Eugenol, both in its pure form (EG) and included in essential clove oil (CO) was successfully solubilized in aqueous solution by forming inclusion complexes with β -cyclodextrins ( β -CDs) and its modified hydroxy-propyl- β -CDs (HP- β -CDs). To investigate the molecular association between β -CDs/HP- β -CDs with pure EG and essential CO, phase solubility studies were undertaken. Essential CO formed insoluble complexes with β -CDs, but not with HP- β -CDs. The work clearly demonstrates complexes formation follow an order higher than 1:1 when high essential CO and β -CDs concentrations were used, however it was 1:1 in the case of essential CO-HP- β -CDs complexes. When pure EG was studied the results indicated that EG could form 1:1 inclusion complexes with β -CDs and HP- β -CDs. Based on the studies, the Kc values for pure EG were 4555 ± 225 M -1 and 10,633 ± 614 M -1 for β -CDs and HP- β -CDs, respectively, and 2005 ± 199 M -1 for essential CO-HP- β -CDs. These finding indicate that CDs are suitable for encapsulating EG.
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