Sugar fatty acid esters (SFAE) are a class of synthetic emulsifiers used in the food, pharmaceutical, and personal care industries. The influence of the fatty acid chain length on the emulsification properties of lactose fatty acid esters (LFAE) including lactose monooctanoate (LMO), lactose monodecanoate (LMD), lactose monolaurate (LML) and lactose monomyristate (LMM) was investigated in this study. The stability of the emulsions as well as the oil droplet size distribution in 20% soybean oil-in-water emulsions was measured at 0.1%, 0.25% and 0.5% of LFAE concentrations. In order of LFAE with the strongest emulsion stabilization characteristics were LML, LMD, LMO and LMM. Oil droplet distributions resulted in the same trend, with LML and LMD maintaining the smallest droplet sizes and thus the most stable emulsion. The hydrophilic-lipophilic balance (HLB) and critical micelle concentrations were determined for each LFAE. An increase in HLB value was seen with an increased CMC value for each LFAE, showing the strength of the linear relationship between these two measured values. Additionally, there was a decrease in HLB and CMC values with a decrease in the fatty acid chain length of each LFAE. This research showed that LML and LMD formed more stable emulsions, even with HLB and CMC values higher than those of LMM suggesting HLB and CMC values alone do not predict emulsifier effectiveness.
Zhang, X., Song, F., Taxipalati, M., Wei, W. and Feng, F. (2014) Comparative Study of Surface-Active Properties and Antimicrobial Activities of Disaccharide Monoesters. PLoS ONE, 9, e114845. https://doi.org/10.1371/journal.pone.0114845
Becerra, N., Toro, C., Zanocco, A.L., Lemp, E. and Günther, G. (2008) Characterization of Micelles Formed by Sucrose 6-O-Monoesters. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 327, 134-139. https://doi.org/10.1016/j.colsurfa.2008.06.012
Yanke, L., Shufen, Z., Qinghui, W. and Jinzong, Y. (2004) Relationship of Solubility Parameters to Interfacial Properties of Sucrose Esters. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 248, 127-133. https://doi.org/10.1016/j.colsurfa.2004.07.032
Piao, J. and Adachi, S. (2006) Stability of O/W Emulsions Prepared Using Various Monoacyl Sugar Alcohols as an Emulsifier. Innovative Food Science & Emerging Technologies, 7, 211-216. https://doi.org/10.1016/j.ifset.2006.04.002
Soultani, S., Ognier, S., Engasser, J.-M. and Ghoul, M. (2003) Comparative Study of Some Surface Active Properties of Fructose Esters and Commercial Sucrose Esters. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 227, 35-44. https://doi.org/10.1016/S0927-7757(03)00360-1
Ferrer, M., Comelles, F., Plou, F.J., Cruces, M.A., Fuentes, G., Parra, J.L. and Ballesteros, A. (2002) Comparative Surface Activities of Di- and Trisaccharide Fatty Acid Esters. Langmuir, 18, 667-673. https://doi.org/10.1021/la010727g
Garofalakis, G., Murray, B.S. and Sarney, D.B. (2000) Surface Activity and Critical Aggregation Concentration of Pure Sugar Esters with Different Sugar Headgroups. Journal of Colloid Interface Science, 229, 391-398. https://doi.org/10.1006/jcis.2000.7035
Walsh, M.K., Bombyk, R.A., Wagh, A., Bingham, A. and Berreau, L.M. (2009) Synthesis of Lactose Monolaurate as Influenced by Various Lipases and Solvents. Journal of Molecular Catalysis B: Enzymatic, 60, 171-177. https://doi.org/10.1016/j.molcatb.2009.05.003
do Neta, N.A.S., Santos, J.C.S., de Sancho, S.O., Rodrigues, S., Goncalves, L.R.B., Rodrigues, L.R. and Teixeira, J.A. (2012) Enzymatic Synthesis of Sugar Esters and Their Potential as Surface-Active Stabilizers of Coconut Milk Emulsions. Food Hydrocolloids, 27, 324-331. https://doi.org/10.1016/j.foodhyd.2011.10.009
Chiralt, A. (2005) Food Emulsions. Food Engineering, Vol II. Oxford Press, Oxford. http://www.eolss.net/sample-chapters/c10/E5-10-03-05.pdf
Stability of Various Beverage Emulsions (2009) Food Application Paper. 1-7. http://www.titanex.com.tw/doc/tecsupport/ANF-Turbiscan-application%20paper%20on%20%20 beverage%20emulsions.pdf
Bai, L. and McClements, D.J. (2016) Extending Emulsion Functionality: Post-Homogenization Modification of Droplet Properties. Processes, 4, 2-18. https://doi.org/10.3390/pr4020017
Trujillo-Cayado, L.A., Natera, A., García, M.C., Munoz, J. and Alfaro, M.C. (2015) Rheological Properties and Physical Stability of Ecological Emulsions Stabilized by a Surfactant Derived from Cocoa Oil and High Pressure Homogenization. Grasas y Aceites, 66, e087. https://doi.org/10.3989/gya.1313143
Kaombe, D.D., Lenes, M., Toven, K. and Glomm, W.R. (3013) Turbiscan as a Tool for Studying the Phase Separation Tendency of Pyrolysis Oil. Energy & Fuels, 27, 1446-1452. https://doi.org/10.1021/ef302121r
Huck-Iriart, C., Candal, R.J. and Herrera, M.L. (2011) Effect of Processing Conditions and Composition on Sodium Caseinate Emulsions Stability. Procedia Food Science, 1, 116-122. https://doi.org/10.1016/j.profoo.2011.09.019
Garg, N., Martini, S., Britt, D.W. and Walsh, M.K. (2010) Emulsifying Properties of Lactose-Amines in Oil-in-Water Emulsions. Food Research International, 43, 1111-1115. https://doi.org/10.1016/j.foodres.2010.02.003
Beckman Coulter (2011) Instruction for Use LS 13 320 Laser Diffraction Particle Size Analyzer. Beckman Coulter Inc. https://www.beckmancoulter.com/wsrportal/techdocs?docname=B05577AB.pdf
Szuts, A. and Szabó-Révész, P. (2012) Sucrose Esters as Natural Surfactants in Drug Delivery Systems—A Mini-Review. International Journal of Pharmaceutics, 433, 1-9.
Patist, A., Bhagwat, S.S., Penfield, K.W., Aikens, P. and Shah, D.O. (2000) On the Measurement of Critical Micelle Concentrations of Pure and Technical-Grade Nonionic Surfactants. Journal of Surfactants and Detergents, 3, 53-58. https://doi.org/10.1007/s11743-000-0113-4
Suradkar, Y.R. and Bhagwat, S.S. (2006) CMC Determination of an Odd Carbon Chain Surfactant (C13 E20) Mixed with Other Surfactants Using a Spectrophotometric Technique. Journal of Chemical & Engineering Data, 51, 2026-2031. https://doi.org/10.1021/je060064a
Hait, S.K. and Moulik, S.P. (2001) Determination of Critical Micelle Concentration (CMC) of Nonionic Surfactants by Donor-Acceptor Interaction with Iodine and Correlation of CMC with Hydrophile-Lipophile Balance and Other Parameters of the Surfactants. Journal of Surfactants and Detergents, 4, 303-309. https://doi.org/10.1007/s11743-001-0184-2
Lee, S.-M., Sandhu, G. and Walsh, M.K. (2017) Growth Inhibitory Properties of Lactose Fatty Acid Esters. Saudi Journal of Biological Sciences, 24, 1483-1488. https://doi.org/10.1016/j.sjbs.2015.10.013
Wagh, A., Shen, S., Shen, F.A., Miller, C.D. and Walsh, M.K. (2012) Investigating the Effect of Lactose Monolaurate on Pathogenic and Non-Pathogenic Bacteria. Applied Environmental Microbiology, 78, 3465-3468. https://doi.org/10.1128/AEM.07701-11
Chen, Y., Nummer, B. and Walsh, M.K. (2014) Anti-Listerial Activity of Lactose Monolaurate in Milk, Drinkable Yogurt and Cottage Cheese. Letters in Applied Microbiology, 58, 156-162. https://doi.org/10.1111/lam.12169
El Rassi, Z. (1995) Carbohydrate Analysis: High Performance Liquid Chromatography and Capillary Electrophoresis. Elsevier, Amsterdam, 672.
Ritthitham, S. (2009) Synthesis of Sucrose Fatty Acid Esters as Catalyzed by Alkaline Protease AL 89 and Candida antarctica Lipase B in Hydrophilic Solvents. Department of Chemistry and Bioscience, Aalborg University, Aalborg.
Tadros, T.F. (2013) Emulsion Formation and Stability. Wiley Online Library.
Smith, J. (1991) Food Additive User’s Handbook. Springer, Berlin.
Pawlik, A., Kurukji, D., Norton, I. and Spyropoulos, F. (2016) Food-Grade Pickering Emulsions Stabilised with Solid Lipid Particles. Food & Function, 7, 2712-2721. https://doi.org/10.1039/C6FO00238B
Giulietti, M. and Bernardo, A. (2012) Crystallization by Antisolvent Addition and Cooling, Crystallization—Science and Technology. InTech, London.
Rao, J. and McClements, D.J. (2011) Food-Grade Microemulsions, Nanoemulsions and Emulsions: Fabrication from Sucrose Monopalmitate & Lemon Oil. Food Hydrocolloids, 25, 1413-1423. https://doi.org/10.1016/j.foodhyd.2011.02.004