The effects of nutrients and physical conditions on phytase production were investigated with a recently isolated strain of Aspergillus tubingensis SKA under solid state fermentation on wheat bran. The nutrient factors investigated included carbon source, nitrogen source, phosphate source and concentration, metal ions (salts) and the physical parameters investigated included inoculum size, pH, temperature and fermentation duration. Our investigations revealed that optimal productivity of phytase was achieved using wheat bran supplemented with: 1.5% glucose. 0.5% (NH 4 ) 2 SO 4 , 0.1% sodium phytate. Additionally, optimal physical conditions were 1 × 10 5 spore/g substrate, initial pH of 5.0, temperature of fermentation 30°C and fermentation duration of 96 h. Overall, a 34% improvement in phytase activity was achieved by using the optimal conditions.
KeywordsPhytase<i>Aspergillus tubingensis</i>SKASolid State FermentationOptimization
Vats, P. and Banerjee, U.C. (2004) Production Studies and Catalytic Properties of Phytases (myo-Inositol Hexakisphosphate Phosphohydrolases): An Overview. Enzyme and Microbial Technology, 35, 3-14. https://doi.org/10.1016/j.enzmictec.2004.03.010
Santos, M.M., de Rosa, A.S., Dal’boit, S., Mitchell, D.A. and Kriger, N. (2004) Thermal Denaturation: Is Solid-State Fermentation Really a Good Technology for the Production of Enzymes. Bioresource Technology, 99, 261-268. https://doi.org/10.1016/j.biortech.2003.11.007
Rostami, H. and Giri, A. (2013) An Overview on Microbial Phytases and Its Biotechnological Application. International Journal of Advanced Biotechnology and Research, 4, 62-71.
Soni, S.K., Magdum, A. and Khire, J.M. (2010) Purification and Characterization of Two Distinct Acidic Phytases with Broad pH Stability from Aspergillus niger NCIM 563. World Journal of Microbiology and Biotechnology, 26, 2009-2010. https://doi.org/10.1007/s11274-010-0385-8
Liu, N., Ru, Y., Wang, J. and Xu, T. (2010) Effect of Dietary Sodium Phytate and Microbial Phytase on the Lipase Activity and Lipid Metabolism of Broiler Chickens. British Journal of Nutrition, 103, 862-868. https://doi.org/10.1017/S0007114509992558
Subramaniyam, R. and Vimala, R. (2012) Solid State and Submerged Fermentation for the Production of Bioactive Substances: A Comparative Study. International Journal of Science and Nature, 3, 480-486.
Muniswaran, P.K.A., Selvakumar, P. and Charyulu, N.C.L.N. (1994) Production of Cellulases from Coconut Coir Pith in Solid-State Fermentation. A Comparative Study. Journal of Chemical Technology and Biotechnology, 60, 147-151. https://doi.org/10.1002/jctb.280600206
Zhu, Y., Smit, J.P., Knol, W. and Bol, J. (1994) A Novel Solid-State Fermentation System Using Polyurethane Foam as Inter Carrier. Biotechnology Letters, 16, 643-648. https://doi.org/10.1007/BF00128615
Becerra, M. and Gonzalez Siso, M.I. (1996) Yeast β-Galactosidase in Solid-State Fermentation. Enzyme and Microbial Technology, 19, 39-44. https://doi.org/10.1016/0141-0229(95)00180-8
El-Batal, A.I. and Abdel Karem, H. (2001) Phytase Production and Phytic Acid Reduction in Rapeseed Meal by Aspergillus niger during Solid-State Fermentation. Food Research International, 34, 715-720. https://doi.org/10.1016/S0963-9969(01)00093-X
Bhavsar, K. Shah, P., Soni, S.K. and Khire, J.M. (2008) Influence of Pretreatment of Agriculture Residues on Phytases Production by Aspergillus niger NCIM 563 under Submerged Fermentation Conditions. African Journal of Biotechnology, 7, 1101-1106.
Lata, S., Rostogi, S., Kapoor, A. and Imran, M. (2013) Optimization of Culture Conditions for the Production of Phytase from Aspergillus heteromorphous MTCC 10685. International Journal of Advanced Biotechnology and Research, 4, 224-235.
Awad, G.E.A., Helal, M.M.I., Danial, E.N. and Esawy, M.A. (2014) Optimization of Phytase Production by Penicillium purpurogenum GE1 under Solid-State Fermentation by Using Box-Behnken Design. Saudi Journal of Biological Sciences, 21, 81-88.
Das, S. and Ghosh, U. (2014) Effect of Nutritional Supplementation of Solid-State Fermentation Medium on Biosynthesis of Phytase from Aspergillus niger NCIM 612. Journal of Scientific and Industrial Research, 73, 593-597.
Gaind, S. and Singh, S. (2015) Production, Purification and Characterization of Neutral Phytase from Thermotolerant Aspergillus flavus ITCC 6720. International Bioremediation and Biodegradation, 99, 15-22.
Fiske, C.H. and Subbarow, Y. (1925) The Colorimetric Determination of Phosphorus. The Journal of Biological Chemistry, 2, 375-400.
Qasim, S.S., Shakir, K.A. and Al-Shaibani, A.B. (2016) Isolation, Screening and Production of Phytate Degrading Enzyme (Phytase) from Local Fungal Isolate. The Iraqi Journal of Agricultural Sciences, 47, 121-128.
El-Gindy, A.A., Ibrahim, Z.M., Ali, U.F. and El-Mahdy, O.M. (2009) Extracellular Phytase Production by Solid-State Cultures of Malbranchea sulfurea and Aspergillus niveus on Cost-Effective Medium. Research Journal of Agriculture and Biological Sciences, 5, 42-62.
Awad, G.E.A., Elnashar, M.M.M. and Danial, E.N. (2011) Optimization of Phytase Production by Penicillium funiculosum NRC467 under Solid-State Fermentation by Using Full Factorial Design. World Applied Sciences Journal, 15, 1635-1644.
Jafari-Tapeh, H., Hamidi-Esfahani, Z. and Azizi, M.H. (2012) Culture Condition Improvement for Phytase Production in Solid-State Fermentation by Aspergillus ficuum Using Statistical Method. ISRN Chemical Engineering, 2012, Article ID: 479167. https://doi.org/10.5402/2012/479167
Roopesh, K., Ramachandran, S., Nampoothiri, K.M., Szakacs, G. and Pandey, A. (2006) Comparison of Phytases Production on Wheat Bran and Oilcakes in Solid-State Fermentation by Mucor racemosus. Bioresource Technology, 97, 506-511.
Kumari, M.P., Kumar, M.S. and Rao, G.N. (2011) Isolation of Phytase Producing Fungi and Optimization of Production Parameters. Journal of Global Trends in Pharmaceutical Sciences, 2, 161-176.
Bala, A., Sapna, Jain, J., Kumari, A. and Singh, B. (2014) Production of an Extracellular Phytase from a Thermophilic Mould Humicola nigrescens in Solid-State Fermentation and Its Application in Dephytinization. Biocatalysis and Agricultural Biotechnology, 3, 259-264.
Kim, D., Godber, J.S. and Kim, H. (1999) Culture Conditions for New Phytases-Producing Fungus. Biotechnology Letters, 21, 1077-1081. https://doi.org/10.1023/A:1005696829168
Selvamohan, T., Ramadas, V. and Rejibeula, M. (2012) Optimization of Phytase Production by Pseudomonas sp. Isolated from Poultry Faces. International Journal of Modern Engineering Research, 2, 1326-1330.
Tungala, A., Narayanan, K.A. and Muthraman, M.S. (2013) Isolation of Phytases Producing Bacteria from Poultry Feaces and Optimization of Culture Conditions for Enhanced Phytase Production. International Journal of Pharmacy and Pharmaceutical Sciences, 5, 264-269.
Nakamura, Y., Fukuhara, H. and Sano, K. (2000) Secreted Phytase Activities of Yeast. Bioscience Biotechnology and Biochemistry, 64, 841-844. https://doi.org/10.1271/bbb.64.841
Soni, S.K. and Khire, J.M. (2007) Production and Partial Purification of Two Types of Phytase from Aspergillus niger NCIM 563 under Submerged Fermentation Conditions. World Journal of Microbiology and Biotechnology, 23, 1585-1593. https://doi.org/10.1007/s11274-007-9404-9
Mittal, A., Singh, G., Goyal, V., Yadava, A. and Aggarwal, N.K. (2012) Production of Phytase by Acido-Thermophilic Strain of Klebsiella sp. DB-3FJ711774.1 Using Orange Peel Flour under Submerged Fermentation. Innovative Romanian Food Biotechnology, 10, 18-27.
Sreedevi, S. and Reddy, B.N. (2012) Isolation, Screening and Optimization of Phytases Production from Newly Isolated Bacillus sp.C43. International Journal of Pharmacy and Biological Sciences, 2, 218-231.
Vohra, A. and Satyanarayana, T. (2003) Phytases: Microbial Sources, Production, Purification and Potential Biotechnological Applications. Critical Reviews in Biotechnology, 23, 29-60. https://doi.org/10.1080/713609297
Singh, B. and Satyanaryana, T. (2012) Production of Phytate-Hydrolyzing Enzymes by Theromphiloic Moulds. African Journal of Biotechnology, 11, 12314-12324.
Ramachandran, S., Roopesh, K., Namoopthiri, K.M., Sazackas, G. and Pandey, A. (2005) Mixed Substrate Fermentation for the Production of Phytases by Rhizopus spp. Using Oilcake as Substrate. Process Biochemistry, 40, 1749-1754.
Sabu, A., Sarita, S., Pandey, A., Bogar, B., Szakasc, G. and Soccol, C.R. (2002) Solid-State Fermentation for Production of Phytases by Rhizopus oligosporus. Applied Biochemistry and Biotechnology, 102, 251-260. https://doi.org/10.1385/ABAB:102-103:1-6:251
Lee, D.-H., Choi, S.-U. and Hwang, Y.-I. (2005) Culture Conditions and Characterizations of a New Phytase-Producing Fungal Isolate, Aspergillus sp. L117. Mycobiology, 33, 223-229. https://doi.org/10.4489/MYCO.2005.33.4.223
Singh, S.B. (2014) Phytase Production by Aspergillus oryzae in Solid-State Fermentation and Its Application in Dephytinization of Wheat Bran. Applied Biochemistry and Biotechnology, 173, 1293-1303. https://doi.org/10.1007/s12010-014-1021-0
Papagianni, M., Nokes, S.E. and Filer, K. (2001) Submerged and Solid-State Phytase Fermentation by Aspergillus niger: Effects of Agitation Medium Viscosity on Phytase Production, Fungal Morphology and Inoculum Performance. Food Technology and Biotechnology, 39, 319-326.
Casey, A. and Walsh, G. (2003) Purification and Characterization of Extracellular Phytases from Aspergillus niger ATCC 9142. Bioresource Technology, 86, 183-188.
Hassouni, H. Ismaili-Alaoui, M. Gaime-Perraud, I. Augur, C. and Roussos, S. (2006) Effect of Culture Media and Fermentation Parameters on Phytase Production the Thermophilic Fungus Myceliophthora thermophile in Solid-State Fermentation. Micologia Aplicada International, 18, 29-36.
Badamchi, M., Hamidi-Esfahani, Z. and Abbasi, S. (2013) Comparison of Phytase Production by Aspergillus ficuum under Submerged and Soil-State Fermentation Conditions. Focusing on Modern Food Industry, 2, 129-137.
Moreira, K.A., Herculano, P.N., Maciel, M.H.C., Porto, T.S., Spier, M.R., Souza-Motta, C.M., Porto, L.F. and Soccoi, C.R. (2014) Optimization of Phytase Production by Aspergillus japonicas Saito URM 5633 Using Cassava Bast as Substrate in Solid-State Fermentation. African Journal of Microbiology Research, 8, 929-938. https://doi.org/10.5897/AJMR2013.6565
Shivanna, G.B. and Venkateswaran, G. (2014) Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-Stat Fermentation. The Scientific World Journal, 2014, Article ID: 392615. https://doi.org/10.1155/2014/392615
Rani, R., Arora, S., Kumar, S. and Ghosh, S. (2013) Optimization of Medium Components for the Production of Phytase by R. oryzae Using Stastical Approaches. Journal of Bioremediation and Biodegradation, S18, 003. https://doi.org/10.4172/2155-6199.S18-003
Thyagarajan, R., Namasivayam, S.K.R. and Kumar, G.N. (2014) Optimization of Medium Component for Phytase Production by Hypocrea lixii SURT01 Using Response Surface Methodology. Journal of Pure and Applied Microbiology, 8, 2485-2490.