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Comparative Analysis of the Antioxidant and Free-Radical Scavenging Activities of Different Water-Soluble Extracts of Green, Black and Oolong Tea Samples

¹ Nutrición y Bromatología, Facultad de Medicina and CINAD (Centro de Investigación en Nutrición, Alimentación y Dietética; Edificio Lucia-Parque Científico), Universidad de Valladolid, Valladolid, Spain
² Nutrición y Bromatología, Facultad de Medicina and CINAD (Centro de Investigación en Nutrición, Alimentación y Dietética; Edificio Lucia-Parque Científico), Universidad de Valladolid, Valladolid, Spain
³ Nutrición y Bromatología, Facultad de Medicina and CINAD (Centro de Investigación en Nutrición, Alimentación y Dietética; Edificio Lucia-Parque Científico), Universidad de Valladolid, Valladolid, Spain
⁴ Farmacia y Tecnología Farmacéutica, Facultad de Farmacia and IUFI (Instituto Universitario de Farmacia Industrial), Universidad Complutense de Madrid, Madrid, Spain
⁵ Higiene de los Alimentos y Tecnología, Facultad de Veterinaria, Universidad de León, León, Spain
⁶ Nutrición y Bromatología, Facultad de Medicina and CINAD (Centro de Investigación en Nutrición, Alimentación y Dietética; Edificio Lucia-Parque Científico), Universidad de Valladolid, Valladolid, Spain
⁷ Nutrición y Bromatología, Facultad de Medicina and CINAD (Centro de Investigación en Nutrición, Alimentación y Dietética; Edificio Lucia-Parque Científico), Universidad de Valladolid, Valladolid, Spain
⁸ Nutrición y Bromatología, Facultad de Medicina and CINAD (Centro de Investigación en Nutrición, Alimentación y Dietética; Edificio Lucia-Parque Científico), Universidad de Valladolid, Valladolid, Spain

Food and Nutrition Sciences·Volume 05 (2014)·Pages 2157–2166·Published 1 December 2014·DOI10.4236/fns.2014.522228

Abstract

Extracts of 40 green, black or oolong high quality tea samples from China, Japan or India among others, have been prepared according to the manufacturer instructions and assayed for total polyphenols content using the Folin-Ciocalteu phenol reagent. In addition, the antioxidant activity was assessed by the CUPRAC method and the free-radical scavenging activity was determined using the antioxidant-promoting decay of the stable free-radical DPPH. Caffeine and the most important catechins were identified using RP-HPLC previously validated method. Results from this study suggest that teas, even though they content similar amounts of caffeine, differ considerably in polyphenolic content and therefore in antioxidant and free-radical scavenging activities, depending on the part of the plant used and the process applied to the material for the preparation of the final tea presentation. Correlations among Folin-Ciocalteau’s reactivity of tea samples, the neocuproine reactivity, the DPPH decolourization and the HPLC analysis suggest that the antioxidant activity is due essentially to polyphenolic compounds present in teas, mainly EGCG. The best extraction method of tea powder was the one indicated by dealers. In general, the richest samples belong to green teas and the poorest samples belong to the black teas.

KeywordsTeaPolyphenolsAntioxidantsFree-Radical Scavenging

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Comparative Analysis of the Antioxidant and Free-Radical Scavenging Activities of Different Water-Soluble Extracts of Green, Black and Oolong Tea Samples

Abstract

References (23)