Evaluation of Organic Acid-Based Sanitizers for Reduction of <i>Escherichia coli</i> O157:H7 during Flume-Washing of Organic Leafy Greens — Oak Academic Publishing
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Evaluation of Organic Acid-Based Sanitizers for Reduction of <i>Escherichia coli</i> O157:H7 during Flume-Washing of Organic Leafy Greens
Dept. of Animal Science and Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma, USA
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Dept. of Animal Science and Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma, USA
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Dept. of Animal Science and Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma, USA
1 Dept. of Animal Science and Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma, USA
2 Dept. of Animal Science and Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma, USA
3 Dept. of Animal Science and Robert M. Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma, USA
Antimicrobial efficacy of three novel organic sanitizers, CHICO Wash TM , C8C10 and CG100, was evaluated for the reduction of Escherichia coli O157:H7 during flume-washing of organic leafy greens. Organic formulations at various concentrations: CHICO (C 3 H 8 I 0 C 3 O 7 ) Wash TM (1:20 ratio) and C8C10 and CG100 (0.2 and 0.4%), along with the controls: hydrogen-peroxide and water, were used for washing organic baby and mature spinach and romaine and iceberg lettuce. Leafy greens were inoculated with a 2-strain cocktail of E. coli O157:H7 (6 logs CFU/mL) and washed in each treatment for 1 or 2 minutes. The treated leafy greens were stored at 4°C and surviving pathogen populations determined on days 0, 1, and 3 of storage. Organic sanitizers, for both treatment times, significantly ( P < 0.05) reduced E. coli O157:H7 on all the leafy greens during storage. Highest reduction (3.4 logs CFU/g) was observed after treatment with CG100 (0.4%) in romaine lettuce, while CHICO Wash TM showed greater than 2 logs CFU/g reduction on all the leafy greens, by day 3. This study demonstrates the potential application of organic sanitizers in flume-washing of organic leafy greens for the reduction of E. coli O157:H7.
Greene, C. (2013) Growth Patterns in the US Organic Industry. https://www.ers.usda.gov/amber-waves/2013/october/growth-patterns-in-the-us-organic-industry/
Organic Trade Association (OTA). (2015) Organic Industry Survey. https://www.ota.com/news/pressreleases/19031
United States Department of Agriculture—Economic Research Services (USDA-ERS). (2016) Organic Market Overview. https://www.ers.usda.gov/topics/natural-resources-environment/organic-agriculture/organic-market-overview/
Scallan, E., Hoekstra, R.M., Angulo, F.J., Tauxe, R.V., Widdowson, M.A., Roy, S.L., and Griffin, P.M. (2011) Foodborne Illness Acquired in the United States—Major Pathogens. Emerging Infectious Diseases, 17, 7-15. https://doi.org/10.3201/eid1701.P11101
Painter, J.A., Hoekstra, R.M., Ayers, T., Tauxe, R.V., Braden, C.R., Angulo, F.J., and Griffin, P.M. (2013) Attribution of Foodborne Illnesses, Hospitalizations, and Deaths to Food Commodities by Using Outbreak Data, United States, 1998-2008. Emerging Infectious Diseases, 19, 407-415. https://doi.org/10.3201/eid1903.111866
Centers for Disease Control and Prevention (CDC). (2012) Multi-State Outbreak of Shiga Toxin-Producing Escherichia Coli O157:H7 Infections Associated with Consumption of Organic Spinach and Spring Mix Blend. http://www.cdc.gov/ecoli/2012/O157H7-11-12/index.html
CDC. (2013) Multistate Outbreak of Hepatitis A Virus Infections Linked to Pomegranate Seeds from Townsend Farms Frozen Organic Antioxidant Blend. https://www.cdc.gov/hepatitis/outbreaks/2013/a1b-03-31/index.html
CDC. (2014) Multistate Outbreak of Salmonella Infections Linked to Organic Sprouted Chia Powder. http://www.cdc.gov/salmonella/newport-05-14/index.html
Slutsker, L., Altekruse, S.F. and Swerdlow, D.L. (1998) Foodborne Diseases: Emerging Pathogens and Trends. Infectious Disease Clinics in North America, 12, 199-216. https://doi.org/10.1016/S0891-5520(05)70418-9
Rangel, J.M., Sparling, P.H., Crowe, C., Griffin, P.M. and Swerdlow, D.L. (2005) Epidemiology of Escherichia coli O157:H7 Outbreaks, United States, 1982-2002. https://wwwnc.cdc.gov/eid/article/11/4/040739_article
Steele, M. and Odumeru, J. (2004) Irrigation Water as Source of Foodborne Pathogens on Fruit and Vegetables. Journal of Food Protection, 67, 2839-2849. https://doi.org/10.4315/0362-028X-67.12.2839
Farrar, J. and Guzewich, J. (2009) Identification of the Source of Contamination. In: Sapers, G.M., Solomon, E.B. and Matthews, K.R., Eds., The Produce Contamination Problem: Causes and Solutions, Academic Press, San Diego, 49-77.
Dimitri, C. and Greene, C. (2002) Recent Growth Patterns in the US Organic Foods Market. Agriculture Information Bulletin, 777, 1-39. https://www.ers.usda.gov/webdocs/publications/42455/13376_aib777b_1_.pdf?v=41055
United States Department of Agriculture National Organic Program (USDA-NOP). (2011) USDA Organic Regulations. Electronic Code of Federal Regulations, Title 7, Subtitle B, Chapter I, Subchapter M, Part 205. https://www.gpo.gov/fdsys/pkg/CFR-2011-title7-vol3/pdf/CFR-2011-title7-vol3-part205.pdf
Plotto, A. and Narciso, J.A. (2006) Guidelines and Acceptable Postharvest Practices for Organically Grown Produce. Hort Science, 41, 287-291.
Beuchat, L. (1998) Surface Decontamination of Fruits and Vegetables Eaten Raw: A Review. http://apps.who.int/iris/bitstream/10665/64435/1/WHO_FSF_FOS_98.2.pdf
Yu, K., Newman, M.C., Archbold, D.D. and Hamilton-Kemp, T.R. (2001) Survival of Escherichia coli O157:H7 on Strawberry Fruit and Reduction of the Pathogen Population by Chemical Agents. Journal of Food Protection, 64, 1334-1340. https://doi.org/10.4315/0362-028X-64.9.1334
Van Haute, S., Tryland, I., Veys, A. and Sampers, I. (2015) Wash Water Disinfection of a Full-Scale Leafy Vegetables Washing Process with Hydrogen-Peroxide and the Use of a Commercial Metal Ion Mixture to Improve Disinfection Efficiency. Food Control, 50, 173-183.
Sapers, G. and Sites, J. (2003) Efficacy of 1% Hydrogen-Peroxide Wash in Decontaminating Apples and Cantaloupe Melons. Journal of Food Science, 68, 1793-1797. https://doi.org/10.1111/j.1365-2621.2003.tb12331.x
Park, C. and Beuchat, L. (1999) Evaluation of Sanitizers for Killing Escherichia coli O157:H7, Salmonella, and Naturally Occurring Microorganisms on Cantaloupes, Honeydew Melons, and Asparagus. Dairy Environmental Sanitation, 19, 842-847.
Taormina, P.J. and Beuchat, L.R. (1999) Comparison of Chemical Treatments to Eliminate Escherichia coli O157:H7 on Alfalfa Seeds. Journal of Food Protection, 62, 318-324. https://doi.org/10.4315/0362-028X-62.4.318
Denton, J.J., Ravishankar, S., Friedman, M. and Jaroni, D. (2015) Efficacy of Plant-Derived Compounds against Escherichia coli O157:H7 during Flume-Washing and Storage of Organic Leafy Greens. Journal of Food Processing and Preservation, 39, 2728-2737. https://doi.org/10.1111/jfpp.12523
Lin, C.M., Moon, S.S., Doyle, M.P. and Mcwatters, K.H. (2002) Inactivation of Escherichia coli O157:H7, Salmonella enterica Serotype Enteritidis, and Listeria monocytogenes on Lettuce by Hydrogen-Peroxide and Lactic Acid and by Hydrogen-Peroxide with Mild Heat. Journal of Food Protection, 65, 1215-1220. https://doi.org/10.4315/0362-028X-65.8.1215
Ölmez, H. and Kretzschmar, U. (2009) Potential Alternative Disinfection Methods for Organic Fresh-Cut Industry for Minimizing Water Consumption and Environmental Impact. LWT—Food Science and Technology, 42, 686-693.
Ricke, S. (2003) Perspectives on the Use of Organic Acids and Short Chain Fatty Acids as Antimicrobials. Poultry Science, 82, 632-639. https://doi.org/10.1093/ps/82.4.632
Akbas, M. and Ölmez, H. (2007) Inactivation of Escherichia coli and Listeria monocytogenes on Iceberg Lettuce by Dip Wash Treatments with Organic Acids. Letters in Applied Microbiology, 44, 619-624. https://doi.org/10.1111/j.1472-765X.2007.02127.x
Raybaudi-Massilia, R.M., Mosqueda-Melgar, J. and Martín-Belloso, O. (2009) Antimicrobial Activity of Malic Acid against Listeria monocytogenes, Salmonella Enteritidis and Escherichia coli O157:H7 in Apple, Pear and Melon Juices. Food Control, 20, 105-112.
Ravishankar, S. and Zhu, L. (2016) Efficacy of a Citric Acid-Based Organic Sanitizer against Salmonella enterica and Background Microflora on Fresh-Cut Celery and Leeks. SDRP Journal of Food Science and Technology, 1, 1-7.
Buddhini, P., Jones, J., Ravishankar, S. and Jaroni, D. (2014) Evaluating the Efficacy of Olive, Apple and Grape Seed Extracts in Reducing Escherichia coli O157:H7 Contamination on Organic Leafy Greens during the Wash Process. International Journal of Food Science, Nutrition and Dietetics, 3, 1-7.
Lang, M.M., Harris, L.J. and Beuchat, L.R. (2004) Survival and Recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes on Lettuce and Parsley as Affected by Method of Inoculation, Time between Inoculation and Analysis, and Treatment with Chlorinated Water. Journal of Food Protection, 67, 1092-1103. https://doi.org/10.4315/0362-028X-67.6.1092
Choi, M.R., Lee, S.Y., Park, K.H., Chung, M.S., Ryu, S. and Kang, D.H. (2012) Effect of Aerosolized Malic Acid against Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157:H7 on Spinach and Lettuce. Food Control, 24, 171-176.
Litt, P.K., Ravishankar, S. and Jaroni, D. (2016) Use of Fulvic Acid Formulations as Flume-Wash Treatments for Reduction of Escherichia coli O157:H7 on Organic Leafy Greens. Food Protection Trends, 36, 284-292.
E3 Organics. (2014) Re-Usability of Chico Wash in Reducing Escherichia coli K-12 on Organic Iceberg Lettuce in a Large Scale Study. A Report Submitted to E3 Organics, Orland, CA.
King, T., Lucchini, S., Hinton, J.C. and Gobius, K. (2010) Transcriptomic Analysis of Escherichia coli O157:H7 and K-12 Cultures Exposed to Inorganic and Organic Acids in Stationary Phase Reveals Acidulant and Strain-Specific Acid Tolerance Responses. Applied and Environmental Microbiology, 76, 6514-6528. https://doi.org/10.1128/AEM.02392-09
Park, S.H., Choi, M.R., Park, J.W., Park, K.H., Chung, M.S., Ryu, S. and Kang, D.H. (2011) Use of Organic Acids to Inactivate Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on Organic Fresh Apples and Lettuce. Journal of Food Science, 76, 293-298. https://doi.org/10.1111/j.1750-3841.2011.02205.x
Doores S. (2005) Organic Acids. In: Davidson, P.M., Sofos, J.N. and Branen, A.L., Eds., Antimicrobials in Food, 3rd Edition, CRC press, Boca Raton, 91-142. https://doi.org/10.1201/9781420028737.ch4
Cole, M., Jones, M. and Holyoak, C. (1990) The Effect of pH, Salt Concentration and Temperature on the Survival and Growth of Listeria monocytogenes. Journal of Applied Bacteriology, 69, 63-72. https://doi.org/10.1111/j.1365-2672.1990.tb02912.x
Buchanan, R. and Bagi, L. (1997) Effect of Water Activity and Humectant Identity on the Growth Kinetics of Escherichia coli O157:H7. Food Microbiology, 14, 413-423. https://doi.org/10.1006/fmic.1997.0101
Moore, K.L., Patel, J., Jaroni, D., Friedman, M. and Ravishankar, S. (2011) Antimicrobial Activity of Apple, Hibiscus, Olive, and Hydrogen-Peroxide Formulations against Salmonella enterica on Organic Leafy Greens. Journal of Food Protection, 74, 1676-1683. https://doi.org/10.4315/0362-028X.JFP-11-174
Juven, B.J. and Pierson, M.D. (1996) Antibacterial Effects of Hydrogen-Peroxide and Methods for Its Detection and Quantitation. Journal of Food Protection, 59, 1233-1241. https://doi.org/10.4315/0362-028X-59.11.1233
Parish, M.E., Beuchat, L.R., Suslow, T.V., Harris, L.J., Garrett, E.H., Farber, J.N. and Busta, F.F. (2003) Methods to Reduce/Eliminate Pathogens from Fresh and Fresh-Cut Produce. Comprehensive Reviews in Food Science and Food Safety, 2, 161-173. https://doi.org/10.1111/j.1541-4337.2003.tb00033.x
Ponce, A.G., Del Valle, C.E. and Roura, S.I. (2004) Natural Essential Oils as Reducing Agents of Peroxidase Activity in Leafy Vegetables. LWT-Food Science and Technology, 37, 199-204.
Mitra, R., Cuesta-Alonso, E., Wayadande, A., Talley, J., Gilliland, S. and Fletcher, J. (2009) Effect of Route of Introduction and Host Cultivar on the Colonization, Internalization, and Movement of the Human Pathogen Escherichia coli O157: H7 in Spinach. Journal of Food Protection, 72, 1521-1530. https://doi.org/10.4315/0362-028X-72.7.1521
Macarisin, D., Patel, J., Bauchan, G., Giron, J.A. and Ravishankar, S. (2013) Effect of Spinach Cultivar and Bacterial Adherence Factors on Survival of Escherichia coli O157:H7 on Spinach Leaves. Journal of Food Protection, 76, 1829-1837. https://doi.org/10.4315/0362-028X.JFP-12-556