This study aimed to discriminate red wines obtained from organic and biodynamic management by means of proton nuclear magnetic resonance ( 1 H-NMR) and metabonomic investigation. Red wines were produced from Sangiovese grapes over the three harvests 2010-2012 from two parcels of a vineyard, managed according to or ganic (ORG) or biodynamic (BIO) protocol, respectively. Every year the vinifications were performed with commercial selected yeasts (CSY) according to the organic protocol. Moreover, in 2012, CSY vinification was flanked by a spontaneous vinification (SPO) carri ed out by microorganisms naturally present on the grapes. To gain information about the effects of production year, vineyard management, vinification protocol and vines site in the field on the wine characteristics, proton nuclear magnetic resonance spectra were registered by means of a spectrometer operating at 600 MHz and multivariate a nalysis w as carried out. Production year and vinification protocol were found to be the factors affecting most the characteristics of wine me tabolome. Vineyard management seemed to have more limited consequences on the wine composition, with effects on some aminoacids (proline, aspartic acid and valine), alcohols and some polyphenols. The latter observation could not be considered as certain, as vines site in the field acted, in this respect, as a con fo unding element .
A. P. Vastola, “Vino Biodinamico: Un’Opportunità Economica ed Etica,” Book of Abstracts of the 31st World Congress of Vine and Wine, 15-20 June 2008, Verona.
D. Nigro, “Green Revolutionaries. West Coast Winegrowers Fight to Save the Environment,” The Wine Spectator, Vol. 32, No. 6, 2007, pp. 56-71.
A. Miceli, C. Negro, L. Tommasi and P. De Leo, “(DOC) Wines and Wines Obtained from Organic Farming,” Journal of Wine Research, Vol. 14, No. 2-3, 2003, pp. 115-120. http://dx.doi.org/10.1080/09571260410001678030
H. K. Yildirim, A. üren and U. Yücel, “Evaluation of Biogenic Amines in Organic and Non-Organic Wines by HPLC OPA Derivatization,” Food Technology and Biotechnology, Vol. 45, No. 1, 2007, pp. 62-68.
L. Yanez, J. Saavedra, C. Martinez, A. Cordova and M. A. Ganga, “Chemometric Analysis for the Detection of Biogenic Amines in Chilean Cabernet Sauvignon Wines: A Comparative Study between Organic and Nonorganic Production,” Journal of Food Science, Vol. 77, No. 8, 2012, pp. T143-T150. http://dx.doi.org/10.1111/j.1750-3841.2012.02796.x
J. Mulero, F. Pardo and P. Zafrilla, “Effect of Principal Polyphenolic Components in Relation to Antioxidant Activity in Conventional and Organic Red Wines during Storage,” European Food Research and Technology, Vol. 229, No. 5, 2009, pp. 807-812. http://dx.doi.org/10.1007/s00217-009-1117-x
J. Mulero, F. Pardo and P. Zafrilla, “Antioxidant Activity and Phenolic Composition of Organic and Conventional Grapes and Wines,” Journal of Food Composition and Analysis, Vol. 23, No. 6, 2010, pp. 569-574. http://dx.doi.org/10.1016/j.jfca.2010.05.001
S. Tinttunen and P. Lehtonen, “Distinguishing Organic Wines from Normal Wines on the Basis of Concentrations of Phenolic Compounds and Spectral Data,” European Food Research and Technology, Vol. 212, No. 3, 2001, pp. 390-394. http://dx.doi.org/10.1007/s002170000265
J. Mulero, P. Zafrilla, J. M. Cayuela, A. Martínez-Cachá and F. Pardo, “Antioxidant Activity and Phenolic Compounds in Organic red Wine Using Different Winemaking Techniques,” Journal of Food Science, Vol. 76, No. 3, 2011, pp. C436-C440. http://dx.doi.org/10.1111/j.1750-3841.2011.02104.x
B. Gordillo, M. I. Lopez-Infante, P. Ramirez-Perez, M. L. Gonzalez-Miret and F. J. Heredia, “Influence of Prefermentative Cold Maceration on the Color and Anthocyanic Copigmentation of Organic Tempranillo Wines Elaborated in a Warm Climate,” Journal of Agricultural and Food Chemistry, Vol. 58, No. 11, 2010, pp. 6797-6803. http://dx.doi.org/10.1021/jf100084x
D. Cozzolino, M. Holdstock, R. G. Dambergs, W. U. Cynkar and P. A. Smith, “Mid Infrared Spectroscopy and Multivariate Analysis: A Tool to Discriminate between Organic and Non-Organic Wines Grown in Australia,” Food Chemistry, Vol. 116, No. 3, 2009, pp. 761-765. http://dx.doi.org/10.1016/j.foodchem.2009.03.022
P. Plahuta and P. Raspor, “Comparison of Hazards: Current vs. GMO Wine,” Food Control, Vol. 18, No. 5, 2007, pp. 492-502. http://dx.doi.org/10.1016/j.foodcont.2005.12.007
A. Tassoni, N. Tango and M. Ferri, “Comparison of Biogenic Amine and Polyphenol Profiles of Grape Berries and Wines Obtained Following Conventional, Organic and Biodynamic Agricultural and Oenological Practices,” Food Chemistry, Vol. 139, No. 1-4, 2013, pp. 405-413. http://dx.doi.org/10.1016/j.foodchem.2013.01.041
R. Guzzon, M. Malacarne and R. Larcher, “Evolution of Yeast Populations during Different Biodynamic Winemaking Processes,” South African Journal of Enology and Viticulture, Vol. 32, No. 2, 2011.
R. M. Callejon, A. Clavijo, P. Ortigueira, A. M. Troncoso, P. Paneque and M. L. Morales, “Volatile and Sensory Profile of Organic Red Wines Produced by Different Selected Autochthonous and Commercial Saccharomyces cerevisiae Strains,” Analytica Chimica Acta, Vol. 660, No. 1-2, 2010, pp. 68-75. http://dx.doi.org/10.1016/j.aca.2009.09.040
S. Mann, A. Ferjani and L. Reissig, “What Matters to Consumers of Organic Wine?” British Food Journal, Vol. 114, No. 2, 2012, pp. 272-284. http://dx.doi.org/10.1016/j.aca.2009.09.040
J. C. Lindon, J. K. Nicholson and E. Holmes, “The Handbook of Metabonomics and Metabolomics,” Access Online via Elsevier, Amsterdam, 2011.
J. Nicholson, J. Lindon and E. Holmes, “‘Metabonomics’: Understanding the Metabolic Responses of Living Systems to Pathophysiological Stimuli via Multivariate Statistical Analysis of Biological NMR Spectroscopic Data,” Xenobiotica, Vol. 29, No. 11, 1999, pp. 1181-1189. http://dx.doi.org/10.1080/004982599238047
I. Bertini, A. Calabro, V. De Carli, C. Luchinat, S. Nepi, B. Porfirio, et al., “The Metabonomic Signature of Celiac Disease,” Journal of Proteome Research, Vol. 8, No. 1, 2009, pp. 170-177. http://dx.doi.org/10.1021/pr800548z
J. L. Ward, J. M. Baker and M. H. Beale, “Recent Applications of NMR Spectroscopy in Plant Metabolomics,” FEBS Journal, Vol. 274, No. 5, 2007, pp. 1126-1131. http://dx.doi.org/10.1111/j.1742-4658.2007.05675.x
E. Lopez-Rituerto, F. Savorani, A. Avenoza, J. S. H. Busto, J. S. M. Peregrina and S. B. Engelsen, “Investigations of La Rioja Terroir for Wine Production Using 1H NMR Metabolomics,” Journal of Agricultural and Food Chemistry, Vol. 60, No. 13, 2012, pp. 3452-3461. http://dx.doi.org/10.1021/jf204361d
G. Picone, B. Mezzetti, E. Babini, F. Capocasa, G. Placucci and F. Capozzi, “Unsupervised Principal Component Analysis of NMR Metabolic Profiles for the Assessment of Substantial Equivalence of Transgenic Grapes (Vitis vinifera),” Journal of Agricultural and Food Chemistry, Vol. 59, No. 17, 2011, pp. 9271-9279. http://dx.doi.org/10.1021/jf2020717
R. Spaccini, P. Mazzei, A. Squartini, M. Giannattasio and A. Piccolo, “Molecular Properties of a Fermented Manure Preparation Used as Field Spray in Biodynamic Agriculture,” Environmental Science and Pollution Research, Vol. 19, No. 9, 2012, pp. 4214-4225. http://dx.doi.org/10.1007/s11356-012-1022-x
M. F. Alum, P. A. Shaw, B. C. Sweatman, B. K. Ubhi, J. N. Haselden and S. C. Connor, “4,4-Dimethyl-4-Silapentane-1-Ammonium Trifluoroacetate (DSA), a Promising Universal Internal Standard for NMR-Based Metabolic Profiling Studies of Biofluids, Including Blood Plasma and Serum,” Metabolomics, Vol. 4, No. 2, 2008, pp. 122-127. http://dx.doi.org/10.1007/s11306-008-0103-9
K. R. Coombes, H. A. Fritsche, C. Clarke, J.-N. Chen, K. A. Baggerly, J. S. Morris, et al., “Quality Control and Peak Finding for Proteomics Data Collected from Nipple Aspirate Fluid by Surface-Enhanced Laser Desorption and Ionization,” Clinical Chemistry, Vol. 49, No. 10, 2003, pp. 1615-1623. http://dx.doi.org/10.1373/49.10.1615
M. K. H. Liland, T. Almy and B.-H. Mevik, “Optimal Choice of Baseline Correction for Multivariate Calibration of Spectra,” Appied Spectroscopy, Vol. 64, No. 9, 2010, pp. 1007-1016. http://dx.doi.org/10.1366/000370210792434350
F. Savorani, G. Tomasi and S. B. Engelsen, “I Coshift: A Versatile Tool for the Rapid Alignment of 1D NMR Spectra,” Journal of Magnetic Resonance, Vol. 202, No. 2, 2010, pp. 190-202. http://dx.doi.org/10.1016/j.jmr.2009.11.012
F. Dieterle, A. Ross, G. Schlotterbeck and H. Senn, “Probabilistic Quotient Normalization as Robust Method to Account for Dilution of Complex Biological Mixtures. Application in 1H NMR Metabonomics,” Analytical Chemistry, Vol. 78, No. 13, 2006, pp. 4281-4290. http://dx.doi.org/10.1021/ac051632c
D. Delmas, A. Lancon, D. Colin, B. Jannin and N. Latruffe, “Resveratrol as a Chemopreventive Agent: A Promising Molecule for Fighting Cancer,” Current Drug Targets, Vol. 7, No. 4, 2006, pp. 423-442. http://dx.doi.org/10.2174/138945006776359331
R. E. King, J. A. Bomser and D. B. Min, “Bioactivity of Resveratrol,” Comprehensive Reviews in Food Science and Food Safety, Vol. 5, No. 3, 2006, pp. 65-70. http://dx.doi.org/10.1111/j.1541-4337.2006.00001.x
E. Melgarejo, J. L. Urdiales, F. Sánchez-Jiménez and M. á. Medina, “Targeting Polyamines and Biogenic Amines by Green Tea Epigallocatechin-3-Gallate,” Amino Acids, Vol. 38, No. 2, 2010, pp. 519-523. http://dx.doi.org/10.1007/s00726-009-0411-z
M. Iriti and F. Faoro, “Plant Defense and Human Nutrition: Phenylpropanoids on the Menu,” Current Topics in Nutraceutical Research, Vol. 2, No. 2, 2004, pp. 47-65.
R. G. V. Bramley and R. P. Hamilton, “Understanding Variability in Winegrape Production Systems,” Australian Journal of Grape and Wine Research, Vol. 10, No. 1, 2004, pp. 32-45.