Associations between Placental Insulin-Like Growth Factor-1 Gene Expression, DNA Methylation and Intrauterine Growth Restriction — Oak Academic Publishing
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Associations between Placental Insulin-Like Growth Factor-1 Gene Expression, DNA Methylation and Intrauterine Growth Restriction
Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
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Department of Pediatrics, University of Utah, Salt Lake City, Utah, USA
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Haikou City Maternal and Child Care Hospital Clinical Laboratory, Haikou, China
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Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
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Tropical Biological Technology Research Institute, Chinese Academy of Tropical Agriculture Science, Haikou, China
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Tropical Biological Technology Research Institute, Chinese Academy of Tropical Agriculture Science, Haikou, China
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Prenatal Diagnosis Center, Hainan Medical Center for Women and Children, Haikou, China
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Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
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Hainan Medical College, Haikou, China
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Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
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Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
1 Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
2 Department of Pediatrics, University of Utah, Salt Lake City, Utah, USA
3 Haikou City Maternal and Child Care Hospital Clinical Laboratory, Haikou, China
4 Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
5 Tropical Biological Technology Research Institute, Chinese Academy of Tropical Agriculture Science, Haikou, China
6 Tropical Biological Technology Research Institute, Chinese Academy of Tropical Agriculture Science, Haikou, China
7 Prenatal Diagnosis Center, Hainan Medical Center for Women and Children, Haikou, China
8 Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
9 Hainan Medical College, Haikou, China
10 Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
11 Hainan Provincial People’s Hospital Central Laboratory, Key Laboratory of Cellular and Molecular Genetic Transformation of Hainan Province Medicine, Haikou, China
Intrauterine growth restriction (IUGR) is a common fetal development disorder which has great impact on neonatal health. Insulin-like growth factor-1 ( IGF 1) has an important role in regulating fetal growth. Whether IGF 1 DNA methylation was associated with IUGR has not been studied. Placenta samples from IUGR (n = 27) and normal delivery (n = 29) were collected whereas basic information of mothers and infants were also collected. RT-PCR was performed to examine IGF 1 transcriptions and bisulfite sequencing PCR was used for DNA methylation analysis. Gene expression analysis found IUGR had significantly lower IGF 1 transcription compared to control group (IUGR: 0.330 ± 0.351; control group: 1.001 ± 0.800, t = 3.995, P < 0.001). CpG sites at the promoter region of IGF 1 were all highly methylated and there is no difference on DNA methylation rate between IUGR and control group (IUGR: 75%; control group: 81%; P = 0.09). Interestingly, in both IUGR and control groups, male fetus had significantly higher methylation rate than female fetus (IUGR: male: 87%; female: 74%, P = 0.016; control: male: 82%; female: 69%, P = 0.012). There was no correlation between IGF 1gene expression and DNA methylation rate (r = 0.095, P = 0.063). Intrauterine fetal growth restriction placenta had significantly lower IGF 1gene expression; however, IGF 1 DNA methylation level was similar. A potential fetus gender difference was also found in IGF 1 DNA methylation rate.
KeywordsPlacentalInsulin-Like Growth Factor-1IUGR
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