A Comparative Study on the Effect of <i>Massularia acuminata</i> Fractions against Bacterial and Fungal Isolates from the Oral Cavity
- 1 Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria
- 2 Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria
- 3 Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria
- 4 Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria
Abstract
The research aimed at carrying out a comparative study on the effect of Massularia acuminata fractions against bacterial and fungi isolates from the oral cavity. The Antimicrobial assay was carried on the following test organism; Staphylococcus aureus , Klebsiella pnuemoniae , Bacillus subtilis and Escherichia coli and Enterobacter aerogenes . From antifungal result, it was observed that the highest inhibition was observed in the ethanolic extract of Massularia acuminata on Rhyzopus stolonifer , Candida albican and Fusarium solani . The ethanolic and methanolic extracts were also observed from the results to have an intermediate sensitivity to Aspergillus favus , Candida tropicalis , Can dida albican , Rhyzopus stolonifer , Fusarium solani , while it was observed to be resistant to Fusarium solani , Rhyzopus stolonifer , Candida tropicalis , Aspergillus favus for all the partitions of the extracts. From the antifungal effect of the M . acuminata fractions, it could be inferred that the ethanolic and methanolic extracts were quite effective in the inhibition of the fungi infections and the methanolic extract proved very infective on Candida albican , Rhyzopus stolonifer and Fusarium solani . From the result of the antibacterial effect of M . acuminata fractions, the highest inhibition was observed by the methanolic extract of Massularia acuminata against all the Gram negative bacteria and Staphylococcus aureus . Also the aqueous extract also had a less sensitivity against all the test microbes, as all proved resistant. It could be inferred that the fractions of the extracts were unable to inhibit the growth of the strain of gram positive bacteria and probably, the potency could be increased, if the concentrations of the methanolic, ethanolic extracts of Massularia acuminata and the standard drug, tetracycline are increased. Results obtained showed that ethanol was a better and more powerful solvent than water. Whereas, the methanolic extract and the standard drug were quite effective in the inhibition of some gram negative bacteria, Klebsiella pnuemoniae and Enterobacter aerogenes . From the results obtained, it can be deduced that the ethanolic and methanolic extracts of Massularia acuminta possess antifungal and antibacterial potentials, compared with the aqueous extract and its potency as an inhibitor of bacterial and fungi organism found in the oral cavity.
- Alderberth, I. and Wold, A.E. (2009) Establishment of the Gut Microbiota in Western Infants. Acta Paediatrica, 98, 229-238. https://doi.org/10.1111/j.1651-2227.2008.01060.x
- Aagaard, K., Ma, J., Anthony, K.M., Ganu, R. and Petrosino, J. (2014) The Placenta Harbors a Unique Microbiome. Science Translational Medicine, 6, 273ra65. https://doi.org/10.1126/scitranslmed.3008599
- Maffei, V.J., Kim, S., Blanchard, E., Luo, M., Jazwinski, S.M. and Taylor, C.M. (2017) Biological Aging and the Human Gut Microbiota. The Journals of Gerontology Series A Biological Sciences and Medical Sciences, 2, 147-1482. https://doi.org/10.1093/gerona/glx042
- Socransky, S.S. and Haffajee, A.D. (2005) Periodontal Microbial Ecology. Periodontology, 38, 135-187. https://doi.org/10.1111/j.1600-0757.2005.00107.x
- Segata, N., Waldron, L., Ballarini, A., Narasimhan, V., Jousson, O. and Huttenhower, C. (2012) Metagenomic Microbial Community Profiling Using Unique Clade-Specific Marker Genes. Nature Methods, 9, 811-814. https://doi.org/10.1038/nmeth.2066
- Trojanowska, D., Zwolinska-Wcislo, M., Tokarczyk, M., Kosowski, K., Mach, T. and Budak, A. (2010) The Role of Candida in Inflammatory Bowel Disease. Estimation of Transmission of C. albicans Fungi in Gastrointestinal Tract Based on Genetic Affinity between Strains. Medical Science Monitor, 16, 451-457.
- Arumugam, M., Raes, J., Pelletier, E., Pastier, D.L., et al. (2011) Enterotypes of the Human Gut Microbiome. Nature, 473, 174-180. https://doi.org/10.1038/nature09944
- Qin, J., Li, Y., Cai, Z., Li, S., Zhu, J., Zhang, W., Guan, Y. and Shen, D. (2012) A Metagenome-Wide Association Study of Gut Microbiota in Type 2 Diabetes. Nature, 490, 55-60. https://doi.org/10.1038/nature11450
- Ferlay, J., et al. (2015) Cancer Incidence and Mortality Worldwide: Sources, Methods and Major Patterns in GLOBOCAN 2012. International Journal of Cancer, 136, E359-E386. https://doi.org/10.1002/ijc.29210
- Arthur, J.C. and Jobin, C. (2011) The Struggle within: Microbial Influences on Colorectal Cancer. Inflammatory Bowel Diseases, 17, 396-409. https://doi.org/10.1002/ibd.21354
- Andrews, J.M. (2009) BSAC Standardized Disc Susceptibility Testing Method (Version 8). Journal of Antimicrobial Chemotherapy, 64, 454-489. https://doi.org/10.1093/jac/dkp244
- Willey, J.M., Sherwood, L.M. and Woolverton, C.J. (2008) Prescott, Harley and Klein’s Microbiology. 7th Edition, McGraw Hill, New York.