Protective Effect of <i>Camellia sinensis</i> on Methotrexate-Induced Small Intestinal Mucositis in Mice
- 1 Center of Endocrinology, Metabolism and Nutrition Studies of Brazilian Northeast, Medicine Faculty/Federal University of Ceará, Fortaleza, Brazil
- 2 Department of Physiology and Pharmacology/Medicine Faculty/Federal University of Ceará, Fortaleza, Brazil
- 3 Medicine/Health Sciences Center, University of Fortaleza, Fortaleza, Brazil
- 4 Department of Pathology and Forensic Medicine/ Medicine Faculty/Federal University of Ceará, Fortaleza, Brazil
- 5 Department of Nutrition/Health Sciences Center/Federal University of Rio Grande do Norte, Natal, Brazil.
Abstract
Background: Green tea has been used as a daily beverage for several years. Anti-inflammatory effect of tea has also been depicted in different papers. Therefore we had set forward this study to examine the potential anti- inflammatory activity of green tea in small intestine mucositis experimental models. Aims: Evaluation of anti- inflammatory effects of green tea on mice. Materials and Methods: Green tea decoction (20%) was prepared by soaking 20 g of green tea in 100 ml boiled water separately, soaked for 2 minutes and thereafter filtered. In - flammatory activity was induced using methotrexate (2.75 g/kg/24 h sc ), and a protecting effect of mucositis con - dition was investigated by vitamin E and Camellia sinensis decoction. Study Design: An experimental study was approved by a n Animal Ethical Commitee. Results: Green tea decoction (20%) has shown significant anti-in - flammatory effects (65% and 70%) on methotrexate-induced acute mucositis model. In villous atrophy Green tea decoction (10% and 20%) has shown no protecting action at different intestinal segments. But at intestinal crypt hyperplasia, green tea decoction has shown 65.74%, as compared to mucositis group. An increase of apoptotic bodies were acchieved at MTX group, CS reduced this occurrence. Conclusion: Taken together, our data indi - cate that green tea (20%) has a potential anti-inflammatory compared vitamin E antioxidant action and cor - roborates with the current trend of tea being promoted as “ health drink ” . However more pharmacological and biochemical assays is necessary to elucidate mechanisms.
- M. Verburg, I. B. Renes, H. P. Meijer, J. A. J. M. Taminiau, H. A. Büller, A. W. C. Einerhand and J. Dekker, “Selective Sparing of Goblet Cells and Paneth Cells in the Intestine of Methotrexate-Treated Rats,” American Journal of Physiology Gastrointestinal Liver Physiology, Vol. 279, No. 5, 2000, pp. G1037-G1047.
- J. S. Trier and T. H. Browning, “Morphologic Response of the Mucosa of Human Small Intestine to X-Ray Exposure,” Journal of Clinical Investigation, Vol. 45, No. 2, 1996, pp. 194-204.
- D. M. Keefe, A. G. Cummins, B. M. Dale, D. Kotasek, T. A. Robb and R. E. Sage, “Effect of High-Dose Chemotherapy on Intestinal Permeability In Humans,” Clinical Sciences, Vol. 92, No. 4, 1997, pp. 385-390.
- J. S. Trier, “Morphologic Alterations Induced by Methotrexate in the Mucosa of Human Proximal Intestine,” Gastroenterology, Vol. 4, 1962, pp. 407-423.
- M. Verburg, I. B. Renes, D. J. Van Nispen, et al., “Specific Responses in Rat Small Intestinal Epithelial mRNA Expression and Protein Levels during Chemotherapeutic Damage and Regeneration,” Journal of Histochemistry and Cytochemistry, Vol. 50, No. 11, 2002, pp. 1525-1536. http://dx.doi.org/10.1177/002215540205001113
- M. Bjekjernes and H. Cheng, “Clonal Analysis of Mouse Intestinal Epithelial Progenitors,” Gastroenterology, Vol. 116, No. 1, 1999, pp. 7-14. http://dx.doi.org/10.1016/S0016-5085(99)70222-2
- J. A. J. M. Tamineau, D. G. Gall and J. R. Hamilton, “Response of the Rat Small-Intestine to Methotrexate,” Gut, Vol. 21, No. 6, 1980, pp. 486-492. http://dx.doi.org/10.1136/gut.21.6.486
- I. L. Cameron, J. Munoz, C. J. Barnes and W. E. Hardman, “High Dietary Level of Synthetic Vitamin E on Lipid Peroxidation, Membrane Fatty Acid Composition and Citotoxicity in Breast Xenograft and in Mouse Host Tissue,” Cancer Cell International, Vol. 3, 2003, p. 3.
- S. Subramaniam and C. S. Shyamala Devi, “Vitamin E Protects Intestinal Basolateral Membrane from CMF-Induced Damages in Rat,” Indian Journal of Physiology and Pharmacology, Vol. 39, No. 3, 1995, pp. 263-266.
- R. A. Warden, S. N. Rhiannon, J. L. Francis, P. R. Dunkley and E. V. O’Loughlin, “Vitamin A Deficiency Exarcerbates Methotrexate-Induced Jejunal Injury in Rats,” Journal of Nutrition, Vol. 127, No. 5, 1997, pp. 770-776.
- C. D. Tran, G. S. Howard, P. Coyle, J. C. Philcox, A. M. Rofe and R. N. Butler, “Dietary Supplementation with Zinc and a Growth Factor Extract Derived from Bovine Cheese Whey Improves Methotrexate-Damage Rat Intestine,” American Journal of Clinical Nutrition, Vol. 77, No. 5, 2003, pp. 1296-1303.