Plant material screening was performed to study anti-Helicobacter pylori activity in vitro using an agar diffusion method on Columbia blood agar. 33 substances, juices and plant extracts and 35 of their combinations were tested. Quince (Cydonia oblonga) juice demonstrated the strongest anti-H. pylori activity followed by cranberry juice. Con-centrated apple juice, plum, red currant, black chokeberry, raspberry and bilberry juice also showed significant activity. Green tea and apple pomace extract as well as sweet flag rhizome, ginger and wild bergamot extract, cherry syrup, red beet juice and whey did not exhibit anti-Helicobacter activity. Quince juice in combination with bilberry, black chokeberry, red currant juice, green tea, sweet flag rhizome or apple pomace extract as well as cranberry juice in combination with sweet flag rhizome extract demonstrated a synergistic effect on inhibition of H. pylori. The obtained results offer new perspectives for development of functional anti-Helicobacter food product(s) for dietary management of H. pylori infection. The essential components of these products could be the most active juices and extracts like quince and cranberry juice supplemented with a corresponding synergist. Further studies are required to investigate the mechanism of antibacterial action of plant products and their efficacy in vivo.
C. C. McGowan, T. L. Cover and M. J. Blaser, “Helicobacter pylori and Gastric Acid: Biological and Therapeutic Implications,” Gastroenterology, Vol. 110, No. 3, 1996, pp. 926-938. doi:10.1053/gast.1996.v110.pm8608904
D. S. Merrell, M. L. Goodrich, G. Otto, L. S. Tompkins and S. Falkow, “pH-Regulated Gene Expression of the Gastric Pathogen Helicobacter pylori,” Infection and Immunity, Vol. 71, No. 6, 2003, pp. 3529-3539. doi:10.1128/IAI.71.6.3529-3539.2003
S. Perry, M. L. Sanchez, S. Yang, T. D. Haggerty, P. Hurst, G. Perez-Perez and J. Parsonnet, “Gastroenteritis and Transmission of Helicobacter pylori Infection in Households,” Emerging Infectious Diseases, Vol. 12, No. 11, 2006, pp. 1701-1708.
H. M. Malaty, A. El-Kasabany, D. Y. Graham, C. C. Miller, S. G. Reddy, S. R. Srinivasan, Y. Yamaoka and G. S. Berenson, “Age at Acquisition of Helicobacter pylori Infection: A Follow-Up Study from Infancy to Adulthood,” The Lancet, Vol. 359, No. 9310, 2002, pp. 931-535. doi:10.1016/S0140-6736(02)08025-X
T. Sabbi, P. de Angelis and L. Dall’Oglio, “Helicobacter pylori Infection in Children: Management and Pharmacotherapy,” Expert Opinion on Pharmacotherapy, Vol. 9, No. 4, 2008, pp. 577-585. doi:10.1517/14656566.9.4.577
L. M. Brown, “Helicobacter pylori: Epidemiology and Routes of Transmission,” Epidemiologic Reviews, Vol. 22, No. 2, 2000, pp. 283-297.
F. F. Vale and J. M. Vitor, “Transmission Pathway of Helicobacter pylori: Does Food Play a Role in Rural and Urban Areas?” International Journal of Food Microbiology, Vol. 138, No. 1-2, 2010, pp. 1-12. doi:10.1016/j.ijfoodmicro.2010.01.016
L. Fischbach and E. L. Evans, “Meta-Analysis: The Effect of Antibiotic Resistance Status on the Efficacy of Triple and Quadruple First-Line Therapies for Helicbacter pylori,” Alimentary Pharmacology and Therapeutics, Vol. 26, No. 3, 2007, pp. 343-357. doi:10.1111/j.1365-2036.2007.03386.x
D. Y. Graham and A. Shiotani, “New Concepts of Resistance in the Treatment of Helicobacter pylori Infections,” Nature Clinical Practice Gastroenterology and Hepatology, Vol. 5, No. 6, 2008, pp. 321-331. doi:10.1038/ncpgasthep1138
B. Stenstrom, A. Mendis and B. Marshall, “Helicobacter pylori—the Latest in Diagnosis and Treatment,” Australian Family Physician, Vol. 37, No. 8, 2008, pp. 608-612.
M. P. Della, A. Lavagna, G. Masoero, L. Lombardo, L. Crocella and A. Pera, “Effectiveness of Helicobacter pylori Eradication Treatments in a Primary Care Setting in Italy,” Alimentary Pharmacology and Therapeutics, Vol. 16, No. 7, 2002, pp. 1269-1275. doi:10.1046/j.1365-2036.2002.01244.x
M. Heep, M. Kist, S. Strobel, D. Beck and N. Lehn, “Secondary Resistance among 554 Isolates of Helicobacter pylori after Failure of Therapy,” European Journal of Clinical Microbiology and Infectious Diseases, Vol. 19, No. 7, 2000, pp. 538-541. doi:10.1007/s100960000288
F. Bazzoli, P. Pozzato and T. Rokkas, “Helicobacter pylori: The Challenge in Therapy,” Helicobacter, Vol. 7, Suppl. 1, 2002, pp. 43-49. doi:10.1046/j.1523-5378.7.s1.7.x
A. Qasim and C. A. O’Morain, “Review Article: Treatment of Helicobacter pylori Infection and Factors Influencing Eradication,” Alimentary Pharmacology and Therapeutics, Vol. 16, 2002, pp. 24-30. doi:10.1046/j.1365-2036.2002.0160s1024.x
R. N. Ndip, A. E. M. Tarkang, C. M. Echakachi, A. Malongue, J.-F. T. K. Akoachere, L. M. Ndip and H. N. Luma, “In vitro Antimicrobial Activity of Selected Honeys on Clinical Isolates of Helicobacter pylori,” African Health Sciences, Vol. 7, No. 4, 2007, pp. 228-231.
R. N. Ndip, A. E. M. Tarkang, S. M. Mbullah, H. N. Luma, A. Malongue, L. M. Ndip, K. Nyongbela, C. Wirmum and S. M. N. Efange, “In Vitro Anti-Helicobacter pylori Activity of Extracts of Selected Medicinal Plants from North West Cameroon,” Journal of Ethnopharmacology, Vol. 114, No. 3, 2007, pp. 452-457. doi:10.1016/j.jep.2007.08.037
C. F. Carson and T. V. Riley, “Non-Antibiotic Therapies for Infectious Diseases,” Communicable Diseases Intelligence, Vol. 27, 2003, pp. S143-146.
M. M. Kamiji and R. B. Oliveira, “Non-Antibiotic Therapies for Helicobacter pylori Infection,” European Journal of Gastroenterology and Hepatology, Vol. 17, No. 9, 2005, pp. 973-981. doi:10.1097/00042737-200509000-00014
J. I. Keenan, N. Salm, M. B. Hampton and A. J. Wallace, “Individual and Combined Effects of Foods on Helicobacter pylori Growth,” Phytotherapy Research, Vol. 24, No. 8, 2010, pp. 1229-1233.
S. Sadeghian, T. R. Neyestani, M. H. Shirazi and P. Ranjbarian, “Bacteriostatic Effect of Dill, Fennel, Caraway and Cinnamon Extracts against Helicobacter pylori,” Journal of Nutritional and Environmental Medicine, Vol. 15, No. 2-3, 2005, pp. 47-55. doi:10.1080/13590840500535313
S. Y. Lee, Y. W. Shin and K. B. Hahm, “Phytoceuticals: Mighty but Ignored Weapons against Helicobacter pylori Infection,” Journal of Digestive Diseases, Vol. 9, No. 3, 2008, pp. 129-139. doi:10.1111/j.1751-2980.2008.00334.x
H. S. Falcao, I. R. Mariath, M. F. Diniz, L. M. Batista and J. M. Barbosa-Filho, “Plants of the American Continent with Antiulcer Activity,” Phytomedicine, Vol. 15, No. 1-2, 2008, pp. 132-146. doi:10.1016/j.phymed.2007.07.057
A. Yanaka, J. W. Fahey, A. Fukumoto, M. Nakayama, S. Inoue, S. Zhang, M. Tauchi, H. Suzuki, I. Hyodo and M. Yamamoto, “Dietary Sulforaphane-Rich Broccoli Sprouts Reduce Colonization and Attenuate Gastritis in Helicobacter pylori—Infected Mice and Humans,” Cancer Prevention Research, Vol. 2, No. 4, 2009, pp. 353-360.
A. Chatterjee, T. Yasmin, D. Bagchi and S. J. Stohs, “Inhibition of Helicobacter pylori in vitro by Various Berry Extracts, with Enhanced Susceptibility to Clarithromycin,” Molecular and Cellular Biochemistry, Vol. 265, No. 1-2, 2004, pp. 19-26. doi:10.1023/B:MCBI.0000044310.92444.ec
H. Shmuely, J. Yahav, Z. Samra, G. Chodick, R. Koren, Y. Niv and I. Ofek, “Effect of Cranberry Juice on Eradication of Helicobacter pylori in Patients Treated with Antibiotics and a Proton Pump Inhibitor,” Molecular Nutrition and Food Research, Vol. 51, No. 6, 2007, pp. 746-751. doi:10.1002/mnfr.200600281
S. Enomoto, K. Yanaoka, H. Utsunomiya, T. Niwa, K. Inada, H. Deguchi, K. Ueda, C. Mukoubayashi, I. Inoue, T. Maekita, K. Nakazawa, M. Iguchi, K. Arii, H. Tamai, N. Yoshimura, M. Fujishiro, M. Oka and M. Ichinose, “Inhibitory Effects of Japanese Apricot (Prunus mume Siebold et Zucc.; Ume) on Helicobacter pylori-Related Chronic Gastritis,” European Journal of Clinical Nutrition, Vol. 64, No. 7, 2010, pp. 714-719. doi:10.1038/ejcn.2010.70
G. B. Mahady, S. L. Pendland, A. Stoia, F. A. Hamill, D. Fabricant, B. M. Dietz and L. R. Chadwick, “In Vitro Susceptibility of Helicobacter pylori to Botanical Extracts Used Traditionally for the Treatment of Gastrointestinal Disorders,” Phytotherapy Research, Vol. 19, No. 11, 2005, pp. 988-991. doi:10.1002/ptr.1776
Otsuka Pharmaceutical Co., Ltd (JP), Inventors: M. Kikuchi, H. Takiza, H. Wakebe, F. Mukai, S. Shimizu, H. Okamatsu, T. Saito, S. Yamadaira, H. Kimura and K. Sasaki, “Composition, Antimicrobial Agent, Infection Preventive, and Food against Helicobacter pylori,” WIPO Patent Application WO/1998/009652, Application Number: PCT/JP/1997/ 003100. 1997.09.04.
M. Tabak, R. Armon, G. Rosenblat, E. Stermer and I. Neeman, “Diverse Effects of Ascorbic Acid and Palmitoyl Ascorbate on Helicobacter Pylori Survival and Growth,” FEMS Microbiology Letters, Vol. 224, No. 2, pp. 247-253. doi:10.1016/S0378-1097(03)00439-7
C. H. Chuang, B. S. Sheu, A. W. Kao, H. C. Cheng, A. H. Huang, H. B. Yang and J. J. Wu, “Adjuvant Effect of Vitamin C on Omeprazole-Amoxicillin-Clarithromycin Triple Therapy for Helicobacter pylori Eradication,” Hepato-Gastroenterology, Vol. 54, No. 73, 2007, pp. 320-324.
L. Zhang, J. Ma, K. Pan, V. L. Go, J. Chen and W. C. You, “Efficacy of Cranberry Juice on Helicobacter pylori Infection: Randomised Placebo-Controlled Trial,” Helicobacter, Vol. 10, No. 2, 2005, pp. 139-145. doi:10.1111/j.1523-5378.2005.00301.x
O. Burger, I. Ofek, M. Tabak, E. Weiss, N. Sharon and I. Neeman, “A High Molecular Mass Constituent of Cranberry Juice Inhibits Helicobacter pylori Adhesion to Human Gastric Mucus,” FEMS Immunology and Medical Microbiology, Vol. 29, No. 4, 2000, pp. 295-301. doi:10.1111/j.1574-695X.2000.tb01537.x
S. Fattouch, P. Caboni, V. Coroneo, C. I. G. Tuberoso, A. Angioni, S. Dessi, N. Marzouki and P. Cabras, “Antimicrobial Activity of Tunisian Quince (Cydonia oblonga Miller) Pulp and Peel Polyphenolic Extracts,” Journal of Agricultural and Food Chemistry, Vol. 55, No. 3, 2007, pp. 963-969. doi:10.1021/jf062614e
F. Aquil and I. Ahmad, “Antibacterial Properties of Traditionally Used Indian Medicinal Plants,” Methods and Findings in Experimental and Clinical Pharmacology, Vol. 29, No. 2, 2007, pp. 79-92. doi:10.1358/mf.2007.29.2.1075347
H. C. Lee, A. M. Jenner, C. S. Low and Y. K. Lee, “Effect of Tea Phenolics and their Aromatic Fecal Bacterial Metabolites on Intestinal Microbiota,” Research in Microbiology, Vol. 157, No. 9, 2006, pp. 876-884. doi:10.1016/j.resmic.2006.07.004
A. Rocco and G. Nardone, “Diet, H. pylori Infection and Gastric Cancer: Evidence and Controversies,” World Journal of Gastroenterology, Vol. 13, No. 21, 2007, pp. 2901-2912.
C. Stoicov, R. Saffari and J. Houghton, “Green Tea Inhibits Helicobacter Growth in Vivo and in Vitro,” International Journal of Antimicrobial Agents, Vol. 33, No. 5, 2009, pp. 473-478. doi:10.1016/j.ijantimicag.2008.10.032
E. Pastene, H. Speisky, M. Troncoso, J. Alarcon and G. Figueroa, “In vitro Inhibitory Effect of Apple Peel Extract on the Growth of Helicobacter pylori and Respiratory Burst Induced on Human Neutrophils,” Journal of Agricultural and Food Chemistry, Vol. 57, No. 17, 2009, pp. 7743-7749. doi:10.1021/jf9006592
D. A. Vattem, Y. T. Lin, R. Ghaedian and K. Shetty, “Cranberry Synergies for Dietary Management of Helicobacter pylori Infections,” Process Biochemistry, Vol. 40, No. 5, 2005, pp. 1583-1592. doi:10.1016/j.procbio.2004.06.024