1-Deoxynojirimycin Content and Alfa-Glucosidase Inhibitory Activity and Heat Stability of 1-Deoxynojirimycin in Silkworm Powder
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Abstract
Silkworm powder containing 1-deoxynojirimycin (DNJ) has α-glucosidase inhibitory activity and is promising as a complementary and alternative medicine (CAM) agent in Japan. Silkworm powder produced in Korea was extracted with 75% ethanol. The extract was derivatized with 9-fluorenylmethyl chloroformate (FMOC-Cl), and DNJ-FMOC content was measured by HPLC. Then, alfa-glucosidase inhibition by silkworm and mulberry powder in pig liver crude enzyme was assayed using 4-nitrophenyl-alfa-D-glucopyranoside as a substrate. Silkworm powder DNJ content (0.39 to 0.58%) was higher than that in mulberry powder (0.08 to 0.12%). The alfa-glucosidase inhibitory activity of silkworm powder was more potent than that of mulberry leaves and green tea. Silkworm powder DNJ was stable upon heating to 121?C for up to 15 min.
- K.-S. Ryu, H.-S. Lee and I. Kim, “Effects and Mechanisms of Silkworm Powder as a Blood Glucose-Lowering Agent,” International Journal of Industrial Entomology, Vol. 4, 2002, pp. 93-100.
- T. Oku, M. Yamada, M. Nakamura, N. Sadamori and S. Nakamura, “Inhibitory Effects of Extractives from Leaves of Morus Alba on Human and Rat Small Intestinal Disaccharidase Activity,” British Journal of Nutrition, Vol. 95, No. 5, 2006, pp. 933-938. doi:10.1079/BJN20061746
- N. Asano, E. Tomioka, H. Kizu and K. Matsui, “Sugars with Nitrogen in the Ring Isolated from the Leaves of Morus Bombycis,” Carbohydrate Research, Vol. 253, 1994, pp. 235-245. doi:10.1016/0008-6215(94)80068-5
- N. Asano, T. Yamashita, K. Yasuda, K. Ikeda, H. Kizu, Y. Kameda, A. Kato, R.-J. Nash, H.-S. Lee and K.-S. Ryu, “Polyhydroxylated Alkaloids Isolated from Mulberry Trees (Morus Alba l.) and Ailkworms (Bombyx Mori l.),” Journal of Agricultural and Food Chemistry, Vol. 49, 2001, pp. 4208-4213. doi:10.1021/jf010567e
- T. Saito and I. Yamaguchi, “Effect of Glycosylation and Glucose Trimming Inhibitors on the Influenza Avirus Glycoproteins,” Journal of Veterinary Medical Science, Vol. 62, 2000, pp. 575-581. doi:10.1292/jvms.62.575
- Y. Tanaka, J. Kato, M. Kohara and M.-S. Galinski, “Antiviral Effects of Glycosylation and Glucose Trimming Inhibitors on Human Parainfluenza Virus Type 3,” Antiviral Research, Vol. 72, No. 1, 2006, pp. 1-9. doi:10.1016/j.antiviral.2006.03.016
- E. Bause, J. Schweden, A. Gross and B. Orthen, “Purification and Characterization of Trimming Glucosidase I from Pig Liver,” European Journal of Biochemistry, Vol. 183, No. 3, 1989, pp. 661-669. doi:10.1111/j.1432-1033.1989.tb21096.x
- J. Schweden and E. Bause, “Characterization of Trimming Man9-Mannosidase from Pig Liver,” Biochemistry Journal, Vol. 264, No. 2, 1989, pp. 347-355.
- K. Yatsunami, H. Ichida and S. Onodera, “The Relationship between 1-Deoxynojirimycin Content and α-Glucosidase Inhibitory Activity in Leaves of 276 Mulberry Cultivars (Morus Spp.) in Kyoto, Japan,” The Journal of Natural Medicines, Vol. 62, 2008, pp. 63-66. doi:10.1007/s11418-007-0185-0
- T. Anno, K. Tamura, H. Oono and H. Tomi, “Maltase, Sucrase and α-Amylase Inhibitory Activity of Morus Leaves Extract,” Food Preservation Sciences, Vol. 30, No. 5, 2004, pp. 223-229.
- T. Kimura, K. Nakagawa, Y. Saito, K. Yamaguchi, M. Suzuki, K. Yamaki, H. Shinmoto and T. Miyazawa, “Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection,” Journal of Agricultural and Food Chemistry, Vol. 52, 2004, pp. 1415- 1419. doi:10.1021/jf0306901