Clinical, Hematological and Biochemical Health Benefit Effects of <i>Hibiscus sabdariffa</i> Lin Dried Calyces Beverage in Human — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Clinical, Hematological and Biochemical Health Benefit Effects of <i>Hibiscus sabdariffa</i> Lin Dried Calyces Beverage in Human
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
,
Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
,
Penka-Michel’s District Hospital Laboratory, Penka-Michel, Cameroon
,
Espace Priorité Santé du Cameroun Health Center, Dschang, Cameroon
,
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
1 Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
2 Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
3 Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
4 Penka-Michel’s District Hospital Laboratory, Penka-Michel, Cameroon
5 Espace Priorité Santé du Cameroun Health Center, Dschang, Cameroon
6 Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Background/Objective: This study was carried out to investigate the health benefit effects of Hibiscus sabdariffa Lin ( H. sabdariffa L.) dried calyces beverage on some clinical, biochemical and hematological parameters in humans. Methods: The dried calyces were harvested in the two regions (Adamaoua and West) of Cameroon. The proximate, mineral composition and phytochemical screening were evaluated. A standardized extraction procedure was set up and from the calyces; we prepared a drink for 32 male volunteers’ subjects aged from 21 to 32 years, specially recruited for the experiment. Each participant consumed 500 mL twice a day (in the morning and in the evening) as supplement beverage during two weeks. The anthropometrics (age, height, weight, body mass index (BMI)), clinical (systolic and diastolic blood pressure), hematological (RBC, Hb, PCV, MCV, MCH, MCHC, WBC, Lymphocytes, MID cells, Granulocytes, platelet and MPV) and biochemical (TC, HDL-C, LDL-C, TG, serum iron, blood glucose, creatinine, urea, ASAT and ALAT) parameters were determined in the blood on days 0 and at the end of each week. Results: Crude protein, lipid, fiber and ash content of calyx ranged respectively from 4.57 - 5.98, 10.10 - 11.33, 20.39 - 22.30 and 9.15% - 10.38% while the levels of minerals were ranged from 512.0 - 740.6, 77.8 - 177.7, 52.84 - 52.85, 1.10 - 2.10, 41.2 - 119.5, 3.25 - 8.20 and 0.56 - 17.5 mg/100g respectively for Ca, Mg, K, Na, P, Fe and Zn. The phytochemical screening tests revealed the presence of alkaloids, flavonoids, tannins, phenols and anthocyanins on methanol and aqueous extracts. A significant increase of RBC, Hb, PCV, MPV, HDL-C, TG and creatinine and a significant decrease of WBC, MID cells, LDL-C and TC (p < 0.05) were observed during the study period. Furthermore, there was no significant change on BMI, MCV, MCH, MCHC, lymphocyte, granulocyte, platelet, serum iron, blood glucose, ASAT, ALAT and urea levels. Conclusion: H. sabdariffa L. dried calyces from Cameroon are rich sources of crude fibers and minerals. The H. Sabdariffa L. dried calyces drink can be safely used for people suffering for anemia. It also revealed good cholesterol lowering potential. No hepatoxicity and no kidney damage have been observed as far as serum enzymes were concerned.
Kromhout, D., Bloemberg, B.P., Feskens, E.J., Hertog, M.G., Menotti, A. and Blackburn, H. (1996) Alcohol, Fish, Fiber and Antioxidant Vitamins Intake Do Not Explain Population Differences in Coronary Heart Disease Mortality. International Journal of Epidemiology, 25, 753-759. <br/〉http://dx.doi.org/10.1093/ije/25.4.753
ILSI (1999) Safety Assessment and Potential Health Benefits of Food Components Based on Selected Scientific Criteria. ILSI North America Technical Committee on Food Components for Health Promotion. Critical Reviews in Food Science and Nutrition, 39, 203-216. <br/〉http://dx.doi.org/10.1080/10408699991279169
Agea, J.B., Kimondo, J.M., Okia, C.A., Abohassan, R.A., Obua, J., Hall, J. and Teklehaimanot, Z. (2010) Contribution of Wild and Semi Food Plants to Overall House Hold Diet in Bunyoro-Kitara Kingdom, Uganda. Agricultural Journal, 6, 134-144.
Babalola, S.O., Babalola, A.O. and Aworh, O.C. (2000) Compositional Attributes of the Calyces of Roselle (Hibiscus sabdariffa L.). Journal of Food Technology in Africa, 6, 133-134.
Mckay, D.L., Oliver Chen, C.Y., Saltzman, E. and Blumberg, J.B. (2010) Hibiscus sabdariffa L. Tea Lowers Blood Pressure in Pre Hypertensive and Mildly Hypertensive Adults. The Journal of Nutrition, 140, 298-303. <br/〉http://dx.doi.org/10.3945/jn.109.115097
Maffo, T.G., Etamé, L.G., Pankoui, M.J.B., Feukeng, M., Fotso, M.K.H., Tchouanguep, M.F. and Gouado, I. (2011) Effect of Folere Juice (Calyx of Hibiscus sabdariffa Lin) on Some Biochemical Parameters in Humans. Pakistan Journal of Nutrition, 10, 755-759. <br/〉http://dx.doi.org/10.3923/pjn.2011.755.759
Harbone, J.B. (1973) Phytochemical Methods. In: A Guide to Modern Techniques of Plant Analysis, Chapman and Hall Ltd., London, 116.
AOAC (1990) Association of Official Analytical Chemists. Official Methods of Analysis. 15th Edition, Washington DC.
Dorée, C. (1999) Les méthodes de la chimie de la cellulose. DUNOT, Paris. 406 p.
Egan, H., Kirk, R.S. and Sawyer, R. (1981) Pearson’s Chemical Analysis of Foods. 8th Edition, Churchill Livingstone, London, New York.
Pauwels, J.M., Van Panst, E., Verloo, M. and Mvondo Zé, A. (1992) Manuel de Laboratoire de Pédologie -méthodes d’analyses de sols et de plantes; équipement et gestion des stocks de verrerie et de produits chimiques. Publications Agricoles nr. 28, A.G.C.D., Bruxelles, Belgium, 180 p. <br/〉http://hdl.handle.net/1854/LU-223183
Chemical Composition
Health
General Well-Being
Human
Chapman, H.D. and Pratt, F.P. (1961) Calcium and Magnesium by Titration Methods. In: Chapman, H.D. and Pratt, F.P., Eds., Methods of Analysis for Soils, Plants and Water, University of California, Public Division of Agricultural Sciences, Berkeley, 20.
Chapman, H.D. and Pratt, F.P. (1968) Ammonium Molybdate-Ammonium Vandate Method for Determination of Phosphorus. In: Chapman, H.D. and Pratt, F.P., Eds., Methods of Analysis for Soils, Plants and Water, University of California, Public Division of Agricultural Sciences, Berkeley, 169-170.
Friedewald, W.T., Levy, R.I. and Frederickson, D.S. (1972) Estimation of Concentration of the Low Density Lipoprotein Cholesterol in Plasma, without Use of Preparative Ultracentrifuge. Clinical Chemistry, 18, 499-502.
Morton, J.F. (1987) Roselle. In: Dowling Jr., C.F., Ed., Fruits of Warm Climates, Media Inc., Greensboro, 281-286.
Adanlawo, I.G. and Ajibade, V.A. (2006) Nutritive Value of Two Varieties of Roselle (Hibiscus sabdariffa) Calyces Soaked with Wood Ash. Pakistan Journal of Nutrition, 5, 555-557. <br/〉http://dx.doi.org/10.3923/pjn.2006.555.557
Amed, A.O., Isa, M.T., Ahmed, A.S. and Adamu, S.B. (2009) Studies on the Use of Trona in Improving the Taste of the Extract from Hibiscus sabdariffa Calyx. Nigerian Journal of Pharmaceutical Sciences, 8, 7-12.
Bahaeldeen, B.M., Abdelatif, A.S. and Abdelhafiz, A.D. (2012) Roselle (Hibiscus sabdariffa L.) in Sudan, Cultivation and Their Uses. Bulletin of Environment, Pharmacology and Life Sciences, 1, 48-54.
Modi, A.T. (2007) Growth Temperature and Plant Age Influence on Nutritional Quality of Amaranthus Leaves and Seed Germination Capacity. Crop Science, School of Agricultural Sciences and Agribusiness, University of KwaZulu-Natal, Scottsville, South Africa.
Taiz, L. and Zeiger, E. (2002) Plant Physiology. 3rd Edition, Sinauer Associates Inc., Sunderland, 555-557.
Mady, C., Manuel, D., Mama, S., Augustin, N., Max, R. and Oumar, S. (2009) Le Bissap (Hibiscus sabdariffa L.): Composition et Principales Utilisations. Fruits, 64, 179-193. <br/〉http://dx.doi.org/10.1051/fruits/2009013
Asante, W.J., Nasare, I.L., Tom-Dery, D., Ochire-Boadu, K. and Kentil, K.B. (2014) Nutrient Composition of Moringa oleifera Leaves from Two Agro Ecological Zones in Ghana. African Journal of Plant Science, 8, 65-71. <br/〉http://dx.doi.org/10.5897/AJPS2012.0727
Mary, A.L. (2004) Anthocyanins and Human Health: An in Vitro Investigative Approach. Journal of Biomedicine and Biotechnology, 5, 306-313.
Asquith, T.N. and Butler, L.G. (1986) Interactions of Condensed Tannins with Selected Proteins. Photochemistry, 25, 1591-1593. <br/〉http://dx.doi.org/10.1016/S0031-9422(00)81214-5
Osuagwu, G.G.E., Okwulehie, I.C. and Emenike, J.O. (2007) Photochemical and Mineral Content of the Leaves of Four Nigerian Pterocarpus species. International Journal of Molecular Medicine and Advance Sciences, 3, 6-11.
Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E., Hilpert, K.F., Griel, A.E. and Etherton, T.D. (2002) Bioactive Compounds in Foods: Their Role in the Prevention of Cardiovascular Disease and Cancer. American Journal of Medicine, 113, 71S-88S. <br/〉http://dx.doi.org/10.1016/S0002-9343(01)00995-0
Alaa, G.A. (2012) Antioxidant and Antibacterial Activities of Hibiscus sabdariffa L. Extracts. African Journal of Food Science, 6, 506-511.
Kim, S.Y., Kim, J.H., Kim, S.K., Ohandy, M.J. and Jung, M.Y. (1994) Antioxidant Activities of Selected Oriental Herb Extracts. Journal of the American Oil Chemists’ Society, 71, 633-640. <br/〉http://dx.doi.org/10.1007/BF02540592
Mozaffari-Khosravi, H., Jalali-Khanabadi, B.A., Afkhami-Ardekani, M., Fatehi, F. and Noori-Shadkam, M. (2009) The Effects of Sour Tea (Hibiscus sabdariffa) on Hypertension in Patients with Type II Diabetes. Journal of Human Hypertension, 23, 48-54. <br/〉http://dx.doi.org/10.1038/jhh.2008.100
Haji Faraji, M. and Haji Tarkhani, A.H. (1999) The Effect of Sour Tea (Hibiscus sabdariffa) on Essential Hypertension. Journal of Ethnopharmacology, 65, 231-236. <br/〉http://dx.doi.org/10.1016/S0378-8741(98)00157-3
Ohlsson, A. and Aher, S.M. (2009) Early Erythropoietin for Preventing Red Blood Cells Transfusion in Preterm and/or Low Birth Weight Infants. Journal of Dietary Supplements, 6, 227-251.
Heda, G.L. and Bhatia, A.L. (1986) Hemocytometrical Changes in Swiss Albino Mice after Intrauterine Low Level HTO Exposure. Proc. Asian Reg. Conf. Med. Phys, 390.
Kaur, C. and Kapoor, H.C. (2005) Antioxidant Activity of Some Fruits in Indian Diet. Acta Horticulturae, 696, 563-565. VII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics—Part Two. <br/〉http://www.actahort.org/books/696/696_99.htm <br/〉http://dx.doi.org/10.17660/actahortic.2005.696.99
Ejere, V.C., Nnamonu, E.I., Chukwuka, C.O., Ugwu, G.C., Ejim, A.O. and Asogwa, C.N. (2013) Effects of Aqueous Extract of Hibiscus sabdariffa Calyces on Hematological Characteristics of Rattus Novergicus. Animal Research International, 10, 1809-1816.
Ots, I., Murumagi, A. and Horak, P. (1998) Hematological Health State Indices of Reproducing Great Tits: Methodology and Sources of Natural Variation. Functional Ecology, 12, 700-707. <br/〉http://dx.doi.org/10.1046/j.1365-2435.1998.00219.x
Adebayo, J.O., Adesokan, A.A., Olatunji, L.A., Burro, D.O. and Soladoye, A.O. (2005) Effect of Ethanolic Extract of Bougainvillea spectabilis Leaves on Hematological and Serum Lipid Variables in Rats. Biochemistry, 17, 45-50.
Ahamefule, F.O., Obua, B.E., Ukweni, I.A., Oguike, M.A. and Amaka, R.A. (2008) Hematological and Biochemical Profile of Weaner Rabbits Fed Raw or Processed Pigeon Pea Seed Meal Based Diets. African Journal of Agricultural Research, 3, 315-319.
Barger, A.M. (2003) The Complete Blood Cell Count: A Powerful Diagnostic Tool. Veterinary Clinics of North America: Small Animal Practice, 33, 1207-1222. <br/〉http://dx.doi.org/10.1016/S0195-5616(03)00100-1
Dhuley, J., Naik, S.R., Rele, S. and Banerji, A. (1999) Hypo Lipidemic and Antioxidant Activity of Di Allyl Disulphide in Rats. Pharmacy and Pharmacology Communications, 5, 689.
Lin, T.-L., Lin, H.-H., Chen, C.-C., Lin, M.-C., Chou, M.-C. and Wang, C.-J. (2007) Hibiscus sabdariffa Extract Reduces Serum Cholesterol in Men and Women. Nutrition Research, 27, 140-145. <br/〉http://dx.doi.org/10.1016/j.nutres.2007.01.007
Pooja, C.O. and Priscilla, D.M. (2009) Antioxidant and Anti Hyperlipidemic Activity of Hibiscus sabdariffa Linn. Leaves and Calyces Extracts in Rats. Indian Journal of Experimental Biology, 47, 276-282.
Adrian, P.H., Ross, G.C., Moustafa, A.S. and Usama, A. (2009) The Efficacity of Karkadey Tea in Controlling Post-Prandial Blood Glucose Levels. American Journal of Pharmacology and Toxicology, 4, 151-157. <br/〉http://dx.doi.org/10.3844/ajptsp.2009.151.157
Hansawasdi, C., Kawabata, J. and Kasai, T. (2000) Alpha-Amylase Inhibitors from Roselle (Hibiscus sabdariffa Linn) Tea. Bioscience, Biotechnology, and Biochemistry, 64, 1041-1043. <br/〉http://dx.doi.org/10.1271/bbb.64.1041
Ologundudu, A., Ologundudu, A.O., Ololade, I.A. and Obi, F.O. (2009) Effect of Hibiscus sabdariffa Anthocyanins on 2,4-Dinitrophenylhydrazine-Induced Hepatotoxicity in Rabbits. International Journal of Physical Sciences, 4, 233-237.
Onyenekwe, P.C., Ajani, E.O., Ameh, D.A. and Gamaniel, K.S. (1999) Antihypertensive Effect of Roselle (Hibiscus sabdariffa) Calyx Infusion in Spontaneously Hypertensive Rats and a Comparison of Its Toxicity with That in Wistar Rats. Cell Biochemistry and Function, 17, 199-206. 3.0.CO;2-2
Qiang, L., Xiaoli, L., Shuhua, L., Changshun, X., Yali, L. and Chunming, M. (2012) The Relationship between AST/ALT Ratio and Metabolic Syndrome in Han Young Adults-AST/ALT Ratio and Metabolic Syndrome. Recent Advances in Cardiovascular Risk Factors, 12, 248-254.