Effect of FFD-410 (HiOwna-Jr.), a Balanced Herbal Nutritional Supplement on Biochemical, Hematological, Immunological and Cognitive Parameters of Wistar Rats — Oak Academic Publishing
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Effect of FFD-410 (HiOwna-Jr.), a Balanced Herbal Nutritional Supplement on Biochemical, Hematological, Immunological and Cognitive Parameters of Wistar Rats
Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
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Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
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Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
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Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
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Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India
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Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India
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Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
1 Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
2 Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
3 Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
4 Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
5 Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India
6 Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India
7 Department of Pharmacology, R&D Center, The Himalaya Drug Company, Bangalore, India.
Present study was aimed to evaluate the effect HiOwna-Jr. (FFD-410) on vital biochemical, hematological, immunological and cognitive functions in rats; also in vitro antioxidant studies were carried out to evaluate the antioxidant capacity of FFD-410. Animals of respective groups were treatment with FFD-410 for 90 days and blood samples were collected for the estimation of biochemical, hematological parameters and serum immunoglobulin levels; haemoagglutination assay was carried out using Sheep Red blood cells (SRBC’s). In addition, effect of FFD-410 on cognition and memory was evaluated by modified elevated plus maze test. Apart from in vivo studies, in vitro studies such as DPPH radical scavenging assay, reducing power assay and ORAC assays were carried out to evaluate the free radical scavenging and antioxidant activity of FFD-410. Pretreatment with FFD-410 for 90 days did not bring about any change in serum biochemical and hematological parameters and relative organ weights etc., which could account for its wide safety margin at tested dose levels (2.5 and 5.0 g/kg, p.o.). However, FFD-410 showed potent immunostimulant activity by elevating the serum immunoglobulins and haemoagglutination titer values, also the pretreatment with FFD-410 showed dose dependent improvement in short-term cognition and memory in elevated plus maze test. Furthermore, in vitro antioxidant studies FFD-410 exhibited significant and dose dependent free radical scavenging and antioxidant activity as assessed by DPPH (IC50 value of 1162.6 μg/ml), reducing power and ORAC assays (Trolox equivalence/g of 76.1). These findings suggest that, FFD-410 possess very good antioxidant, cognition improving and potent immunostimulant properties. Also, there was no significant change in the serum biochemical and hematological parameters and relative organ weights were observed after 90 days treatment with FFD-410, which could account for its wide safety of margin at tested dose levels (2.5 and5.0 g/kg. p.o.).
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