An Antibacterial Wound Dressing Based on GS-SF Composite Scaffold
- 1 Department of Medical Information, Chongqing Medical University, Chongqing, China
- 2 Experimental Education Center, Chongqing Medical University, Chongqing, China
- 3 Department of Medical Information, Chongqing Medical University, Chongqing, China
Abstract
Although the treatment of burn wounds has made great progress, the incidence of wound infection is still the main cause of high mortality. In this study, a silk fibroin (SF) scaffold wound dressing incorporated with Gentamicin Sulfate (GS) was developed for the treatment of burn infected wounds, in which GS was used as anti-bacterial agent. GS was mixed with silk fibroin solution and then processed into GS-SF composite scaffold via electro-spinning. The results showed the scaffold exhibited uniform polyporous morphology with 80% porosity. Induced by methanol, the scaffold presented much improved mechanical properties and stability to protease XIV . More important, the scaffold presented significant growth inhibition on both Gram-positive ( Staphylococcus aureus ) and Gram-negative ( Pseudomonas aeruginosa and Escherichia coli ) bacteria.
- Church, D., Elsayed, S. and Reid, O. (2006) Burn Wound Infections. Clinical Microbiology Reviews, 19, 403-434. https://doi.org/10.1128/CMR.19.2.403-434.2006
- Salerian, A.J. (2020) Burn Wound Infections and Pseudomonas aeruginosa. Burns, 46, 257-258. https://doi.org/10.1016/j.burns.2019.07.008
- Rebecca, R., Adrien, P. and Melissa, B. (2019) Burn Wound Classification Model Using Spatial Frequency-Domain Imaging and Machine Learning. Journal of Biomedical Optics, 24, 1-9. https://doi.org/10.1117/1.JBO.24.5.056007
- Banani, K., Rangam, R., Subhas, C. and Wang, X.-G. (2013) Silk Fibroin Biomaterials for Tissue Regenerations. Advanced Drug Delivery Reviews, 65, 457-470. https://doi.org/10.1016/j.addr.2012.09.043
- Joydip, K., Laura, A., Poole, W., Penny, M. and Subhas, K. (2012) Silk Fibroin/Poly(Vinyl Alcohol) Photo-Crosslinked Hydrogels for Delivery of Macromolecular Drugs. Acta Biomaterialia, 8, 1720-1729. https://doi.org/10.1016/j.actbio.2012.01.004
- Vepari, C. and Kaplan, D.L. (2007) Silk as a Biomaterial. Progress in Polymer Science, 32, 991-1007. https://doi.org/10.1016/j.progpolymsci.2007.05.013
- Wenk, E., et al. (2008) Silk Fibroin Spheres as a Platform for Controlled Drug Delivery. Journal of Controlled Release, 132, 26-34. https://doi.org/10.1016/j.jconrel.2008.08.005
- Nicholas, G., Annie, B., Bernardo, P. and Kaplan, D.L. (2011) Lyophilized Silk Fibroin Hydrogels for the Sustained Local Delivery of Therapeutic Monoclonal Antibodies. Biomaterials, 32, 2642-2650. https://doi.org/10.1016/j.biomaterials.2010.12.023
- Wang, Y.-Z., Kim, H.-J., Gordana, N. and Kaplan, D.L. (2006) Stem Cell-Based Tissue Engineering with Silk Biomaterials. Biomaterials, 27, 6064-6082. https://doi.org/10.1016/j.biomaterials.2006.07.008
- Joydip, K., Moumita, D., Sarani, G. and Kundu, S.C. (2008) Mulberry Non-Engineered Silk Gland Protein Vis-a-Vis Silk Cocoon Protein Engineered by Silkworms as Biomaterial Matrices. Journal of Materials Science: Materials in Medicine, 19, 2679-2689. https://doi.org/10.1007/s10856-008-3398-1
- She, H.-D., Xiao, X.-F. and Liu, R.-F. (2007) Preparation and Characterization of Polycaprolactone-Chitosan Composites for Tissue Engineering Applications. Journal of Material Science, 42, 8113-8119. https://doi.org/10.1007/s10853-007-1706-7
- Panya, W., Hiroki, U., Yasuhiko, T. and Ratana, R. (2010) In Vitro and in Vivo Release of Basic Fibroblast Growth Factor Using a Silk Fibroin Scaffold as Delivery Carrier. Journal of Biomaterials Science, Polymer Edition, 21, 1403-1419. https://doi.org/10.1163/092050609X12517858243706