High omega-6/omega-3 ratio intake promotes development of many chronic diseases. Secondary prevention studies though have demonstrated a decline in progression of many such diseases after reducing the intake, specific biochemical indices of cardiovascular disease risk markers have not been evaluated. We have evaluated the circulating levels of omega-6/omega-3 ratio and its effect on cardiovascular risk markers in India. Present study was conducted in industrial setting where employees were randomly selected. Data on their demographic characteristics were collected using pre-tested questionnaire. Fasting blood samples were collected from all the participants. Serum was separated and stored at - 80 ℃ till the time of analysis. Lipids were estimated using standard kits. Fatty acids in serum were estimated by Gas chromatography. The identified Omega-3 fatty acid included were 18:3 (Alpha-linolenic acid), 20:5 (Eicosapentenoic acid) & 22:6 (Docosahexenoic acid). Among omega-6 included were 18:2 (linoleic acid), 18:3 (gamma-linolenic acid) & 20:4 (Arachidonic acid). Complete data was available for 176 participants (89% males and 11% females) with mean age of 47.23 ± 6.00 years. The bmi of the participants was 24.88 ±3.43 Kg/m 2 and waist circumference was 91.50 ±9.56 cm. The median of omega-6/omega-3 ratio in the study population was 36.69 (range: 6.21 - 183.69). The levels of total cholesterol, triglycerides, ldl-cholesterol and cholesterol/hdl ratio and apo B correlated significantly with omega-6/3 ratio. There was no correlation observed with hsCRP and LDL-particle size. A direct relationship of omega-6/ omega-3 ratio with dyslipidemia was observed in our study.
A. P. Simopoulos. “The Importance of the Omega-6/ Omega-3 Fatty Acid Ratio in Cardiovascular Disease and Other Chronic Diseases,” Experimental Biology and Medicine, Vol. 233, No. 6, 2008, pp. 674-688. doi:10.3181/0711-MR-311
“WHO Technical Report Series 916. Diet, Nutrition & the Prevention of Chronic Diseases,” WHO, Geneva, 2003.
A. P. Simopoulos and L. G. Cleland, “Omega-6/Omega-3 Essential Fatty Acid Ratio: The Scientific Evidence,” Karger, Basel, 2003.
A. P. Simopoulos, “The Importance of the Ratio of Omega-6/Omega-3 Essential Fatty Acids,” Biomed Pharmacother, Vol. 56, No. 8, 2002, pp. 365-379. doi:10.1016/S0753-3322(02)00253-6
S. A. Udipi, S. A. Karandikar, R. Mukherjee, S. Agarwal and P. S. Ghurge, “Variations in Fats and Fatty Acid Intake in Adult Males of Three Regions of India,” Indian Journal of Public Health, Vol. 50, No. 3, 2006, pp. 179186.
D. Pella, G. Dabnav, R. B. Singh and R. Sharma, “Effects of an Indo-Mediterranean Diet on Omega-6/Omega-3 Ratio in Patients at Risk of CAD: The Indian Paradox,” Karger, Basel, 2003.
Ghafoorunissa, A. Vani, R. Laxmi and B. Sesikeran, “Effects of Dietary Alpha-Linolenic Acid from Blended Oils on Biochemical Indices of Coronary Heart Disease in Indians,” Lipids, Vol. 37, No. 11, 2002, pp. 1077-1086. doi:10.1007/s11745-002-1003-2
B. S. Raheja, S. M. Sadikot, R. B. Pathak and M. B. Rao, “Significance of n-6/n-3 Ratio for Insulin Action in Diabetes,” Annals New York Academy of Sciences, Vol. 683, 1993, pp. 258-271.
Ghafoorunissa, A. Ibrahim, S. Natarajan, “Substituting Dietary Linoleic Acid with Alpha-Linolenic Acid Improves Insulin Sensitivity in Sucrose Fed Rats,” Biochim Biophys Acta, Vol. 1733, No. 1, 2005, pp. 67-75. doi:10.1016/j.bbalip.2004.12.003
Y. Singh, R. Lakshmy, R.Gupta and V. Kranthi, “A Rapid 3% Polyacrylamide Slab Gel Electrophoresis Method for High Throughput Screening for LDL Phenotype,” Lipids in Health & Diseases, Vol. 7, 2008, pp. 47-52. doi:10.1186/1476-511X-7-47
G. Lepage and C. C. Roy, “Direct Transesterification of All Classes of Lipids in a One-Step Reaction,” Journal of Lipid Research, Vol. 27, 1986, pp. 114-120.
T. A. Hague and B. O. Christoffersen, “Evidence for Peroxisomal Retroconversion of Adrenic Acid (22:4n6) and Docosahexaenoic Acid (22:6n3) in Isolated Liver Cells,” Biochim Biophys Acta, Vol. 875, No. 2, 1986, pp. 165173. doi:10.1016/0005-2760(86)90165-7
T. A. Hague and B. O. Christoffersen, “Effect of Dietary Fats on Arachidonic Acid and Eicosapentaenoic Acid Biosynthesis and Conversion to C22 Fatty Acids in Isolated Liver Cells,” Biochim Biophys Acta, Vol. 796, No. 2, 1984, pp. 205-217. doi:10.1016/0005-2760(84)90349-7
E. A. Emken, R. O. Adlof, H. Rakoff and W. K. Rohwedder, “Metabolism of Deuterium-Labeled Linolenic, Linoleic, Oleic, Stearic and Palmitic Acid in Human Subjects,” In: T. A. Baillie and J. R. Jones, Eds., Synthesis and Application of Isotopically Labeled Compounds 1988, Elsevier Science Publishers, Amsterdam, 1989, pp. 713716.
M. Indu and Ghafoorunissa, “n-3 Fatty Acids in Indian Diets—Comparison of the Effects of Precursor (AlphaLinolenic Acid) versus Product (Long Chain n-3 Polyunsaturated Fatty Acids),” Nutrition Research, Vol. 12, No. 4-5, 1992, pp. 569-582. doi:10.1016/S0271-5317(05)80027-2
S. Khandelwal, I. Demonty, P. Jeemon, R. Lakshmy, R. Mukherjee, R. Gupta, U. Snehi, D. Niveditha, Y. Singh, H. C. M. van der Knaap, S. J. Passi, D. Prabhakaran and K. S. Reddy, “Independent and Interactive Effects of Plant Sterols and Fish Oil n-3 Long-Chain Polyunsaturated Fatty Acids on the Plasma Lipid Profile of Mildly Hyperlipidaemic Indian Adults,” British Journal of Nutrition, Vol. 102, No. 5, 2009, pp. 722-732. doi:10.1017/S0007114509297170
K. S. Reddy, D. Prabhakaran, V. Chaturvedi, P. Jeemon, K. R. Thankappan, L. Ramakrishnan, B. V. M. Mohan, C. S. Pandav, F. U. Ahmed, P. P. Joshi, R. Meera, R. B. Amin, R. C. Ahuja, M. S. Das and T. M. Jaison (on Behalf of the Sentinel Surveillance System for Indian Industrial Population Study Group), “Methods for Establishing a Surveillance System for Cardiovascular Diseases in Indian Industrial Population,” Bull World Health Organ, Vol. 84, No. 6, 2006, pp. 461-469. doi:10.2471/BLT.05.027037
A. M. Sawant, D. Shetty, R. Mankeshwar and T. F. Ashavaid, “Prevalence of Dyslipidemia in Young Adult Indian Population,” JAPI, Vol. 56, 2008, pp. 99-102.
H. Petersson, U. Risérus, J. McMonagle, H. L. Gulseth, A. C. Tierney, S. Morange, O. Helal, D. I. Shaw, J. A. Ruano, J. López-Miranda, B. Kiec-Wilk, I. Golabek, E. E. Blaak, W. H. M. Saris, C. A. Drevon, J. A. Lovegrove, H. M. Roche and S. Basu, “Effects of Dietary Fat Modification on Oxidative Stress and Inflammatory Markers in the LIPGENE Study,” British Journal of Nutrition, Vol. 104, No. 9, 2010, pp. 1357-1362. doi:10.1017/S000711451000228X
W. S. Harris, “n-3 Fatty Acids & Serum Lipoproteins: Human Studies,” The American Journal of Clinical Nutrition, Vol. 65, Suppl. 5, 1997, pp. 1645S-1654S.
D. R. Illingworth, W. S. Harris and W. E. Connor, “Inhibition of Low Density Lipoprotein Synthesis by Dietary Omega-3 Fatty Acids in Humans,” Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 4, 1984, pp. 270-275. doi:10.1161/01.ATV.4.3.270
A. J. Parkinson, A. L. Cruz, W. L. Heyward, L. R. Bulkow, D. Hall, L. Barstaed and W. E. Connor, “Elevated Concentrations of Plasma ω-3 Polyunsaturated Fatty Acids among Alaskan Eskimos,” The American Journal of Clinical Nutrition, Vol. 59, 1994, pp. 384-388.
F. B. Hu, L. Bronner, W. C. Willet, M. J. Stampfer, K. M. Rexrode, C. M. Albert, D. Hunter and J. E. Manson, “Fish & Omega-3 Fatty Acid Intake and Risk of CHD in Women,” JAMA, Vol. 287, No. 14, 2002, pp. 1815-1821. doi:10.1001/jama.287.14.1815
Y. Park, and W. S. Harris, “Omega-3 Fatty Acid Supplementation Accelerates Chylomicron Triglyceride Clearance,” Journal of Lipid Research, Vol. 44, 2003, pp. 455463. doi:10.1194/jlr.M200282-JLR200