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Fast Fatty Acid Analysis by Core-Shell Reversed-Phase Liquid Chromatography Coupled to Evaporative Light-Scattering Detector
Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
- 1 Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
- 2 Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
- 3 Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
- 4 Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
- 5 Laboratoire Mer, Molécules, Santé (EA 2160) Le Mans Université, IUT de Laval, Laval, France
Food and Nutrition Sciences·Volume 08 (2017)·Pages 1051–1062·Published 4 December 2017·DOI10.4236/fns.2017.812077
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Abstract
A high-performance liquid chromatography analysis method with an evaporative light-scattering detector has been developed for the separation and quantitative analysis of free fatty acids in biological matrices. Core-shell reversed-phase high-performance liquid chromatography separation of 10 free fatty acids is achieved within 10.5 min using a methanol/water (0.05% trifluoroacetic acid) eluent gradient. After optimization, the drift tube and nebulization temperature of the evaporative light-scattering detector was set at 35°C, nitrogen flow-rate at 1.1 standard liter per minute and column temperature at 25°C. All calibration curves showed good regression (r 2 > 0.9975). A validation procedure following the International Conference on Harmonisation guidelines was implemented to certify the method. Relative standard deviations did not exceed 1.5% and 4.25% for repeatability and reproducibility respectively.
KeywordsFree Fatty AcidsHPLCEvaporative Light-Scattering DetectorCore-Shell TechnologyMethod Validation
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