<i>In Vitro </i>Effects of Oil’s Fatty Acids on T-Cell Function of Obese Men — Oak Academic Publishing
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<i>In Vitro </i>Effects of Oil’s Fatty Acids on T-Cell Function of Obese Men
Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
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Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
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Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
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Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
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Department of Technical Sciences, Faculty of Engineering, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
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Laboratory of Biotoxicology, Djilali Liabes University, Sidi Bel Abbès, Algeria
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école de Nutrition, Université de Moncton, New Brunswick, Canada
1 Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
2 Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
3 Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
4 Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
5 Department of Technical Sciences, Faculty of Engineering, University of Abou-Bekr Belkaïd, Tlemcen, Algeria
Background: Dietary fatty acids have important homeostatic functions in regulating the immune response and may exert beneficial effects on immune alterations during obesity. Objective: To assess the in vitro effects of oil fatty acids, different oils (olive, linseed, Nigel, sunflower) were tested on T-lymphocyte proliferation, Th1- and Th2-type cytokine production, and intracellular oxidant/antioxidant status in obese patients. Methods: Peripheral blood lymphocytes were isolated using Histopaque and were in vitro cultured and stimulated by Con A in the presence or absence of the oils. Cell proliferation, interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions and intracellular oxidative status (glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronuclei frequency) were investigated. Results: Abnormalities in lymphocyte function and intracellular oxidative stress were observed in obesity. Linseed oil induced a reduction in T-lymphocyte proliferation and IL-2 production while Nigel oil increased them in both obese and control groups. In addition, Nigel oil enhanced IFNγ and IL-4 secretion. Olive and sunflower oils had no effect on lymphocyte proliferation and cytokine secretion in both groups. Linseed and Nigel oils induced an increase in T cell GSH concentrations and catalase activity with a concomitant decrease in MDA, carbonyl protein contents and micronuclei frequency especially in obese patients. Conclusion: Linseed and Nigel oils had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, while olive and sunflower oils had no effects on immune cell function in obesity.
KeywordsObesityLymphocytesOlive OilLinseed OilNigel OilSunflower OilCytokinesOxidant/Antioxidant Status
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