Research ArticleOpen AccessGoogle Scholar indexed
Occurrence of <i>Escherichia coli</i> O157 in Retailed-Beef and Related Meat Products in Zaria, Nigeria
Department of Livestock and Pest Control Services, Federal Ministry of Agriculture, Gombe, Nigeria
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Department of Microbiology, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
Department of Microbiology, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
Centre for Biotechnology Research and Training, Ahmadu Bello University, Zaria, Nigeria
- 1 Department of Livestock and Pest Control Services, Federal Ministry of Agriculture, Gombe, Nigeria
- 2 Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
- 3 Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
- 4 Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
- 5 Department of Microbiology, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
- 6 Department of Microbiology, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
- 7 Centre for Biotechnology Research and Training, Ahmadu Bello University, Zaria, Nigeria
Food and Nutrition Sciences·Volume 05 (2014)·Pages 481–487·Published 7 March 2014·DOI10.4236/fns.2014.56057
Copy link · social · email
Abstract
This study evaluated retailed-beef and related meat products for E. coli O157 in Zaria, Nigeria. Sample types included raw meat, “suya” (roasted meat), “balangu” (barbequed meat), “kilishi” (spiced sun dried meat) and “dambu” (shredded fried meat). A total of 182 samples were analyzed for E. coli O157. Isolates were characterized using conventional biochemical methods and Microbact 12E test kit. Susceptibilities of the isolates to 18 commonly used antimicrobial agents were determined by the disk diffusion method. The carriage of stx 1 and stx 2 genes was determined by PCR. Microbact confirmed 4 E. coli isolates. All isolates exhibited multiple drug resistance to the antimicrobial agents tested. An overall prevalence of 2.2% was obtained for E. coli O157. All 4 isolates of E. coli O157 were isolated from raw meat; two of which harboured the stx 1 gene.
Keywords<i>E. coli</i>O157Meat ProductsMultiple Drug ResistanceFood Safety
- Karch, H., Tarr, P. and Bielazewska, M. (2005) Enterohaemorrhagic Escherichia coli in Human Medicine. International Journal of Medical Microbiology, 295, 405-418. http://dx.doi.org/10.1016/j.ijmm.2005.06.009
- Nataro, J.P. and Kaper, J.B. (1998) Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11, 142-201.
- Paton, J.C. and Paton, A.W. (1998) Pathogenesis and Diagnosis of Shigatoxin Producing Escherichia coli Infections. Clinical Microbiology Reviews, 11, 450-479.
- Coia, J.E. (1998) Clinical, Microbiological and Epidemiological Aspects of Escherichia coli O157 Infection. FEMS Immunology and Medical Microbiology, 20, 1-9. http://dx.doi.org/10.1016/S0928-8244(97)00105-3
- Schmidt, H., Beutin, L. and Karch, H. (1995) Molecular Analysis of the Plasmid-Encoded Hemolysin of Escherichia coli O157:H7 Strain EDL933. Infection and Immunity, 63, 1055-1061.
- Beutin, L., Montenegro, M.A., Orskov, I., Orskov, F., Prada, J., Zimmermann, S. and Stephan, R. (1995) Close Association of Verotoxin (Shiga-Like Toxin) Production with Enterohemolysin Production in Strains of Escherichia coli. Journal of Clinical Microbiology, 27, 2259-2264.
- Armstrong, G.L., Hollingsworth, J. and Morris Jr., J.G. (1996) Emerging Food Borne Pathogens: Escherichia coli O157:H7 as a Model of Entry of a New Pathogen into the Food Supply of the Developing World. Epidemiology Reviews, 18, 29-51. http://dx.doi.org/10.1093/oxfordjournals.epirev.a017914
- Mortimore, M.J. (1970) Zaria and Its Region. Annals of the Association of American Geographers, 60, 73-80.
- Barrow, G.I. and Feltham, R.K.A. (1995) Cowan and Steel’s Manual for the Identification of Medical Bacteria. Cambridge University Press, 94-163.
- Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M. (1966) Antibiotic Susceptibility Testing by a Standardized Single Disk Method. American Journal of Clinical Pathology, 45, 493-496.
- Clinical Laboratory Standards Institute (CLSI) (2006) Performance Standards for Antimicrobial Susceptibility Testing. Sixth Informational Supplement CLSI Document M100-516, Wayne.
- Al-Gallas, N., Ben Aissa, R., Attia Annabi, Th., Bahri, O. and Boudabous, A. (2002) Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Meat and Dairy Products. Food Microbiology, 19, 389-398. http://dx.doi.org/10.1006/fmic.2002.0488
- Abd El-Atty, N.S. and Meshref, A.M.S. (2007) Prevalence of Salmonella and E. coli O157 in Some Foods. Beni-Suef Vet. Medicine Journal, 5th. Scientific Conference, 73-78.