scaling and Relative-to-Reference Scaling in Sensory Evaluation of Dairy Products,” Journal of Sensory Studies, Vol. 8, No. 4, 1992, pp. 257-270. doi:10.1111/j.1745-459X.1993.tb00218.x
N. Larson-Powers and R. M. Pangborn, “Descriptive Analysis of the Sensory Properties of Beverages and Gelatins Containing Sucrose or Synthetic Sweeteners,” Journal of Food Science, Vol. 43, No. 1, 1978, pp. 47-51. doi:10.1111/j.1365-2621.1978.tb09733.x
E. Liden, A. Ulander, F. Deniz and A. G. Gunnarsson, “Odor-Annoyance Estimates from Roadtraffic Combustion Exhausts: Calibration with Master Scaling Using Pyridine as a Reference,” Environment International, Vol. 23, No. 6, 1997, pp. 829-837. doi:10.1016/S0160-4120(97)00095-0
M. C. Meilgaard, G. V. Civille and B. T. Carr, “Sensory Evaluation Techniques,” 3rd Edition, CRC Press LLC, Florida, 1999. doi:10.1201/9781439832271
S. Tanimura and R. D. Mattes, “Relationships between Bitter Taste Sensitivity and Consumption of Bitter Substances,” Journal of Sensory Studies, Vol. 8, No. 1, 1993, pp. 31-41. doi:10.1111/j.1745-459X.1993.tb00200.x
R. D. Mattes, “Influences on Acceptance of Bitter Foods and Beverages,” Physiology & Behavior, Vol. 56, No. 6, 1994, pp. 1229-1236. doi:10.1016/0031-9384(94)90370-0
G. Nota, D. Naviglio, R. Romano, V. Sabia, G. Attanasio and S. S. Musso, “Examination of the Lemon Peel Maceration Step in the Preparation of Lemon Liquor,” Industrie Delle Bevande, Vol. 159, No. 159, 1999, pp. 1-5.
M. L. Crupi, R. Costa, P. Dugo, G. Dugo and L. Mondello, “A Comprehensive Study on the Chemical Composition and Aromatic Characteristics of Lemon Liquor,” Food Chemistry, Vol. 105, No. 2, 2007, pp. 771-783. doi:10.1016/j.foodchem.2007.01.041
H. Vuorela, J. Pohjola, C. Krause and R. Hiltunen, “Application of Headspace Gas Chromatography in Essential Oil Analysis. Part IX. Selective Loss of Terpene Compounds during Hydrodistillation,” Flavour and Fragrance Journal, Vol. 4, No. 3, 1989, pp. 117-120. doi:10.1002/ffj.2730040307
E. Guenther, “Essential Oils of the Genus Citrus,” In: E. Guenther, Ed., The Essentials Oils of the Plant Families Rutaceae and Labiatae, Vol. 3, Van Nostrand Reinhold, New York, 1949, pp. 190-203, 359.
A. G. H. Lea and G. M. Arnold, “The Phenolics of Ciders: Bitterness and Astringency,” Journal of Agricultural and Food Chemistry, Vol. 29, No. 5, 1978, pp. 478-483. doi:10.1002/jsfa.2740290512
Z. Panovska, A. Sediva, M. Jedelska and J. Pokorny, “Effect of Ethanol on Interactions of Bitter and Sweet Tastes in Aqueous Solutions,” Czech Journal of Food Sciences, Vol. 26, No. 2, 2008, pp. 139-145.
J. Lin and I. Blank, “Odorants Generated by Thermally Induced Degradation of Phospholipids,” Journal of Agricultural and Food Chemistry, Vol. 51, No. 15, 2003, pp. 4364-4369. doi:10.1021/jf034300m
W. S. Cain, J. F. Gent, R. B. Goodspeed and G. Leonard, “Evaluation of Olfactory Dysfunction in the Connecticut Chemosensory Clinical Research Center,” Laryngoscope, Vol. 98, 1988, pp. 83-88. doi:10.1288/00005537-198801000-00017
B. Oberti, “Les Facteurs Chimiosensoriels Dans le Comportement Alimentaire des Tunisois,” Montpellier II, Montpellier, 1998.
J. P. Lehrner, J. Gluck and M. Laska, “Odor Identification, Consistency of Label Use, Olfactory Threshold and Their Relationships to Odor Memory over the Human Lifespan,” Chemical Senses, Vol. 24, No. 3, 1999, pp. 337-346. doi:10.1093/chemse/24.3.337
S. S. Schiffman, B. J. Booth, M. L. Losee, S. D. Pecore and Z. S. Warwick, “Bitterness of Sweeteners as a Function of Concentration,” Brain Research Bulletin, Vol. 36, No. 5, 1995, pp. 505-513. doi:10.1016/0361-9230(94)00225-P
L. Brühl, B. Matth?us, E. Fehling, B. Wiege, B. Lehmann, H. Luftmann, K. Bergander, K. Quiroga, A. Scheipers, O. Frank and T. Hofmann, “Identification of Bitter off-Taste Compounds in the Stored Cold Pressed Linseed Oil,” Journal of Agricultural and Food Chemistry, Vol. 55, No. 19, 2007, pp. 7864-7968. doi:10.1021/jf071136k
L. Brühl, B. Matth?us, A. Scheipers and T. Hofmann, “Bitter off-Taste in Stored Cold-Pressed Linseed Oil Obtained from Different Varieties,” European Journal of Lipid Science and Technology, Vol. 110, No. 7, 2008, pp. 625-631. doi:10.1002/ejlt.200700314
J. A. Garcia-Mesa, G. Pereira-Caro, A. Fernandez-Hernandez, C. G.-O. Civantos and R. Mateos, “Influence of Lipid Matrix in the Bitterness Perception of Virgin Olive Oil,” Food Quality and Preference, Vol. 19, No. 4, 2008, pp. 421-430. doi:10.1016/j.foodqual.2007.12.004
K. Nakaya, T. Kohata, N. Doisaki, H. Ushio and T. Ohshima, “Effect of Oil Droplet Sizes of Oil-in-Water Emulsion on the Taste Impressions of Added Tastants,” Fisheries Science, Vol. 72, No. 4, 2006, pp. 877-883. doi:10.1111/j.1444-2906.2006.01230.x
D. McCamey, T. M. Thorpe and J. P. McCarthy, “Bitterness in Foods and Beverages,” In: R. L. Rouseff, Ed., Developments in Food Science, Elsevier Science Publishers, Amsterdam, 1990, pp. 169-182.
L. M. Bartoshuk, “Comparing Sensory Experiences across Individuals: Recent Psychophysical Advances Illuminate Genetic Variation in Taste Perception,” Chemical Senses, Vol. 25, No. 4, 2000, pp. 4647-460. doi:10.1093/chemse/25.4.447
J. X. Guinard, D. Y. Hong and C. Budwig, “Time-Intensity Properties of Sweet and Bitter Stimuli: Implication for Sweet and Bitter Taste Chemoreception,” Journal of Sensory Studies, Vol. 10, No. 1, 1995, pp. 45-71. doi:10.1111/j.1745-459X.1995.tb00004.x
D. R. Redd, T. Tanaka and A. H. McDaniel, “Diverse tastes: Genetics of Sweet and Bitter Perception,” Physiology & Behavior, Vol. 88, No. 3, 2006, pp. 215-226. doi:10.1016/j.physbeh.2006.05.033
J. E. Thurgood and S. Martini, “Effects of Three Emulsion Compositions on Taste Thresholds and Intensity Ratings of Five Taste Compounds,” Journal of Sensory Studies, Vol. 25, No. 6, 2010, pp. 861-875. doi:10.1111/j.1745-459X.2010.00311.x
C. A. McIntosh, R. L. ManSell and R. L. Rouseff, “Distribution of Limonin in the Fruit Tissues of Nine Grapefruit Cultivars,” Journal of Agricultural and Food Chemistry, Vol. 30, No. 4, 1982, pp. 689-692. doi:10.1021/jf00112a016
C. A. McIntosh and R. L. Mansell, “Three-Dimensional Distribution of Limonin, Limonoate A-Ring Monolactone, and Naringin in the Fruit Tissues of Three Varieties of Citrus Paradisi,” Journal of Agricultural and Food Che- mistry, Vol. 45, No. 8, 1997, pp. 2876-2883. doi:10.1021/jf970057d
S. Abbasi, P. Zandi and E. Mirbagheri, “Quantitation of Limonin in Iranian Orange Juice Concentrates Using High-Performance Liquid Chromatography and Spectrophotometric Methods,” European Food Research and Technology, Vol. 221, No. 1-2, 2005, pp. 202-207. doi:10.1007/s00217-005-1136-1
S. Hasegawa and J. E. Hoagland, “Biosynthesis of Limonoids in Citrus,” Phytochemistry, Vol. 16, No. 4, 1977, pp. 469-471. doi:10.1016/S0031-9422(00)94330-9
V. P. Maier and G. D. Beverly, “Limonin Monolactone, the Nonbitter Precursor Responsible for Delayed Bitterness in Certain Citrus Juices,” Journal of Food Science, Vol. 33, No. 5, 1968, pp. 488-492. doi:10.1111/j.1365-2621.1968.tb03661.x