Assessment of Physicochemical Properties, Fatty Acid Composition of Afzelia africana Seed Oil and the Effect of Its Oil-Based Diet on Body Weight and Plasma Lipid Profile of Albino Rats — Oak Academic Publishing
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Assessment of Physicochemical Properties, Fatty Acid Composition of Afzelia africana Seed Oil and the Effect of Its Oil-Based Diet on Body Weight and Plasma Lipid Profile of Albino Rats
Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
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Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
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Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
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Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
1 Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
2 Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
3 Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
4 Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria
In the present study, the physicochemical properties, fatty acid composition of Afzelia africana seed oil were determined and also effect of the oil-based diet on growth performance and plasma lipid profile were monitored using twenty Wistar albino rats allocated into two groups of ten rats each. The control group was fed with soybean-based diet while Afzelia africana oil-based diet was fed to the test group ad libitum for 28 days. Results showed the acid, saponification, iodine, and peroxide values of the oil to be 3.30 ± 0.27 mg/KOH/g oil, 124.54 ± 0.79 mg/KOH/g oil, 11.17 ± 0.39 g/100 g, and 10.01 ± 0.53 meq/g respectively. Also, linoleic (53%) and oleic acids (21.34%) were found to be the predominant unsaturated fatty acids present, while the predominant saturated fatty acid present is Palmitic acid (14.78%). The body weight of the experimental rats maintained on diet formulated with Afzelia africana seed oil was reduced compared to the rats fed with control diet. The plasma concentration of triglycerides and high density lipoprotein was not significantly different (p < 0.05) in the two groups while the total cholesterol and low density lipoprotein concentration significantly decreased in the test group fed Afzelia africana oil-based diet. The decline in plasma total cholesterol levels suggests good quality of the oil. The various parameters investigated reveal the potentials of Afzelia africana seed oil and could serve as a good diet supplement.
Keay, R.W., Onochie C.F. and Stanfield, D.P. (1964) Nigerian Trees. National Press Ltd., Forestry Research Institute of Nigeria, Ibadan, 47.
Onweluzo, J.C., Onuoha, K.C. and Obanu, Z.A. (1995) A Comparative Study of Some Functional Properties of Afzelia africana and Glycine Max Flours. Food Chemistry, 54, 55-59. https://doi.org/10.1016/0308-8146(95)92662-4
Igwenyi, I.O. and Akubugwo, E.I. (2010) Analysis of Four Seeds Used as Soup Thickeners in the South Eastern Parts of Nigeria. Kyoto International Conference Centre, Kyoto, 426-430.
Eidangbe, G.O., Ojeih, G.C., Idonije, B.O. and Oluba, O.M. (2010) Palm Oil and Egusi Melon Oil Lower Serum and Liver Lipid Profile and Improve Antioxidant in Rats Fed a High Fat Diet. Journal of Food Technology, 8, 154-158. https://doi.org/10.3923/jftech.2010.154.158
Arterburn, L.M., Oken, H.A., Bailey, H.E., Hamersley, J., Kuratko, C.N. and Hoffman, J.P (2008) Algal-Oil Capsules and Cooked Salmon: Nutritionally Equivalent Sources of Docosahexaenoic Acid. Journal of the Academy of Nutrition and Dietetics, 108, 1204-1209. https://doi.org/10.1016/j.jada.2008.04.020
Enig, M.G. (1996) Health and Nutritional Benefits from Coconut Oil: An Important Functional Food for the 21st Century. The Seminar AVOC Lauric Oils Symposium, Ho Chi Min City, 25 April 1996.
Onyechi, U.A. (1995) Potential Role of Indigenous Nigeria Food in the Treatment of Non-Insulin Dependent Diabetes Mellitus. Ph.D. Thesis, University of London, London.
Harwood, L.M. and Moody, C.J. (1989) Experimental Organic Chemistry: Principles and Practice (Illustrated Edition). Wiley-Blackwell, Hoboken, 122-125.
AOCS (2004) Official Methods and Recommended Practices of the American Oil Chemists’ Society. 5th Edition, Champaign.
Ibitoye, A.A. (2005) Laboratory Manual of Basic Methods in Analytical Chemistry. 1st Edition, Concept + IT and Educational Consult, Akure.
Friedewal, W.T., Levy, I.T. and Fredrickson, D.S. (1972) Estimation of the Concentration of Low-Density Lipoprotein Cholesterol in Plasma, without Use of Preparative Ultracentrifuge. Clinical Chemistry, 18, 499-502.
Trinder, P. (1964) Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor. Annals of Clinical Biochemistry, 6, 24-27. https://doi.org/10.1177/000456326900600108
NIHP (National Institute of Health Publication) (2001) ATP III Guidelines At-a-Glance Quick Desk Reference. National Cholesterol Education Program, No. 01-3305.
Izawa, S., Okada, M., Matsui, H. and Horita, Y.J. (1997) Quantitative Determination of HDL Cholesterol IVD. Medicine and Pharmaceutical Science, 37, 1385-1388.
Harris, J., Loftis, J.C. and Montgomery, R.H. (1987) Statistical Methods for Characterizing Ground-Water Quality. Groundwater, 25, 185-193. https://doi.org/10.1111/j.1745-6584.1987.tb02875.x
Engler, C.R. and Johnson, L.A. (1983) Effects of Processing on the Quality of Cashew Nuts. Journal of Science and Agriculture, Food Technology and Environment, 4, 105-110.
Ejikeme, P.M., Obasi, L.N. and Egbuonu, A.C. (2010) Physico-Chemical and Toxicological Studies on Afzelia africana Seed and Oil. African Journal of Biotechnology, 9, 1959-1963. https://doi.org/10.5897/AJB09.965
Ikwuagwu, O.E., Ononogbu, I.C. and Njoku, O.U. (2000) Production of Biodiesel Using Rubber Seed Oil. Industrial Crops and Products, 12, 27-63. https://doi.org/10.1016/S0926-6690(99)00068-0
Coenen, J.W. (1976) Hydrogenation of Edible Oils. Journal of the American Oil Chemists’ Society, 53, 382-389. https://doi.org/10.1007/BF02605727
Kailas, M.T., Mahajan, D.T. and Masand, V.H. (2012) Physicochemical Properties of Soybean Oil and Their Blends with Vegetable Oils for the Evaluation of Lubrication Properties. Journal of Chemical, Biological and Physical Sciences, 3, 490-497.
Amoo, I.A., Emenike, A.E. and Akpambang, V.O. (2008) Compositional Evaluation of Annonia cherimoya (Custard Apple) Fruit. Trends in Applied Sciences Research, 3, 216-220. https://doi.org/10.3923/tasr.2008.216.220
Ikhuoria, E.U. and Maliki, M. (2007) Characterisation of Avocado Pear (Persea americana) African Pear (Dacryodes edulis) Extracts. African Journal of Biotechnology, 6, 950-952.
Kochlar, S.L. (1986) Tropical Crops: A Textbook of Economic Botany. McMillian Publisher Ltd., London and Basingtone, 152-156.
Nawar, W.W. (1996) Lipids. In: Fennema, O.R., Ed., Food Chemistry, Marcel Dekker, New York, 225-314.
Marina, A.M., Che Man, Y.B. and Nazimah, S.A. (2009) Chemical Properties of Virgin Coconut Oil. Journal of American Oil Chemists’ Society, 86, 302-307. https://doi.org/10.1007/s11746-009-1351-1
SON Standard Organization of Nigeria (2000) Standard for Edible Refined Palm Oil and Its Processed Form. 2-5.
NIS Nigeria Industrial Standard (1992) Standards for Edible Vegetable Oil. 5-12.
Gan, H.L., Che Man, Y.B., Tan, C.P., NorAini, I. and Nazimah, S.A. (2005) Characterisation of Vegetable Oils by Surface Acoustic Wave Sensing Electronic Nose. Food Chemistry, 89, 507-518. https://doi.org/10.1016/j.foodchem.2004.03.005
Caterm, N.B. and Margo, A.D. (2001) Behenic Acid Is a Cholesterol-Raising Saturated Fatty Acid in Humans. American Journal of Clinical Nutrition, 73, 41-44. https://doi.org/10.1093/ajcn/73.1.41
Hunter, J.E., Zhang, J. and Kris-Etherton, P.M. (2009) Cardiovascular Disease Risk of Dietary Stearic Acid Compared with Trans, Other Saturated, and Unsaturated Fatty Acids: A Systematic Review. American Journal of Clinical Nutrition, 91, 46-63. https://doi.org/10.3945/ajcn.2009.27661
WHO (2003) Diet, Nutrition and the Prevention of Chronic Diseases. WHO Technical Report Series 916, Report of a Joint WHO/FAO Expert Consultation, World Health Organization, Geneva, 88.
Benoit, S.C., Kemp, C.J., Elias, C.F., Abplanalp, W., Herman, J.P., Migrenne, S., Lefevre, A.L., Cruciani-Guglielmacci, C., Magnan, C., Yu, F., Niswender, K., Irani, B.G., Holland, W.L. and Clegg, D.J. (2009) Palmitic Acid Mediates Hypothalamic Insulin Resistance by Altering PKC-θ Subcellular Localization in Rodents. Journal of Clinical Investigation, 119, 2577-2587. https://doi.org/10.1172/JCI36714
Pascual, G. (2016) Targeting Metastasis-Initiating Cells through the Fatty Acid Receptor CD36. Nature, 541, 41-45. https://doi.org/10.1038/nature20791
Oomah, D.B., Ladet, S., Godfrey, V.D., Liang, J. and Giarard, B. (2000) Characteristics of Raspberry (Rubus idaeus L.) Seed Oil. Food Chemistry, 69, 187-193. https://doi.org/10.1016/S0308-8146(99)00260-5
USDA (2016) Nutrient Data Laboratory Home Page. USDA National Nutrient Database for Standard Reference, Release 20. U.S. Department of Agriculture, Agricultural Research Service.
Rudel, L.L., Kelly, K., Sawyer, J.K., Shah, R. and Wilso, M.D. (1998) Dietary Monounsaturated Fatty Acids Promote Aorti Atherosclerosis in LDL Receptor-Null ApoB100-Overexpressing Transgenic Mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 18, 1818-1827. https://doi.org/10.1161/01.ATV.18.11.1818
Ruggeri, S., Cappelloni, M., Gambelli, L., Nicoli, S. and Carnovale, E. (1998) Chemical Composition and Nutritive Value of Nuts Grown in Italy. Italian Journal of Food Science, 10, 243-252.
Corbett, P. (2003) It Is Time for an Oil Change! Opportunities for High Oleic Vegetables Oils. Inform, 14, 480-481.
Worthington, R.E. and Hammons, R.O. (1977) Variability in Fatty Acid Composition among Arachis Genotypes: A Potential Source of Product Improvement. Journal of the American Oil Chemists’ Society, 54, 105-108. https://doi.org/10.1007/BF02912383
Sanders, T.H., Lansden, J.A., Greene, R.L., Drexler, J.S. and Williams, E.J. (1992) Oil Characteristics of Peanut Fruit Separated by a Nondestructive Maturity Classification Method. Peanut Science, 9, 20-23. https://doi.org/10.3146/i0095-3679-9-1-6
Feed Conversion Ratio (FCR) (2011) USAID Technical Bulletin. http://www.fintrac.com
Fleming, S.E. and Lee, B. (1983) Growth Performance and Intestinal Transit Time of Rats Fed Purified and Natural Dietary Fibres. The Journal of Nutrition, 133, 592-601. https://doi.org/10.1093/jn/113.3.592
Farran, M.T., Halaby, W.S., Barbour, G.W., Uwayjan, M.G., Sleiman, F.T. and Ashkarian, V.M. (2005) Effects of Feeding Ervil (Vicia ervilia) Seeds Soaked in Water or Acetic Acid on Performance and Internal Organ Size of Broilers and Production and Egg Quality of Laying Hens. Poultry Science, 84, 1723-1728. https://doi.org/10.1093/ps/84.11.1723
Lawn, R.M. (1992) Lipoprotein A in Heart Disease. Scientific American, 262, 26-32. https://doi.org/10.1038/scientificamerican0692-54
Adedeji, A.L., Akintola, A.O., Oyewopo, A.O., Omotoso, E.O. and Badmus, J.A. (2008) Effects of Three Nigerian Stable Oils on the Plasma Lipid Profile of Wistar Rats. Research Journal of Medical Sciences, 2, 193-196.
Getz, G.S. and Reardon, C.A. (2006) Diet and Murine Atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 26, 242-249. https://doi.org/10.1161/01.ATV.0000201071.49029.17
Ohtani, H., Hayashi, K., Hirata, Y., Dojo, S., Nakashima, K., Nishio, E., Kurushima, H., Saeki, M. and Kajiyama, G. (1990) Effects of Dietary Cholesterol and Fatty Acids on Plasma Cholesterol Level and Hepatic Lipoprotein Metabolism. The Journal of Lipid Research, 31, 1413-1422.
Tishinsky, J.M. (2013) Modulation of Adipokines by n-3 Polyunsaturated Fatty Acids and Ensuing Changes in Skeletal Muscle Metabolic Response and Inflammation. Applied Physiology, Nutrition, and Metabolism, 38, 361. https://doi.org/10.1139/apnm-2012-0447
Choi, Y., Ahn, C., Rhee, H., Choe, M., Kim, C. and Kim, J. (1993) Comparative Effects of Dietary Palm Oil, Perilla Oil, and Soybean Oil on Lipid Profiles in Differently Aged Rats Fed on Hypercholesterolemic Diets. Bioscience, Biotechnology, and Biochemistry, 57, 65-68. https://doi.org/10.1271/bbb.57.65
Ramachandran, D.H., Narasimhamurthy, K. and Raina, P.L. (2003) Modulation of Cholesterol Induced Hypercholesterolemia through Dietary Factors in Indian Desert Gerbils (Meriones hurrianae). Nutrition Research, 23, 245-256. https://doi.org/10.1016/S0271-5317(02)00517-1
Almario, R.U., Vonghavaravat, V., Wong, R. and Kasim-Karakas, S.E. (2001) Effects of Walnut Consumption on Plasma Fatty Acids and Lipoproteins in Combined Hyperlipidemia. American Journal of Clinical Nutrition, 74, 71-79. https://doi.org/10.1093/ajcn/74.1.72
Fashing, P., Ratheiser, K., Waldhausl, W., Rohac, M., Osterrode, W., Nowotny, P. and Vierphapper, H. (1991) Metabolic Effects of Fish-Oil in Patients with Impaired Glucose Tolerance. Diabetes, 40, 583-589. https://doi.org/10.2337/diab.40.5.583
Lichtenstein, A.H., Matthan, N.R., Jalbert, S.M., Resteghini, N.A., Schaefer, E.J. and Ausman, L.M. (2006) Novel Soybean Oils with Different Fatty Acid Profiles Alter Cardiovascular Disease Risk Factors in Moderately Hyperlipidemic Subjects. The American Journal of Clinical Nutrition, 84, 497-504. https://doi.org/10.1093/ajcn/84.3.497
Babalola, T.O. and Apata, D.F. (2011) Chemical and Quality Evaluation of Some Alternative Lipid Sources for Aqua Feed Production. Agriculture and Biology Journal of North America, 2, 935-943. https://doi.org/10.5251/abjna.2011.2.6.935.943
Sena, C.M., Nunes, E., Louro, T., Proenca, T., Fernandes, R., Boarder, M.R. and Seica, R.M. (2008) Effects of α-Lipoic Acid on Endothelial Function in Aged Diabetic and High-Fat Fed Rats. British Journal of Pharmacology, 153, 894-906. https://doi.org/10.1038/sj.bjp.0707474