Inhibition of H2O2-induced DNA damage in single cell gel electrophoresis assay (comet assay) by castasterone isolated from leaves of centella asiatica — Oak Academic Publishing
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Inhibition of H2O2-induced DNA damage in single cell gel electrophoresis assay (comet assay) by castasterone isolated from leaves of centella asiatica
Brassinosteroids (BRs) are a large group of polyhydroxy steroids, which regulate numerous aspects of plant growth and development, including stem elongation, leaf bending, tracheary element differentiation, stress protection and photomorphogenesis. Recent studies indicate antigenotoxic and anticancerous activities of these compounds. The role of natural BRs in H2O2 (hydrogen peroxide) -induced DNA damage in human lymphocytes is still unknown. The present study reports the presence of Castasterone from leaves of Centella asiatica, an important medicinal herb commonly used as a memory enhancer and immunomodulator. CA50 fraction isolated from Centella asiatica was characterized as Castasterone by electrospray ionization mass spectral data with standard Castasterone. An attempt has been made to study antigenotoxic activity of the isolated Castasterone against H2O2 -induced DNA damage in human blood lymphocytes using Single cell gel electrophoresis assay (Comet Assay). Castasterone at 10–9 M concentration proved to be effective in diminishing the DNA damage by 89.42 %.
Halliwell, B. and Gutteridge, J.M. (2007) Free Radicals in Biology and Medicine. 4th Edition, Oxford University Press, USA.
Vahala, J., Keinanen, M., Schutzendubel, A., Polle, A. and Kangasjarvi, J. (2003) Differential effects of elevated ozone on two hybrid aspen genotypes predisposed to chronic ozone fumigation. Role of ethylene and salicylic acid. Plant Physiology, 132(1), 196-205.
Kovtun, Y., Chiu, W.L., Tena, G. and Sheen, J. (2000) Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proceedings of National Academy of Sciences, USA, 97(6), 2940-2945.
Cao, S., Xu, Q., Cao, Y., Qian, K., An, K., Zhu, Y., Binzeng, H., Zhao, H. and Kuai, B. (2005) Loss-of- function mutation in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Physiologia Plantarum, 123(1), 57-66.
Sasse, J.M. (2003) Physiological actions of brassinosteroids: An update. Journal of Plant Growth Regulation, 22 (4), 276-288.
Wachsman, M.B., Ramirez, J.A., Galagovsky, L.R. and Coto, C.E. (2002) Antiviral activity of brassinosteroids derivatives against measles virus in cell cultures. Antiviral Chemistry and Chemotherapy, 13(1), 61-66.
Wachsman, M.B., Castilla, V., Talarico, L.B., Ramirez, J.A., Galagovsky, L.R. and Coto, C.E. (2004) Antiher-patic mode of action of (22S, 23S)-3beta-Bromo-5alpha, 22, 23-trihydroxystigmastan-6-one in vitro. International Journal of Antimicrobial Agents, 23(5), 524-526.
Romanutti, C., Castilla, V., Coto, C.E. and Wachsman, M.B. (2007) Antiviral effect of a synthetic brassinosteroid on the replication of vesicular stomatitis virus in Vero cells. International Journal of Antimicrobial Agents, 29 (3), 311-316.
Franek, F., Eckschlager, T. and Kohout, L. (2003) 24-epibrassinolide at subnanomolar concentrations modulates growth and production characteristics of a mouse hybridoma. Collection of Czechoslovak Chemical Communications, 68(11), 2190-2200.
Malıkova, J., Swaczynova, J., Kolar, Z. and Strnad, M. (2008) Anticancer and antiproliferative activity of natural brassinosteroids. Phytochemistry, 69(2), 418-426.
Swaczynova, J., Malikova, J., Hoffmannova, L., Kohout, L. and Strnad, M. (2006) Anticancer properties of brassinosteroids. Planta Medica, 72.
Wang, X.S., Zheng, Y., Zuo, J. and Fang, J. (2005) Structural features of an immunoactive acidic arabinogalactan from Centalla asiatica. Carbohydrate Polymers, 59(3), 281-288.
Zainol, M.K., Abd-Hamid, A., Yusof, S. and Muse, R. (2003) Antioxidant activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L) Urban. Food Chemistry, 81(4), 575- 581.
Gnanapragasam, A., Ebenezar, K.K., Satish, V., govindaraju, P. and Devaki, T. (2004) Protective effects of Centella asiatica on antioxidant tissue defence system against adriamycin induced cardiomyopathy in rats. Life Sciences, 76(5), 585-597.
Bunpo, P., Kataoka, K. and Arimochi, H. (2004) Inhibitory effect of Centella asiatica on azoxymethane-induced aberrant crypt focus formation and carcinogenesis in the intestines of F344 rats. Food and Chemical Toxicology, 42(12), 1987-1997.
Cheng, C.L., Guo, J.S., Luk, J. and Koo, M.W.L. (2004) The healing effets of Centella asiatica extrats and asiaticoside on acetic acid induced gastric ulcers in rats. Life Sciences, 74(18), 2237-2249.
Takatsuto, S., Yazawa, N., Ikekawa, N., Takematsu, T., Takeuchi, Y. and Koguchi, M. (1983) Structural-activity relationship of Brassinosteroids. Phytochemistry, 22(11), 2437-2441.
Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1), 184-191.
Anderson, D., Dobrzynska, M.M., Basaran, N., Basaran, A. and Yu, T.W. (1998) Flavonoids modulate Comet as- say response to food mutagens in human lymphocytes and sperm. Mutation Research, 402(1-2), 269-277.
Boyum, A. (1968) Separation of leucocytes from blood and bone marrow. Scandinavian Journal of Clinical and Laboratory Investigation, 21(Suppl 97), 78-89
Mayers, L.S. and Grossen, N.E. (1974) Analysis of independent group designs. In. Meyers, L.S. and Grossen, N.E. Eds., Behavioural Research, Theory, Procedure and Design, W.H. Freeman, San Francisco.
Fujioka, S., Noguchi, T., Yokota, T., Takatsuto, S. and Yoshida, S. (1998) Brassinosteroids in Arabidopsis thaliana. Phytochemistry, 48(4), 595-599
Schmidt, J., Porzel, A. and Adam, G. (1998) Brassinosteroids and a pregnane glucoside from Daucus carota. Phytochemical Analysis, 9(1), 14-20.
Yokota, T., Higuchi, K., Takahashi, N., Kamuro, Y., Watanabe, T. and Takatsuto, S. (1998) Identification of brassinosteroids with epimerized substituents and/or the 23-Oxo group in pollen and anthers of Japanese Cedar. Bioscience Biotechnology and Bichemistry, 62(3), 526- 531
Onatskiy, N.M., Marchenko, A.I. and Mikhina, L.V. (1997) Technical Report: Evaluation of mutagenic active- ity of epibrassinolide (active ingredient of Epin) in Ames test, chromosome aberrations and in micronuclear tests. Scientific Research Centre of Toxicologie and Hygienic Regulation of Biopreparations of Russia, Serpukhov.
Sondhi, N.P., Bhardwaj, R., Kaur, S., Kumar, N. and Singh, B. (2008) Isolation of 24-epibrassinolide from leaves of Aegle marmelos and evaluation of its anti-genotoxicity employing Allium cepa chromosomal aberration assay. Plant Growth Regulation, 54(3), 217-224.
Merzenich, U.G., Zeitler, H., Vetter, H. and Kraft, K. (2008) Synergy research: Vitamins and secondary plant components in the maintenance of the redox homeostasis and cell signaling. Phytomedicine, 16(1), 2-16.
Wu, Y.D. and Lou, Y.J. (2007) Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostrate cancer PC-3 cells. Die Pharmazie, 62(5), 392-395.
Slavikova, B., Kohout, L., Budesinsky, M., Swaczynova, J. and Kasal, A. (2008) Brassinosteroids: Synthesis and activity of some fluoro analogues. Journal of Medicinal Chemistry, 51(13), 3979-3984
Mooradian, A.D. (1993) Antioxidant properties of steroids. Journal of steroid Biochemistry and Molecular Biology, 45(6), 509-511.
Krishna, P. (2003) Brassinosteroid-mediated stress responses. Journal of Plant Growth Regulation, 22(4), 289- 297.