Antiprotozoal Activity of a <i>Thymus vulgaris</i> Methanol Extract and Its Fractions — Oak Academic Publishing
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Antiprotozoal Activity of a <i>Thymus vulgaris</i> Methanol Extract and Its Fractions
Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
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Laboratory of Biochemistry and Cell Physiology, Division of Cellular and Molecular Biology, Center for Biomedical Research of the Northeast, Mexican Social Security Institute, Monterrey, Mexico
,
Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
,
Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
,
Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
,
Laboratory of Biochemistry and Cell Physiology, Division of Cellular and Molecular Biology, Center for Biomedical Research of the Northeast, Mexican Social Security Institute, Monterrey, Mexico
,
Mexican Social Security Institute, Nuevo Leon Delegation, Monterrey, Mexico
,
Department of Basic Sciences, Division of Health Sciences, University of Monterrey, San Pedro Garza Garcia, Mexico
1 Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
2 Laboratory of Biochemistry and Cell Physiology, Division of Cellular and Molecular Biology, Center for Biomedical Research of the Northeast, Mexican Social Security Institute, Monterrey, Mexico
3 Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
4 Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
5 Department of Chemistry, Phytochemistry and Analytical Chemistry Lab, Faculty of Biological Sciences, University Autonomous of Nuevo Leon, San Nicolas de los Garza, Mexico
6 Laboratory of Biochemistry and Cell Physiology, Division of Cellular and Molecular Biology, Center for Biomedical Research of the Northeast, Mexican Social Security Institute, Monterrey, Mexico
7 Mexican Social Security Institute, Nuevo Leon Delegation, Monterrey, Mexico
8 Department of Basic Sciences, Division of Health Sciences, University of Monterrey, San Pedro Garza Garcia, Mexico
Introduction : Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective : To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods : Conventional cultures of parasites were measured on the third day during the logarithmic growth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts. Next, the extract was fractionated by polarity-based partitioning. Then, the purity of each fraction was determined by thin layer chromatography (TLC). The percentage of growth inhibition was calculated with respect to untreated controls. The 50% inhibitory concentration (IC 50 ) of each extract was calculated by PROBIT analysis. Results : We found that a methanol extract of the aerial parts of Thymus vulgaris , at 300 μg/mL, inhibited the in vitro growth of G. lamblia and T. vaginalis , while E. histolytica growth was poorly inhibited. The methanol extract was further separated into mixtures of ursolic, oleanolic, and betulinic acids. The IC 50 values of ursolic acid against G. lamblia and T. vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions : The methanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites.
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