Effects of Dietary Citric Acid on Metabolic Indicators and Gene Expression in the Skeletal Muscles of Fasted Mice
- 1 Department of Human Sciences and Design, Graduate School of Showa Women’s University, Tokyo, Japan
- 2 Department of Human Health and Design, Showa Women’s University, Tokyo, Japan
Abstract
Citric acid is recognized and utilized as a dietary supplement for its ability to eliminate fatigue. However, the molecular and cellular mechanism underlying this effect has not been elucidated. This study investigated the effect of intragastric citric acid administration on the energy metabolism and gene expression in the skeletal muscles of mice that had been fasted without exercise, using biochemical analyses of metabolic indicators and DNA microarrays. Expression of the genes involved in the glycolysis, tricarboxylic acid cycle and β-oxidation of fatty acids were not affected by citric acid administration. However, citric acid increased the level of blood glucose and expression of the phosphoenolpyruvate carboxykinase gene. These results indicate that citric acid ingestion may eliminate fatigue by promoting gluconeogenesis.
- O. Kajimoto, H. Saigusa, M. Hiramitsu, K. Sakaida and T. Yasuda, “The Internet Investigation about the Attenuation of Fatigue Feeling by Taking Containing Lemon Citric Acid,” Japan Society of Clinical Trials and Research, Vol. 35, No. 7, 2007, pp. 821-828.
- C. Hausswirth, A. X. Bigard, R. Lepers, M. Berthelot and C. Y. Guezennec, “Sodium Citrate Ingestion and Muscle Performance in Acute Hypobaric Hypoxia,” European Journal of Applied Physiology and Occupational Physi ology, Vol. 71, No. 4, 1995, pp. 362-368. http://dx.doi.org/10.1007/BF00240418
- V. Oopik, I. Saaremets, L. Medijainen, K. Karelson, T. Janson and S. Timpmann, “Effects of Sodium Citrate In gestion before Exercise on Endurance Performance in Well Trained College Runners,” British Journal of Sports Medicine, Vol. 37, No. 6, 2003, pp. 485-489. http://dx.doi.org/10.1136/bjsm.37.6.485
- Y. Miyake, K. Yamamoto, M. Nagasaki, N. Nakai, T. Murakami and Y. Shimomura, “The Effect of Post-Exer cise Lemon Juice and Citric Acid Ingestion on Blood Lactic Acid Concentration in Humans,” Japan Society of Nutrition and Food Science, Vol. 54, No. 1, 2001, pp. 29-33. http://dx.doi.org/10.4327/jsnfs.54.29
- S. Saitoh, Y. Yoshitake and M. Suzuki, “Enhanced gly cogen repletion in liver and skeletal Muscle with Citrate Orally Fed after Exhaustive Treadmill Running and Swimming,” Journal of Nutritional Science and Vitami nology, Vol. 29, No. 1, 1983, pp. 45-53. http://dx.doi.org/10.3177/jnsv.29.45
- P. J. Pritchard and D. J. W. Lee, “The Effect of Dietary Citrate on Glycolysis in the Intestinal Mucosa and Liver of the Chicken,” International Journal of Biochemistry, Vol. 3, No. 15, 1972, pp. 322-328. http://dx.doi.org/10.1016/0020-711X(72)90044-4
- T. Sugino, S. Aoyagi, T. Shirai, Y. Kajimoto and O. Ka jimoto, “Effects of Citric Acid and L-Carnitine on Physi cal Fatigue,” Journal of Clinical Biochemistry and Nutri tion, Vol. 41, No. 3, 2007, pp. 224-230. http://dx.doi.org/10.3164/jcbn.2007032
- S. Yasukawa, M. Takamatsu, S. Ebisuno, S. Morimoto, T. Yoshida and T. Okawa, “Citric Acid Metabolism in Urolithiasis 1. Urinary Citric Acid Measurement Using Citrate Lyase,” Japanese Urological Association, Vol. 76, No. 12, 1985, pp. 1848-1853.
- S. Yasukawa, S. Ebisuno, S. Morimoto, M. Uehara and T. Okawa, “An Examination of Serum Citric Acid Meas urement Using Citrate Lyase,” Japanese Urological As sociation, Vol. 82, No. 11, 1991, pp. 1748-1753.
- N. B. Ruderman, A. K. Saha, D. Vavvas and L. A. Wit ters, “Malonyl-CoA, Fuel Sensing, and Insulin Resis tance,” American Journal of Physiology, Vol. 276, No. 1, 1999, pp. E1-E18.