This study investigated the use of a product based on yacon (PBY) in microbiological, physical-chemical and intestinal characteristics of Wistar rats artificially constipated with Loperamide ® . Thirty-two rats were divided into four groups: Control (C), Constipated Control (CC), PBY (not constipated) and Constipated PBY (PBYC). The dosage of 0.14 g of FOS+ inulin/kg was tested. Microbiota, pH and faeces characteristics of faeces and caecal contents were evaluated. Caecal weight, morphometry of caecal villi and the concentration of short-chain fatty acids were determined. Higher caecal weight was identified in the PBYC animals as well as higher width, height and depth of cripts. The PBY group showed the highest (p < 0.05) concentration of butyrate (93.2 ± 65.5 mmol/L). The supplementation with PBY positively altered the intestine epithelial tissue in constipated animals, keeping the integrity of the caecum crypts.
Waitzberg, D.L., Logullo, L.C., Bittencourt, A.F., Torrinhas, R.S., Shirom, G.M., Paulino, N.P., et al. (2013) Effect of Synbiotic in Constipated Adult Women: A Randomized, Double-Blind, Placebo-Controlled Study of Clinical Response. Clinical Nutrition, 32, 27-33. https://doi.org/10.1016/j.clnu.2012.08.010
Louis, P., Scott, K.P., Duncan, S.H. and Flint, H.J. (2007) Understanding the Effects of Diet on Bacterial Metabolism in the Large Intestine. Journal of Applied Microbiology, 102, 1197-1208. https://doi.org/10.1111/j.1365-2672.2007.03322.x
Zhao, Y. and Yu, Y. (2016) Intestinal Microbiota and Chronic Constipation. Springer Plus, 5, 1130. https://doi.org/10.1186/s40064-016-2821-1 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951383/pdf/40064_2016_Article_2821.pdf
Manhart, N., Spittler, A., Bergmeister, H., Mittlbock, M. and Roth, E. (2003) Influence of Fructooligosaccharides on Peyer’s Patch Lymphocyte Numbers in Healthy and Endotoxemic Mice. Nutrition, 19, 657-660. https://doi.org/10.1016/S0899-9007(03)00059-5
Scholz-Ahrens, K.E., Ade, P., Marten, B., Weber, P., Timm, W., Asil, Y., Gluer, C.C. and Schrezenmeir, J. (2007) Prebiotics, Probiotics, and Synbiotics Affect Mineral Absorption, Bone Mineral Content, and Bone Structure. Journal of Nutrition, 137, 838S-846S. https://doi.org/10.1093/jn/137.3.838S
Cherbut, C., Michel, C. and Lecannu, G. (2004) The Prebiotic Characteristics of Fructooligosaccharides Are Necessary Forreduction of TNBS-Induced Colitis in Rats. The Journal of Nutrition, 133, 21-27. https://doi.org/10.1093/jn/133.1.21
Barbara, G., Stanghellini, V., Brandi, G., Cremon, C., Di Nardo, G., De Giorgio, R., et al. (2005) Interactions between Commensal Bacteria and Gut Sensorimotor Function in Health and Disease. American Journal of Gastroenterology, 100, 2560-2568. https://doi.org/10.1111/j.1572-0241.2005.00230.x
Gibson, G. and Roberfroid, M. (1995) Dietary Modulation of the Human Colonic Microbiota Introducing the Concept of Prebiotics. Journal of Nutrition, 125, 1401-1412.10.
Gibson, G.R., Hutkins, R., Sanders, M.E., Prescott, S.L., Remmer, R.A., Salminen, S.J., Scott, K., Stanton, C., Swanson, K.S., Cami, P.D., Verbeke, K. and Reid, G. (2017) The International Scientific Association for Probiotics and Prebiotics (ISAPP) Consensus Statement on the Definition and Scope of Prebiotics. Expert Consensus Document. Nature Ver./Gastroenterol & Hepatol., 14, 491-502. https://doi.org/10.1038/nrgastro.2017.75 https://www.nature.com/articles/nrgastro.2017.75.pdf
Lobo, A., Colli, C., Eliana, P. and Tullia, M. (2007) Effects of Fructans Containing Yacon [Smallanthus sonchifolius Poepp & Endl.] Flour on Caecum Mucosal Morphometry, Calcium and Magnesium Balance, and Bone Calcium Retention in Growing Rats. Britsh Journal of Nutrition, 97, 776-785. https://doi.org/10.1017/S0007114507336805
Passos, L.M.L. and Park, Y.K. (2003) Frutooligossacarídeos: Implicaçoes na saúde humana e utilizaçao em alimentos. Ciencia Rural, 33, 385-390. https://doi.org/10.1590/S0103-84782003000200034
Fortes, R.C. and Muniz, L.B. (2009) Efeitos da suplementaçao dietética com frutooligossacarídeos e inulina no organismo humano: Estudo baseado em evidencias. Comunicaçao em Ciencias da Saúde, 20, 241-252.
Aybar, M., Sanchez-Riera, A., Grau, A. and Sanchez, S. (2001) Hypoglycemic Effect of the Water Extracts of Smallantus sonchifolius [yacon] Leaves in Normal and Diabetic Rats. Journal of Ethnopharmacology, 74, 125-132. https://doi.org/10.1016/S0378-8741(00)00351-2
Graefe, S., Hermann, M., Manrique, I., Golombek, S. and Buerkert, A. (2004) Effects of Post-Harvest Treatments on the Carbohydrate Composition of Yacon Roots in the Peruvian Andes. Field Crops Research, 86, 157-165. https://doi.org/10.1016/j.fcr.2003.08.003
Vilhena, S.M.C., Camara, F.L.A. and Kakihara, S.T. (2000) O cultivo de yacon no Brasil. Horticultura Brasileira, 18, 5-8. https://doi.org/10.1590/S0102-05362000000100002
Lachman, J., Fernandez, E.C. and Orsak, M. (2003) Yacon [Smallanthus sonchifolia (Poepp. et Endl.) H. Robinson] Chemical Composition and Use—A Review. Plant, Soil and Environment, 49, 283-290. https://doi.org/10.17221/4126-PSE
Genta, S., Cabrera, W., Habib, N., Pons, J., Carillo, I.M., Grau, A. and Sánchez, S. (2009) Yacon Syrup: Beneficial Effects on Obesity and Insulin Resistance in Humans. Clinical Nutrition, 28, 182-187. https://doi.org/10.1016/j.clnu.2009.01.013
Rodrigues, V.C. (2011) Formulaçao, índice glicemico e aplicaçao alimentar de um produto à base de yacon [Smallanthus sonchifolius]. Tesis, 89 p.
Scheid, M.M.A., Genaro, O.S., Moreno, Y.M.F. and Pastore, G.M. (2014) Freeze Dried Powdered Yacon: Effects of FOS on Serum Glucose, Lipids and Intestinal Transit in the Elderly. European Journal of Nutrition, 53, 1457-1464. https://doi.org/10.1007/s00394-013-0648-x
Moura, N.A., Caetano, B.F.R., Sivieri, K., Urbano, L.H., Cabello, C., Rodrigues, M.A.M. and Barbisan, L.F. (2012) Protective Effects of Yacon (Smallanthus sonchifolius) Intake on Experimental Colon Carcinogenesis. Food and Chemical Toxicology, 50, 2902-2910. https://doi.org/10.1016/j.fct.2012.05.006
Geyer, M., Manrique, I., Degen, L. and Beglinger, C. (2008) Effect of Yacon [Smallanthus sonchifolius] on Colonic Transit Time in Healthy Volunteers. Digestion, 78, 30-33. https://doi.org/10.1159/000155214
Pare, P., Ferrazzi, S., Thompson, W.G., Irvine, E.J. and Rance, L. (2001) An Epidemiological Survey of Constipation in Canada: Definitions, Rates, Demographics, and Predictors of Health Care Seeking. The American Journal of Gastroenterology, 96, 3130-3137. https://doi.org/10.1111/j.1572-0241.2001.05259.x
Higgins, P.D. and Johanson, J.F. (2004) Epidemiology of Constipation in North America: A Systematic Review. The American Journal of Gastroenterology, 99, 750-759. https://doi.org/10.1111/j.1572-0241.2004.04114.x
Sant’Anna, M.S.L., Coelho, C.G. and Ferreira, C.L.L.F. (2012) Prevalencia de constipaçao intestinal no município de Viçosa-MG. 10th Congresso Brasileiro de Saúde Coletiva, Porto Alegre, 14 November 2012, 134-135.
Kaur, N. and Gupta, A.K. (2002) Applications of Inulin and Oligofructose in Health and Nutrition. Journal of Biosciences, 27, 703-714. https://doi.org/10.1007/BF02708379
Bielecka, M., Biedrzycka, E., Majkowska, A., Juskiewicz, J. and Wróblewska, M. (2002) Effect of Non-Digestible Oligosaccharides on Gut Microecosystem in Rats. Food Research International, 35, 139-144. https://doi.org/10.1016/S0963-9969(01)00175-2
Association of Official Analytical Chemists (1997) Official Methods of Analysis of the AOAC. 12th Edition, Washington DC. https://doi.org/10.11606/D.10.2003.tde-31052004-092206
Shimotoyodome, A., Meguro, S., Hase, T., Tokimitsu, I. and Sakata, T. (2000) Decreased Colonic Mucus in Rats with Loperamide-Induced Constipation. Comparative Biochemistry and Physiology, 126, 203-221. https://doi.org/10.1016/S1095-6433(00)00194-X
Veronesi, C. (2003) Efeito de dois alimentos comerciais secos no consumo energético, peso vivo e peso metabólico, escore corporal, escore e peso fecal de caes adultos em manutençao e atividade. 93f. Dissertaçao, USP.
Rogosa, M., Mitchell, J.A. and Wiseman, R.F. (1951) A Selective Medium for the Isolation and Enumeration of Oral and Fecal Lactobacilli. Journal of Bacteriology, 62, 132-133.
Hartemink, R., Vanlaere, K.M.J. and Rombouts, F.M. (1997) Growth of Enterobacteria on Fructooligosaccharides, Wageningnen, Holanda. Journal of Applied Microbiology, 383, 367-374. https://doi.org/10.1046/j.1365-2672.1997.00239.x
Santos, M.S., Ferreira, C.L.L.F., Santos, J.L., Pozza, P.C. and Teshima, E. (2003) Influencia do fornecimento de probiótico à base de Lactobacillus sp. sobre a microbiota intestinal de leitoes. Ciencia e Agrotecnologia, 27, 1395-1400. https://doi.org/10.1590/S1413-70542003000600026
Rosa, D.D., Sales, R.L., Moraes, L.F.S., Lourenço, F.C., Neves, C.A., Sabarense, C.M. and Pelúzio, M.C.G. (2010) Flaxseed, Olive and Fish Oil Influence Plasmatic Lipids, Lymphocyte Migration and Morphometry of the Intestinal of Wistar Rats. Acta Cirurgica Brasileira, 25, 275-280. https://doi.org/10.1590/S0102-86502010000300010
Smirick-Ktjardes, M.R., Griedshop, C.M. and Flickinger, E.A. (2003) Dietary Galactooligosaccharides Affect Ileal and Total-Tract Nutrient Digestibility, Ileal and Fecal Bacterial Concentrations, and Ileal Fermentive Characteristics of Growing Pigs. American Society of Animal Science, 81, 2535-2545.
Takasaki, K., Kishibayashi, N., Ishii, A. and Kaarasawa, A. (1994) Effects of KW-5092, a Novel Gastroprokinetic Agent, on the Delayed Colonic Propulsion in Rats. The Japanese Journal of Pharmacology, 65, 67-71. https://www.ncbi.nlm.nih.gov/pubmed/8089932
Campbell, J.M., Fahey, G.C. and Wolf, B.W. (1997) Selected Indigestible Oligosaccharides Affect Large Bowel Mass, Cecal and Fecal Short-Chain Fatty Acids, ph and Microflora in Rats. Journal of Nutrition, 127, 130-136. https://doi.org/10.1093/jn/127.1.130
Lucke, K., Miehlke, S., Jacobs, E. and Schuppler, M. (2006) Prevalence of Bacteroides and Prevotella spp. in Ulcerative Colitis. Journal of Medical Microbiology, 55, 617-624. https://doi.org/10.1099/jmm.0.46198-0
Montesi, A., Garcia-Albiach, R., Pozuelo, M.J., Pintado, C., Goni, I. and Rotger, R. (2005) Molecular and Microbiological Analysis of Caecal Microbiota in Rats Fed with Diets Supplemented Either with Prebiotics or Probiotics. International Journal of Food Microbiology, 98, 281-289. https://doi.org/10.1016/j.ijfoodmicro.2004.06.005
Rodriguez-Cabezas, M.E., Camuesco, D., Arribas, B., Garrido-Mesa, N., Comalada, M., Bailón, E., et al. (2010) The Combination of Fructooligosaccharides and Resistant Starch Shows Prebiotic Additive Effects in Rats. Clinical Nutrition, 29, 832-839. https://doi.org/10.1016/j.clnu.2010.05.005
Hernot, D.C., Boileau, T.W., Bauer, L.L., Middelbos, I.S., Murphy, M.R., Swanson, K.S., et al. (2009) In Vitro Fermentation Profiles, Gas Production Rates, and Microbiota Modulation as Affected by Certain Fructans, Galactooligosaccharides, and Polydextrose. Journal of Agricultural and Food Chemistry, 57, 1354-1361. https://doi.org/10.1021/jf802484j
Van de Wiele, T., Boon, N., Possemiers, S., Jacobs, H. and Verstraete, W. (2007) Inulin-Type Fructans of Longer Degree of Polymerization Exert More Pronounced in Vitro Prebiotic Effects. Journal of Applied Microbiology, 102, 452-460. https://doi.org/10.1111/j.1365-2672.2006.03084.x
Hsu, C.K., Liao, J.W., Chung, Y.C., Hsieh, C.P. and Chan, Y.C. (2004) Xylooligosaccharides and Fructooligosaccharides Affect the Intestinal Microbiota and Precancerous Colonic Lesion Development in Rats. The Journal of Nutrition, 134, 1523-1528. https://doi.org/10.1093/jn/134.6.1523
Rossi, M., Corradini, C., Amaretti, A., Nicolini, M., Pompei, A., Zanoni, S. and Matteuzzi, D. (2005) Fermentation Offructooligosaccharides and Inulin by Bifidobacteria: A Comparative Study of Pure and Fecal Cultures. Applied and Environmental Microbiology, 71, 6150-6158. https://doi.org/10.1128/AEM.71.10.6150-6158.2005
Santànna, M.S.L. and Ferreira, C.L.L.F. (2014) Can Intestinal Constipation Be Modulated by Prebiotics, Probiotics and Symbiotics? Food & Nutrition Sciences, 5, 1106-1113. https://doi.org/10.4236/fns.2014.512120
Lu, Z.X., Gibson, P.R., Muir, J.G., Fielding, M. and O’Dea, K. (2000) Arabinoxylan Fiber from a By-Product of Wheat Flour Processing Behaves Physiologically like a Soluble, Fermentable Fiber in the Large Bowel of Rats. Journal of Nutrition, 130, 1984-1990. https://doi.org/10.1093/jn/130.8.1984
Paturi, G., Butts, C.A., Stoklosinski, H. and Ansell, J. (2012) Effects of Early Dietary Intervention with a Fermentable Fibre on Colonic Microbiota Activity and Mucin Gene Expression in Newly Weaned Rats. Journal of Functional Foods, 4, 520-530. https://doi.org/10.1016/j.jff.2012.02.013
Scheppach, W., Sommer, H., Kirchner, T., Paganelli, G.M., Bartram, P., Christl, S., et al. (1992) Effect of Butyrate Enemas on the Colonic Mucosa in Distal Ulcerative Colitis. Gastroenterology, 103, 51-56. https://doi.org/10.1016/0016-5085(92)91094-K
Kleessen, B., Hartmann, L. and Blaut, M. (2014) Oligofructose and Long-Chain Inulin: Influence on the Gut Microbial Ecology of Rats Associated with a Human Faecal Flora. British Journal of Nutrition, 86, 291-300. https://doi.org/10.1038/nature12820
David, L.A., et al. (2014) Diet Rapidly and Reproducibly Alters the Human Gut Microbiome. Nature, 505, 559-563.