Cyanidine-3-O-Galactoside Enriched <i>Aronia melanocarpa</i> Extract Inhibits Adipogenesis and Lipogenesis via Down-Regulation of Adipogenic Transcription Factors and Their Target Genes in 3T3-L1 Cells — Oak Academic Publishing
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Cyanidine-3-O-Galactoside Enriched <i>Aronia melanocarpa</i> Extract Inhibits Adipogenesis and Lipogenesis via Down-Regulation of Adipogenic Transcription Factors and Their Target Genes in 3T3-L1 Cells
Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Chuncheon, Republic of Korea
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Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Chuncheon, Republic of Korea
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R&D Center, Wellfine Co., Ltd., Chuncheon, Republic of Korea
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R&D Center, Wellfine Co., Ltd., Chuncheon, Republic of Korea
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Department of Food Science & Nutrition, Dongseo University, Busan, Republic of Korea
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Department of Food Science & Nutrition, Dongseo University, Busan, Republic of Korea
1 Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Chuncheon, Republic of Korea
2 Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Chuncheon, Republic of Korea
3 R&D Center, Wellfine Co., Ltd., Chuncheon, Republic of Korea
4 R&D Center, Wellfine Co., Ltd., Chuncheon, Republic of Korea
5 Department of Food Science & Nutrition, Dongseo University, Busan, Republic of Korea
6 Department of Food Science & Nutrition, Dongseo University, Busan, Republic of Korea
Aronia melamocarpa (AM) is a rich source of anthocyanins, which are known to help prevent obesity. The cyanidine-3-O-galactoside enriched AM extract (AM-Ex) containing more cyanidine-3-O-galactoside than conventional AM extract was recently developed. The objective of this study was to examine the effect of AM-Ex on adipogenesis and its action mechanisms in vitro using 3T3-L1 adipocytes. To examine the anti-obesity effect of AM-Ex, 3T3-L1 cells were induced adipocyte differentiation and incubated with various concentration of AM-Ex. Lipid accumulation, cellular triglyceride content, mRNA expression of transcription factors and adipogenic genes were analyzed. Treatment with 100 - 400 μg/mL of AM-Ex resulted in a dose-dependent decrease in adipocyte differentiation and triglyceride accumulation. mRNA expression of adipogenic transcription factors, such as peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein α , sterol regulatory element-binding protein 1 were decreased. The level of gene expression of adipogenesis and lipogenesis-related genes, such as adipocyte protein 2, lipoprotein lipase, acetyl-CoA carboxylase, ATP-citrate lyase and fatty acid synthase were decreased. These results suggest that AM-Ex alleviated risk factors related to obesity by modulating multiple pathways associated with adipogenesis.
KeywordsObesityAdipocyteAdipogenesisLipogenesisTranscription FactorAdipocyte Protein 2
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