Para-probiotics are ghost probiotics and consist of non-viable microbial cells. Here, a genus of acetic acid bacteria (AAB) was lyophilized to produce a para-probiotic preparation of non-viable (NV) AAB. The NV-AAB orally administered for 49 days ameliorated antigen-induced sneezing and plasma antigen-specific IgE in an ovalbumin-sensitized allergic mouse model. Our findings indicated that NV-AAB, as a para-probiotic functional food, exhibits an anti-allergic effect. The anti-allergic mechanism of NV-AAB was atypical because of the near-absence of an effect on Th1 and Th2 balance in splenocytes. NV-AAB will be a unique para-probiotic food material with an anti-allergic activity.
KeywordsProbioticsDead CellFunctional FoodTregIgE
Isolauri, E., Sütas, Y., Kankaanpää, P., Arvilommi, H. and Salminen, S. (2001) Probiotics: Effects on Immunity. The American Journal of Clinical Nutrition, 73, 444S-450S. https://doi.org/10.1093/ajcn/73.2.444s
Adams, C.A. (2010) The Probiotic Paradox: Live and Dead Cells Are Biological Response Modifiers. Nutrition Research Reviews, 23, 37-46. https://doi.org/10.1017/S0954422410000090
Lahtinen, S.J. (2012) Probiotic Viability: Does It Matter? Microbial Ecology in Health and Disease, 18, 23.
Nighswonger, B.D., Brashears, M.M. and Gilliland, S.E. (1996) Viability of Lactobacillus Acidophilus and Lactobacillus Casei in Fermented Milk Products during Refrigerated Storage. Journal of Dairy Science, 79, 212-219. https://doi.org/10.3168/jds.S0022-0302(96)76353-1
Dotan, I. and Rachmilewitz, D. (2005) Probiotics in Inflammatory Bowel Disease: Possible Mechanisms of Action. Current Opinion in Gastroenterology, 21, 426-430.
Sashihara, T., Sueki, N. and Ikegami, S. (2006) An Analysis of the Effectiveness of Heat-Killed Lactic Acid Bacteria in Alleviating Allergic Diseases. Journal of Dairy Science, 89, 2846-2855. https://doi.org/10.3168/jds.S0022-0302(06)72557-7
Zhang, L., Li, N., Caicedo, R. and Neu, J. (2005) Alive and Dead Lactobacillus Rhamnosus GG Decrease Tumor Necrosis Factor-Alpha-Induced Interleukin-8 Production in Caco-2 Cells. The Journal of Nutrition, 135, 1752-1756. https://doi.org/10.1093/jn/135.7.175
Kimoto-Nira, H., Mizumachi, K., Okamoto, T., Sasaki, K. and Kurisaki, J. (2009) Influence of Long-Term Consumption of a Lactococcus Lactis Strain on the Intestinal Immunity and Iintestinal Flora of the Senescence-Accelerated Mouse. The British Journal of Nutrition, 102, 181-185. https://doi.org/10.1017/S0007114508143574
Moroi, M., Uchi, S., Nakamura, K., Sato, S., Shimizu, N., Fujii, M., et al. (2011) Beneficial Effect of a Diet Containing Heat-Killed Lactobacillus Paracasei K71 on Adult Type Atopic Dermatitis. The Journal of Dermatology, 38, 131-139. https://doi.org/10.1111/j.1346-8138.2010.00939.x
Lopez, M., Li, N., Kataria, J., Russell, M. and Neu, J. (2008) Live and Ultraviolet-Inactivated Lactobacillus Rhamnosus GG Decrease Flagellin-Induced Interleukin-8 Production in Caco-2 Cells. The Journal of Nutrition, 138, 2264-2268. https://doi.org/10.3945/jn.108.093658
Guglielmetti, S., Tamagnini, I., Mora, D., Minuzzo, M., Scarafoni, A., Arioli, S., et al. (2008) Implication of an Outer Surface Lipoprotein in Ahesion of Bifidobacterium Bifidum to Caco-2 Cells. Applied and Environmental Microbiology, 74, 4695-4702. https://doi.org/10.1128/AEM.00124-08
Taverniti, V. and Guglielmetti, S. (2011) The Immunomodulatory Properties of Probiotic Microorganisms beyond Their Viability (Ghost Probiotics: Proposal of Paraprobiotic Concept). Genes & Nutrition, 6, 261-274. https://doi.org/10.1007/s12263-011-0218-x
de Almada, C.N., Almada, C.N., Martinez, R.C.R. and Sant’Ana, A.S. (2016) Paraprobiotics: Evidences on Their Ability to Modify Biological Responses, Inactivation Methods and Perspectives on Their Application in Foods. Trends in Food Science & Technology, 58, 96-114. https://doi.org/10.1016/j.tifs.2016.09.011
Lee, M.Y., Ahn, K.S., Kwon, O.K., Kim, M.J., Kim, M.K., Lee, I.Y., et al. (2007) Anti-Inflammatory and Anti-Allergic Effects of Kefir in a Mouse Asthma Model. Immunobiology, 212, 647-654. https://doi.org/10.1016/j.imbio.2007.05.004
Daneshmandi, S., Hajimoradi, M., Soleimani, N. and Sattari, M. (2011) Modulatory Effect of Acetobacter Xylinum Cellulose on Peritoneal Macrophages. Immunopharmacology and Immunotoxicology, 33, 164-168. https://doi.org/10.3109/08923973.2010.491080
Amano, S., Inagawa, H., Nakata, Y., Ohmori, M., Kohchi, C. and Soma, G. (2015) Oral Administration of Lipopolysaccharide of Acetic Acid Bacteria Protects Pollen Allergy in a Murine Model. Anticancer Research, 35, 4509-4514.
Ishii, Y. (2009) Perspective of Prevention and Treatment for Allergic Diseases. Seikagaku, 81, 209-217. (In Japanese)
Dash, G., Raman, R.P., Pani Prasad, K., Makesh, M., Pradeep, M.A. and Sen, S. (2015) Evaluation of Paraprobiotic Applicability of Lactobacillus Plantarum in Improving the Immune Response and Disease Protection in Giant Freshwater Prawn, Macrobrachium rosenbergii (de Man, 1879). Fish & Shellfish Immunology, 46, 167-174. https://doi.org/10.1016/j.fsi.2014.12.007
Kanauchi, O., Andoh, A., AbuBakar, S. and Yamamoto, N. (2018) Probiotics and Paraprobiotics in Viral Infection: Clinical Application and Effects on the Innate and Acquired Immune Systems. Current Pharmaceutical Design, 24, 710-717. https://doi.org/10.2174/1381612824666180116163411
Palomares, O., Martín-Fontecha, M., Lauener, R., Traidl-Hoffmann, C., Cavkaytar, O., Akdis, M., et al. (2014) Regulatory T Cells and Immune Regulation of Allergic Diseases: Roles of IL-10 and TGF-β. Genes and Immunity, 15, 511-520. https://doi.org/10.1038/gene.2014.45
Cottrez, F., Hurst, S.D., Coffman, R.L. and Groux, H. (2000) T Regulatory Cells 1 Inhibit a Th2-Specific Response in Vivo. Journal of Immunology, 165, 4848-4853. https://doi.org/10.4049/jimmunol.165.9.4848
Yamamoto, K., Kawamura, I., Tominaga, T., Nomura, T., Ito, J. and Mitsuyama, M. (2006) Listeriolysin O Derived from Listeria Monocytogenes Inhibits the Effector Phase of an Experimental Allergic Rhinitis Induced by Ovalbumin in Mice. Clinical and Experimental Immunology, 144, 475-484. https://doi.org/10.1111/j.1365-2249.2006.03092.x
Atarashi, K., Tanoue, T., Shima, T., Imaoka, A., Kuwahara, T., Momose, Y., et al. (2011) Induction of Colonic Regulatory T Cells by Indigenous Clostridium Species. Science, 331, 337-341. https://doi.org/10.1126/science.1198469
Smith, P.M., Howitt, M.R., Panikov, N., Michaud, M., Gallini, C.A., Bohlooly, Y.M., et al. (2013) The Microbial Metabolites, Short-Chain Fatty Acids, Regulate Colonic Treg Cell Homeostasis. Science, 341, 569-573. https://doi.org/10.1126/science.1241165
Geuking, M.B., McCoy, K.D. and Macpherson, A.J. (2013) Metabolites from Intestinal Microbes Shape Treg. Cell Research, 23, 1339-1340. https://doi.org/10.1038/cr.2013.125
Stock, P., Akbari, O., Berry, G., Freeman, G.J., Dekruyff, R.H. and Umetsu, D.T. (2004) Induction of T Helper Type 1-Like Regulatory Cells That Express Foxp3 and Protect against Airway Hyper-Reactivity. Nature Immunology, 5, 1149-1156. https://doi.org/10.1038/ni1122