Heat-Killed <i>Lactobacillus brevis</i> SBC8803 Induces Serotonin Release from Intestinal Cells
- 1 Frontier Laboratories of Value Creation, Sapporo Breweries Ltd., Shizuoka, Japan
- 2 Corporate Planning Department, Sapporo Holdings Ltd., Tokyo, Japan.
- 3 Frontier Laboratories of Value Creation, Sapporo Breweries Ltd., Shizuoka, Japan
Abstract
Previously, we reported that changes induced in autonomic neurotransmission in rats by Lactobacillus brevis SBC8803 may be mediated by serotonin 3 (5-HT3) receptors. In this study, we evaluated the effects of heat-killed L. brevis SBC8803 on serotonin (5-HT) releasing from intestinal cells. In the in vitro study, L. brevis SBC8803 stimulated 5-HT release from cultured rat endocrine RIN-14B cells (SBC8803 vs. sterile water; P < 0.01). For in vivo study, 2 mg of heat-killed L. brevis SBC8803 was administered using a stomach sonde (feeding needle) to C57BL/6J mice. Analysis of plasma by ELISA showed gradually increase in 5-HT concentrations (0 min vs. 60 min; P < 0.05). ELISA of ex vivo cultured intestinal loops composed of duodenum and part of the jejunum, from C3H/HeN and C57BL/6J male mice indicated that L. brevis SBC8803 effectively induced 5-HT release (SBC8803 vs. sterile water; P < 0.01). These experimental results suggest that heat-killed L. brevis SBC8803 may stimulate 5-HT release from mouse intestinal cells such as enterochromaffin cells.
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