Gonadotropin and Testosterone hormone’s serum levels and partial deletions in the AZFc region in Iranian oligozoospermia infertile males — Oak Academic Publishing
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Gonadotropin and Testosterone hormone’s serum levels and partial deletions in the AZFc region in Iranian oligozoospermia infertile males
To investigate the relation of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and Testosterone serum levels with partial deletions in the AZFc region in Iranian oligozoospermia males. Material and methods: thirty infertile oligozoospermia and 52 Iranian fertile men included. The hormonal assays were measured by the Radioimmunoassay (RIA). Multiplex polymerase chain reaction (M-PCR) using eight sequence-tagged site (STS) markers were measured on the Yq11 chromosome. Results: The mean of FSH and LH levels in all oligozoospermia males were higher than fertile men (p < 0.001) and testosterone was lower significantly (p < 0.001). Five patients showed partial deletions in AZFc region (four had gr/gr and one had b2/b3 deletions). Six fertile men showed partial deletions (five gr/gr and one b2/b3) with higher level of FSH, LH in their group (p < 0.05). Conclusion: According to high incidence of partial deletions in the AZFc region among Iranian oligozoospermia males, hormonal assay and molecular screening should be advised before considering for ART treatments.
Mittal, R.D., Singh, G., Srivastava, A., Pradhan, M., Kesari, A., Makker, A. and Mittal, B.Y. (2004) Chromosome micro-deletions in idiopathic infertility from Northern India. Annales de Génétique, 47, 331-337. doi:10.1016/j.anngen.2004.05.003
Lynch, M., Cram, D.S., Reilly, A., O’Bryan, M.K. and Baker, H.W.G. (2005) The Y chromosome gr/gr subdeletion is associated with male infertility. Molecular Human Reproduction, 11, 507-512. doi:10.1093/molehr/gah191
Madhukar, D. and Rajender, S. (2009) Hormonal treatment of male infertility: Promises and Pitfalls. Journal of Andrology, 30, 95-112. doi:10.2164/jandrol.108.005694
Raicu, F., Popa, L., Apostol, P., Cimponeriu, D., Danl, L., Ilinca, E., et al. (2003) Screening for microdeletions in human Y chromosome AZF candidate genes and male infertility. Journal of Cellular and Molecular Medicine., 7, 43-48. doi:10.1111/j.1582-4934.2003.tb00201.x
Tiepolo, L. and Zuffardi, O. (1976) Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long arm. Human Genetics, 34, 119-124. doi:10.1007/BF00278879
Reijo, R., Lee, T.Y., Salo, P., Alagappan, R., Brown, L.G., Rosenberg, M., et al. (1995) Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Nature Genetics, 10, 383-393. doi:10.1038/ng0895-383
Vogt, P.H., Edelmann, A., Kirsch, S., Henegariu, O., Hirschmann, P., Kiesewetter, F., et al. (1996) Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yq11. Human Molecular Genetics, 5, 933-943. doi:10.1093/hmg/5.7.933
Kent-First, M., Muallem, A., Shultz , J., Pryor, J., Roberts, K., Nolten, W., et al. (1999) Defining regions of the Y-chromosome responsible for male infertility and identification of a fourth AZF region (AZFd) by Y-chromosome microdeletion detection. Molecular Reproduction and Development, 53, 27-41. doi:10.1002/(SICI)1098-2795(199905)53:1 3.0.CO;2-W
Ferlin, A., Tessari, A., Ganz, F., Marchina, E., Barlati, S., Garolla, A., et al. (2005) Association of partial AZFc region deletions with spermatogenesis impairment and male infertility. Journal of Medical Genetics, 42, 209-213. doi:10.1136/jmg.2004.025833
Repping, S., Skaletsky, H., Brown, L., Van Daalen, S.K., Korver, C.M., Pyntikova, T., et al. (2003) Polymorphism for a 1.6-Mb deletion of the human Y chromosome persists through balance between recurrent mutation and haploid selection. Nature Genetics, 35, 247-251. doi:10.1038/ng1250
Fernandes, S., Huellen, K., Goncalves, J., Dukal, H., Zeisle, J., Souse, M., et al. (2002) High frequency of DAZ1/DAZ2 gene deletions in patient with severe oligozoospermia. Molecular Human Reproduction, 8, 286-298. doi:10.1093/molehr/8.3.286
Repping, S., Saskia, K.M., Van Daalen, S.K., Korver , C. M., Brown, L.G., Marszalek, J.D., et al. (2004) A family of human Y chromosomes has dispersed throughout northern Eurasia despite a 1.8-Mb deletion in the azoospermia factor c region. Genomics, 83, 1046-1052. doi:10.1016/j.ygeno.2003.12.018
Simoni, M., Bakker, E. and Krausz, C. (2004) EAA/EMQN best practice guidelines for molecular diagnosis of Y-chromosomal microdeletions. State of the ART 2004. International Journal of Andrology, 27, 240-249. doi:10.1111/j.1365-2605.2004.00495.x
Mirfakhraie, R., Mirzajani, F., Mahdi Kalantar, S., Montazeri, M., Salsabili, N., Pourmand, G.R. and Houshmand, M. (2010) High prevalence of AZFb microdeletion in Iranian patients with idiopathic non-obstructive azoospermia. Indian Journal of Medical Research, 132, 265-270.
Babu, S., Sadhnanl, M.D., Swama, M., Padmavathi, P. and Reddy, P.P. (2004) Evaluation of FSH, LH and testosterone levels in different subgroups of infertile males. Indian Journal of Clinical Biochemistry, 19, 45-49. doi:10.1007/BF02872388
Ferlin, A., Arredi, B.A., E Speltra., et al. (2007) Molecular and clinical characterization of Y chromosome microdeletions in infertile men: A 10-year experience in Italy. The Journal of Clinical Endocrinology & Metabolism, 97, 762-770.
Nowroozi, M.R. and Radkhah, K. (2008) Serum inhibin B concentration as a prognostic factor for prediction of sperm retrieval in testis biopsy of patients with azoospermia. Archives of Iranian Medicine, 11, 54-56.
Kumar, R., Dada, R. and Gupta, N.P. (2006) Serum FSH levels and testicular histology in infertile men with non obstructive azoospermia and Y chromosome microdeletions. Indian Journal of Urology, 22, 332-336. doi:10.4103/0970-1591.29119
Claudia, C.M.B., Zuccherato, L.W., Bastos-Rodrigues, L., Santos, F.R. and Pena, S.D.J. (2006) No association found between gr/gr deletions and infertility in Brazilian males. Molecular Human Reproduction, 12, 269-273. doi:10.1093/molehr/gal029
Oates, R.D., Silber, S., Brown, L.G. and Page, D.C. (2002) Clinical characterization of 42 oligospermic or azoospermic men with microdeletion of the AZFc region of the Y chromosome, and of 18 children conceived via ICSI. Human Reproduction, 17, 2813-2824. doi:10.1093/humrep/17.11.2813
Zhang, F., Li, Z., Wen, B., Jiang, J., Shao, M., Zhao, Y., et al. (2006) A frequent partial AZFc deletion does not render an increased risk of spermatogenic impairment in East Asians. Annals of Human Genetics, 70, 304-313. doi:10.1111/j.1529-8817.2005.00231.x
Olive, A., Spira, A. and Multigner, L. (2001) Contribution of environmental factors to the risk of male infertility. Human Reproduction, 16, 1768-1776. doi:10.1093/humrep/16.8.1768
Carrara, R.C.V., Yamasaki, R., Mazucatto, L.F., Veludo, M.A.L., et al. (2004) Somatic and grem cell cytogenetic studies and AZF microdeletion screening in infertile men. Genetics and Molecular Biology, 27, 477-482. doi:10.1590/S1415-47572004000400002
Hellani, A., Al-Huellen, S., Iqbal, M. and Coskun, S. (2006) Y chromosome microdeletion in infertile men with idiopathic olig- or azoospermia. Journal of Experimental & Clinical Assisted Reproduction, 3, 1. doi:10.1186/1743-1050-3-1