Effect of mango seed kernel extract on the adipogenesis in 3T3-L1 adipocytes and in rats fed a high fat diet
- 1 Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan
- 2 Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan
- 3 Nitta Gelatin Inc., Yao, Japan
- 4 Nitta Gelatin Inc., Yao, Japan
- 5 Department of Biochemistry, Kinki University Medical School, Osaka-Sayama, Japan
- 6 Department of Biochemistry, Kinki University Medical School, Osaka-Sayama, Japan
- 7 Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan
Abstract
Mangoes ( Mangifera indica L.) are one of the most important tropical foods. The seed is one of the main by-products of mango processing. Therefore, it is important to find an economically viable use for this waste (e.g., as a food additive or supplement with high nutraceutical value). We investigated the anti-obesity effects of mango seed kernel extract with hot water (MSKE-W) in 3T3-L1 adipocytes and in a high fat diet (HFD)-induced obesity rat model. MSKE-W caused a significant decrease in the activity of glycerol 2-phosphate dehydrogenase in 3T3-L1 adipocytes without eliciting cell cytotoxicity and inhibited cellular lipid accumulation through down-regulation of transcription factors such as PPAR γ and C/EBP α . In the animal model, rats fed an HFD containing 1% MSKE-W gained less weight than rats fed an HFD alone. The visceral fat mass in rats fed an HFD containing 1% MSKE-W tended to be lower than that in rats fed an HFD alone. Furthermore, histological examination of rat livers from an HFD showed steatohepatitis. However, rats on an HFD containning 1% MSKE-W showed no histopathological changes in liver tissue. Our results indicate that MSKE-W influences anti-obesity effects, both in vitro and in vivo , and suggest that MSKE-W provides a novel preventive potential against obesity.
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