Research ArticleOpen AccessGoogle Scholar indexed
Large-Scale Production of Microalgal Lipids Containing High Levels of Docosahexaenoic Acid upon Fermentation of <i>Aurantiochytrium</i> sp. KRS101
Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, South Korea
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan.
Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
- 1 Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
- 2 Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
- 3 Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
- 4 Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
- 5 Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
- 6 Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, South Korea
- 7 Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan.
- 8 Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, South Korea
Food and Nutrition Sciences·Volume 04 (2013)·Pages 1–5·Published 20 August 2013·DOI10.4236/fns.2013.49A1001
Copy link · social · email
Abstract
In this study, large-scale production of microalgal lipid containing high levels of docosahexaenoic acid (DHA) by fermentation of Aurantiochytrium sp. KRS101 was performed. The microalgal strain yielded productivity of docosahex-aenoic acid (DHA) productivity of 1.08 and 1.6 g/L/d by fermentation at 300-L and 5000-L scale stirrer-type bioreactor. The productivity was significantly enhanced upto 5.6 g/L/d by fermentation at 6000-L scale airlift-type bioreactor, probably due to the reduced shearing force. The microalgal lipid could be efficiently recovered by safe extraction methods such as ethanol extraction, hot water extraction or supercritical fluid extraction, promising commercial potential of the microalgal DHA-rich lipid in the food and feed industry.
KeywordsAurantiochytrium sp.Heterotrophic MicroalgaLarge-Scale CultivationLipidDocosahexaenoic Acid
- Y. Li, Z. Zhao and F. Bai, “High-Density Cultivation of Oleaginous Yeast Rhodosporidium toruloides Y4 in Fed-Batch Culture,” Enzyme and Microbial Technology, Vol. 41, No. 3, 2007, pp. 312-317. doi:10.1016/j.enzmictec.2007.02.008
- M. Abbey, P. Clifton, M. Kestin, B. Belling and P. Nestel, “Effect of Fish Oil on Lipoproteins, Lecithin: Cholesterol Acyltransferase, and Lipid Transfer Protein Activity in Humans,” Arteriosclerosis, Vol. 10, No. 1, 1990, pp. 85-94. doi:10.1161/01.ATV.10.1.85
- L. Lauritzen, H. S. Hansen, M. H. Jorgensen and K. F. Michaelsen, “The Essentiality of Long Chain ω-3 Fatty Acids in Relation to Development and Function of the Brain and Retina,” Progress in Lipid Reearchs, Vol. 40, No. 1, 2001, pp. 1-94. doi:10.1016/S0163-7827(00)00017-5
- Y. L. Ma, “Microbial Oils and Its Research Advance,” Chinese Journal of Bioprocess Engineering, Vol. 4, 2006, pp. 7-11.
- Z. Perveen, H. Ando, A. Ueno, Y. Ito, Y. Yamamoto, Y. Yamada, T. Takagi, T. Kaneko, K. Kogame and H. Oku yama, “Isolation and Characterization of a Novel Thraus tochytrid-Like Microorganism that Efficiently Produces Docosahexaenoic Acid,” Biotechnology Letters, Vol. 28, No. 3, 2006, pp. 197-202. doi:10.1007/s10529-005-5335-4
- S. M. Rosa, M. A. Soria, C. G. Velez and M. A. Gal vagno, “Improvement of a Two-Stage Fermentation Proc ess for Docosahexaenoic Acid Production by Aurantio chytrium limacinum SR21 Applying Statistical Experi mental Designs and Data Analysis,” Bioresource Tech nology, Vol. 101, No. 7, 2010, pp. 2367-2374. doi:10.1016/j.biortech.2009.11.056
- L. J. Ren, X. J. Ji, H. Huang, L. Qu, Y. Feng, Q. Q. Tong and R. K. Ouyang, “Development of a Stepwise Aeration Control Strategy for Efficient Docosahexaenoic Acid Production by Schizochytrium sp.,” Applied Microbiology and Biotechnology, Vol. 87, No. 5, 2010, pp. 1649-1656. doi:10.1007/s00253-010-2639-7
- W. K. Hong, D. Rairakhwada, P. S. Seo, S. Y. Park, B. K. Hur, C. H. Kim and J. W. Seo, “Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, Auratiochytrium sp. KRS101,” Applied Microbiology and Biotechnology, Vol. 164, No. 8, 2011, pp. 1468-1480.
- A. M. Burja, H. Radianingtyas, A. Windust and C. J. Barrow, “Isolation and Characterization of Polyunsatu rated Fatty Acid Producing Thraustochytrium Species: Screening of Strains and Optimization of Omega-3 Pro duction,” Applied Microbiology and Biotechnology, Vol. 72, No. 6, 2003, pp. 1161-1169. doi:10.1007/s00253-006-0419-1