This work aims at analyzing the bioactive and anti-nutritional compounds of edible and wild beans when unprocessed and malted. Qualitative screening of phytochemicals in the various bean samples was determined in ethanol and petroleum ether solvents. Results of the anti-nutritional compositions of unprocessed wild bean extracted with petroleum ether showed there were no traces of saponin and polyphenol, in Feregede and also in edible bean-IT07K-243-1-10 which also had no traces of saponin and tannin. After malting, saponin was totally absent in Pakala , Mucuna , IT97k-499-35, IT07k-243-1-10, and IT04k-333-2 respectively. Polyphenol was also found to be absent in IT07k-243-1-10. Mucuna has the highest phytic acid level (7.8867 ± 0.011) while Feregede has the lowest phytic acid level (2.9810 ± 0.004). Otili has the highest anti-trypsin level (12.001 ± 0.0013). This study showed varying levels of anti-nutrients on the respective bean samples when unprocessed but decreased marginally after malting. It was keenly noted that values derived, either before and after malting were not significantly different (P ≤ 0.05) from each other. In all, this study had further shown that malting process enhanced removal of anti-nutrients which invariably would lead to availability of nutrient for animal and human consumption.
KeywordsBeansSaponinTanninTrypsinPolyphenol
Morris, B. (2003) Legumes. In: Katz, S.H., Ed., Encyclopedia of Food and Culture, Vol. 3, Charles Scribner & Sons, New York, 1-2.
Doyle, J. (1994) Phylogeny of the Legume Family: An Approach to Understanding the Origins of Nodulation. Annual Review of Ecology, Evolution, and Systematics, 25, 325-349. http://dx.doi.org/10.1146/annurev.es.25.110194.001545
Ali, M. and Kumar, S. (2000) Problems and Prospects of Pulses Research in India. Indian Farm, 50, 4-13.
Vucenik, I. and Shamsuddin, A.M. (2006) Protection against Cancer by Dietary IP6 and Inositol. Nutrition and Cancer, 55, 109-125. http://dx.doi.org/10.1207/s15327914nc5502_1
Hangen, L. and Bennink, M.R. (2002) Consumption of Black Beans and Navy Beans (Phaseolus vulgaris) Reduced Azoxymethane-Induced Colon Cancer in Rats. Nutrition and Cancer, 44, 60-65. http://dx.doi.org/10.1207/S15327914NC441_8
Finley, J.W., Burrell, J.B. and Reeves, P.G. (2007) Pinto Bean Consumption Changes SCFA Profiles in Fecal Fermentations, Bacterial Populations of the Lower Bowel and Lipid Profiles of Human. Journal of Nutrition, 137, 2391-2398.
Mitchell, D.C., Lawrence, F.R., Hartman, T.J. and Curran, J.M. (2009) Consumption of Dry Beans, Peas, and Lentils Could Improve Diet Quality in the US Population. Journal of the American Dietetic Association, 109, 909-913. http://dx.doi.org/10.1016/j.jada.2009.02.029
Liener, I.E. (1981) Factors Affecting the Nutritional Quality of Soy Product. Journal of the American Oil Chemists’ Society, 58, 406-415. http://dx.doi.org/10.1007/BF02582390
Chitra, U., Vimala, V., Singh, U. and Geervani, P. (1995) Variability in Phytic Acid Content and Protein Digestibility of Grain Legumes. Plant Foods for Human Nutrition, 47, 163-172. http://dx.doi.org/10.1007/BF01089266
Burbano, C., Muzquiz, M., Ayet, G., Guadrado, C. and Pedrosa, M.M. (1999) Evaluation of Antinutritional Factors of Selected Varieties of Phaseolus vugaris. Journal of the Science of Food and Agriculture, 79, 1468-1472. http://dx.doi.org/10.1002/(SICI)1097-0010(199908)79:11 3.0.CO;2-G
Beecher, G.R. (2003) Overview of Dietary Flavonoids: Nomenclature, Occurrence and Intake. Journal of Nutrition, 133, 3248S-3254S.
Xu, B. and Chang, S.K. (2009) Total Phenolic, Phenolic Acid Anthocyanin, Flavan-3-ol and Flavonol Profiles and Antioxidant Properties of Pinto and Black Beans (Phaseolus vulgaris L) as Affected by Thermal Processing. Journal of Agricultural and Food Chemistry, 57, 4754-4764. http://dx.doi.org/10.1021/jf900695s
Gupta, J.J., Yadav, B.P.S., Gupta, H.K., Sahoo, S.K. and Agrahar, D. (2001) Nutritive Value of Detoxified Jack Bean (Canavalia ensiformis) Seed for Young Chicks. The Indian Journal of Animal Sciences, 71, 1169-1171.
Coulter, J.B., Sulliman, G.I., Omer, M.I., Macfarlene, S.B., Moody, J.B. and Hendrickse, R.G. (1988) Protein Energy Malnutrition in Northern Sudan: Clinical Studies. European Journal of Clinical Nutrition, 42, 787-796.
Chel-Guerrero, L., Perez-Flores, V., Bentacur-Ancona, D. and Davila-Ortiz, G. (2002) Functional Properties of Flours and Protein Isolates from Phaseolus lunatus and Canavalia ensiformis Seeds. Journal of Agricultural and Food Chemistry, 50, 584-591. http://dx.doi.org/10.1021/jf010778j
Arinathan, V., Mohan, V.R. and De-Britto, A.J. (2003) Chemical Composition of Certain Pulses in South India. International Journal of Food Sciences and Nutrition, 54, 209-217. http://dx.doi.org/10.1080/09637480120092026
Rooney, L.W. and Miller, F.R. (1982) Variation in the Structure and Kernel Characteristics of Sorghum. Proceedings of the International Symposium on Sorghum Grain Quality, International Crop Research Institute for the Semi-Arid Tropics, Hyderabad, 28-31 October 1981, 11-13.
IOB (1981) Report of the Analysis Committee of the Institute of Brewing. Institute of Brewing, London, 14-15.
Awoyinka, O.A. and Adebawo, O.O. (2007) Influence of Variety and Malting Period on Alpha and Beta Amylases in Different Nigerian Maize Cultivars. Technical Quarterly—The Master Brewers Association of Americas, 44, 252-255 http://www.tqmbaa.org
Sofowora, A. (1993) Medicinal Plants and Traditional Medicines in Africa. John Wiley & Sons, New York, Chichester, 97-145.
Harborne, J.B. (1973) Photochemical Methods: A Guide to Modern Techniques of Plant Analysis. Chapman A. & Hall, London, 279.
Kapoor, L.D., Singh, A., Kapoort, S.L. and Strivastava, S.N. (1969) Survey of Indian Medicinal Plants for Saponins, Alkaloids and Flavonoids. Lloydia, 32, 297-302.
Earnsworth, N.R., Berderka, J.P. and Moses, M. (1974) Screening of Medicinal Plants. Journal of Pharmaceutical Sciences, 63, 457-459.
Nwosu, J.N. (2013) Evaluation of the Proximate Composition and Antinutritional Properties of African Yam Bean (Sphenostylis sternocarpa) Using Malting Treatment. International Journal of Basic and Applied Sciences, 2, 157-169.
Babar, V.S., Chavan, J.K. and Kadam, S.S. (1988) Effects of Heat Treatments and Germination on Trypsin Inhibitor Activity and Polyphenols in Jack Bean (Canavalia ensiformis L. DC). Plant Foods for Human Nutrition, 38, 319-324. http://dx.doi.org/10.1007/BF01091729
AOAC (1990) Methods of the Association of Official Analysis Chemists. Official Methods of Analysis. 15th Edition, Assoc. Official Analytical Chemists, Virginia, 1141.
Gurumoorthi, P., Pugalenthi, M. and Janardhanan, K. (2003) Nutritional Potential of Five Accessions of a South Indian Tribal Pulse, Mucuna pruriens var. utilis 1. Investigation on the Total Free Phenolics, Tannins, Trypsin and Chymotrypsin Inhibitors, Phytohaemagglutinins and In-Vitro Protein Digestibility. Tropical and Subtropical Agroecosystems, 1, 153-158.
John, S.K.P., George, S., Primrose, J.N. and Fozard, J.B.J. (2011) Symptoms and Signs in Patients with Colorectal Cancer. Colorectal Disease, 13, 17-25.
Asquith, T.N. and Butler, L.G. (1986) Interaction of Condensed Tannins with Selected Proteins. Phytochemistry, 25, 1591-1593. http://dx.doi.org/10.1016/S0031-9422(00)81214-5
Koratkar, R. and Rao, A.V. (1997) Effect of Soybean Saponins on Azoxymethane-Induced Prneoplastic Lesions in the Colon of Mice. Nutrition and Cancer, 27, 206-209. http://dx.doi.org/10.1080/01635589709514526
Price, K.R., Johnson, T.I. and Fenwick, G.R. (1987) The Chemistry and Biological Significance of Saponins in Food and Feeding Stuffs. Critical Reviews in Food Science and Nutrition, 26, 22-48.
Ajayi, I.A., Oderinde, R.A., Kajogbola, D.O. and Ukponi, J.U. (2006) Oil Content and Fatty Acid Composition of Some Underutilized Legumes from Nigeria. Food Chemistry, 99, 115-120. http://dx.doi.org/10.1016/j.foodchem.2005.06.045
Tsao, R. (2010) Chemistry and Biochemistry of Dietary Polyphenol. Nutrients, 2, 1231-1246. http://dx.doi.org/10.3390/nu2121231
De Mejia, E., Guzman-Maldonado, S.H., Acosta-Gallegos, J.A., Reynoso-Camacho, R., Ramirez-Rodriguez, E., Pons-Hernandez, J.L., Gonzalez-Chavira, M.M., Castellanos, J.Z. and Nelly, J.D. (2003) Effect of Cultivar and Growing Location on the Trypsin Inhibitors, Tannins and Lectins of Common Beans (Phaseolus vulgaris L.) Grown in Semiarid Highlands of Mexico. Journal of Agricultural and Food Chemistry, 51, 5962-5966. http://dx.doi.org/10.1021/jf030046m
Hughes, J.S., Ganthavorn, C. and Wilson-Sanders, S. (1997) Dry Beans Inhibit Azixymethane-Induced Colon Carcinogenesis in F344 Rats. Journal of Nutrition, 127, 2328-2333.
Hollman, P.C. (2004) Absorption, Bioavailability and Metabolism of Flavonoids. Pharmaceutical Biology, 42, 74-83. http://dx.doi.org/10.3109/13880200490893492
Bravo, L. (1998) Polyphenol: Chemistry, Dietary Sources, Metabolism and Nutritional Significance. Nutrition Reviews, 56, 317-333. http://dx.doi.org/10.1111/j.1753-4887.1998.tb01670.x
Hagerman, A.E., Riedl, K.M., Jones, G.A., Sovik, K.N., Ritchard, N.T., Hartfield, P.W. and Riechel, T.L. (1998) High Molecular Weight Plant Polyphenolics (Tannins) as Biological Antioxidants. Journal of Agricultural and Food Chemistry, 46, 1887-1892. http://dx.doi.org/10.1021/jf970975b
Cardador-Martinez, A., Loarca-Pina, G. and Oomah, B.D. (2002) Antioxidant Activity in Common Beans (Phaseolus vulgaris L.). Journal of Agricultural and Food Chemistry, 50, 6975-6980. http://dx.doi.org/10.1021/jf020296n
Geil, P.B. and Anderson, J.W. (1994) Nutrition and Health Implications of Dry Beans: A Review. Journal of the American College of Nutrition, 13, 549-558. http://dx.doi.org/10.1080/07315724.1994.10718446
Ferguson, L.R., Chaven, R.R. and Harris, P.J. (2001) Changing Concepts of Dietary Fiber: Implications for Carcinogenesis. Nutrition and Cancer, 39, 155-169. http://dx.doi.org/10.1207/S15327914nc392_1