Purpose: To establish whether Curaderm, a topical pharmacotherapy for skin cancer, irritates or sensitizes normal skin. Methods: The dermal irritation and skin sensitization toxicity of Curaderm were investigated in rabbits and guinea pigs in compliance with the Organization for Economic Cooperation and Development guideline. To assess dermal irritation, rabbits were dermally exposed to Curaderm for varying periods of time. To assess hypersensitivity, the guinea-pig maximisation test was applied. Results: Curaderm was only negligibly irritating using the criteria of erythema and oedema. Curaderm did not produce any sensitization toxicity of the skin. Conclusion: These studies confirm the non-toxic observations on normal skin experienced in the clinical setting when treating skin cancer and reinforce the specificity of Curaderm towards cancer cells.
Cham, B.E. and Wilson, L. (1987) HPLC of Glycoalkaloids from Solanum sodomaeum. Planta Medica, 1, 59-62. https://doi.org/10.1055/s-2006-962621
Cham, B.E. (1988) Monograph BEC. Drugs of the Future, 13, 714-716. https://doi.org/10.1358/dof.1988.013.08.63026
Daunter, B. and Cham, B.E. (1990) Solasodine Glycosides. In Vitro Preferential Cytotoxicity for Human Cancer Cells. Cancer Letters, 55, 209-220. https://doi.org/10.1016/0304-3835(90)90121-D
Cham, B.E. and Daunter, B. (1990) Solasodine Glycosides. Selective Cytotoxicity for Cancer Cells and Inhibition of Cytotoxicity by Rhamnose in Mice with Sarcoma 180. Cancer Letters, 55, 221-225. https://doi.org/10.1016/0304-3835(90)90122-E
Cham, B.E. (2017) Solasodine, Solamargine and Mixtures of Solasodine Rhamnosides: Pathway to Expansive Clinical Anticancer Therapies. International Journal of Clinical Medicine, 8, 692-713. https://doi.org/10.4236/ijcm.2017.812064
Cham, B.E. and Daunter, B. (1990) Topical Treatment of Pre-Malignant and Malignant Skin Cancers with Curaderm. Drugs of Today, 26, 55-58.
Cham, B.E. and Meares, H.M. (1987) Glycoalkaloids from Solanum sodomaeum L. Are Effective in the Treatment of Skin Cancers in Man. Cancer Letters, 36, 111-118. https://doi.org/10.1016/0304-3835(87)90081-4
Cham, A., Cham, K., Chase, T. and Cham, B. (2015) A Standardized Plant Extract Containing a Target Compound Is Acceptable as a Potent Therapeutic Entity: Relevance to BEC and Solamargine, a Topical Clinical Formulation Curaderm BEC5 . Journal of Cancer Treatment and Research, 3, 22-27. https://doi.org/10.11648/j.jctr.20150302.12
Wang, Y., Gao, J., Gu, G., Li, G., Cui, C., Sun, B., et al. (2011) In Situ RBL Receptor Visualisation and Its Mediated Anticancer Activity for Solasodine Rhamnosides. ChemBioChem, 12, 2418-2420. https://doi.org/10.1002/cbic.201100551
An, W., Lai, H., Zhang, Y., Liu, M., Lin, X. and Cao, S. (2019) Apoptotic Pathway as the Therapeutic Target for Anticancer Traditional Chinese Medicines. Frontiers in Pharmacology, 10, 758. https://doi.org/10.3389/fphar.2019.00758
Li, X., Zhao, Y., Wu, W.K., Liu, S., Cui, M. and Lou, H. (2011) Solamargine Induces Apoptosis with p53 Transcription-Dependent and Transcription-Independent Pathways in Human Osteosarcoma U2OS Cells. Life Sciences, 88, 314-321. https://doi.org/10.1016/j.lfs.2010.12.006
Al Sinani, S.S., Eltayeb, E.A., Coomber, B.L. and Adham, S.A. (2016) Solamargine Triggers Cellular Necrosis Selectively in Different Types of Human Melanoma Cancer Cells through Extrinsic Lysosomal Mitochondrial Death Pathways. Cancer Cell International, 16, 287-294. https://doi.org/10.1186/s12935-016-0287-4
Zang, X., Yan, Z., Xu, T., An, Z., Chen, W., Wang, X., Huang, M. and Zhu, F. (2018) SM Derived from Solanum nigrum Induces Apoptosis of Human Cholangio Carcinoma QBC939 Cells. Oncology Letters, 15, 6329-6335. https://doi.org/10.3892/ol.2018.8171
Al Sinani, S.S.S. and Eltayeb, E.A. (2017) The Steroidal Glycoalkaloids Solamargine and Solasonine in Solanum Plants. South African Journal of Botany, 112, 253-269. https://doi.org/10.1016/j.sajb.2017.06.002
Kalalinia, F. and Korimi-Sani, I. (2017) Anticancer Properties of Solamargine: A Systematic Review. Phytotherapy Research, 31, 858-870. https://doi.org/10.1002/ptr.5809
Fu, R., Wang, X., Hu, Y., Du, H., Deng, B., Ao, S., Zhang, L., Sun, Z., Zhang, L., Lv, G. and Ji, J. (2019) Solamargine Inhibits Gastric Cancer Progression by Regulating the Expression of nc NEAT 1-2 via the MAPK Signaling Pathway. International Journal of Oncology, 54, 1545-1554. https://doi.org/10.3892/ijo.2019.4744
Sani, I.K., Marashi, S.H. and Kalalinia, F. (2015) Solamargine Inhibits Migration and Invasion of Human Hepatocellular Carcinoma Cells through Down-Regulation of Matrix Metalloproteinases 2 and 9 Expression and Activity. Toxicology in Vitro, 29, 893-900. https://doi.org/10.1016/j.tiv.2015.03.012
Cham, B.E. (2013) Inspired by Nature, Proven by Science. The New Generation Cancer Treatment That Causes Cancer Cells to Commit Suicide. Colorite Graphics Book, 264 p.
Wu, J., Tang, X., Ma, C., Shi, Y., Wu, W. and Hann, S. (2020) The Regulation and Interaction of Colon Cancer-Associated Transcript-1 and miR7-5p Contribute to the Inhibition of SP1 Expression by Solamargine in Human Nasopharyngeal Carcinoma Cells. Phytotherapy Research, 34, 201-213. https://doi.org/10.1002/ptr.6555
Shiu, L.Y., Chang, L.C., Liang, C.H., Huang, Y.S., Sheu, H.M. and Kuo, K.W. (2007) Solamargine Induces Apoptosis and Sensitizes Breast Cancer Cells to Cisplatin. Food and Chemical Toxicology, 45, 2155-2164. https://doi.org/10.1016/j.fct.2007.05.009
Sun, L., Zhao, Y., Li, X., Yuan, H., Cheng, A. and Lou, H. (2010) A Lysosomal-Mitochondrial Death Pathway Is Induced by Solamargine in Human K562 Leukemia Cells. Toxicology in Vitro, 24, 1504-1511. https://doi.org/10.1016/j.tiv.2010.07.013
Millward, M., Powell, A., Tyson, S., Daly, P., Ferguson, R. and Carter, S. (2005) Phase 1 Trial of Coramsine (SBP002) in Patient with Advanced Solid Tumors. Journal of Clinical Oncology, 23, 3105. https://doi.org/10.1200/jco.2005.23.16_suppl.3105
Munari, C.C., de Oliveira, P.F., Campos, J.C.L., Martins, S.P.L., Da Costa, J.C., Bastos, J.K. and Tavares, D.C. (2014) Antiproliferative Activity of Solanum lycocarpum Alkaloidal Extract and Their Constituents, Solamargine and Solasonine, in Tumor Cell Lines. Journal of Natural Medicines, 68, 236-241. https://doi.org/10.1007/s11418-013-0757-0
Cham, B.E. (2020) Combination Treatment with BEC and Cisplatin Synergistically Augments Anticancer Activity and Results in Increased Absolute Survival. Journal of Cancer Therapy, 11, 470-482. https://doi.org/10.4236/jct.2020.118040
Cham, B.E. (2013) Drug Therapy: Solamargine and Other Solasodine Rhamnosyl Glycosides as Anticancer Agents. Modern Chemotherapy, 2, 33-49. https://doi.org/10.4236/mc.2013.22005
Cham, B.E., Daunter, B. and Evans, R. (1991) Topical Treatment of Malignant and Premalignant Skin Cancers by Very Low Concentrations of a Standard Mixture of Solasodine Glycosides. Cancer Letters, 59, 183-192. https://doi.org/10.1016/0304-3835(91)90140-D
Chase, T.R. (2011) Curaderm BEC5 for Skin Cancers, Is It? An Overview. Journal of Cancer Therapy, 2, 728-745. https://doi.org/10.4236/jct.2011.25099
Batsev, A.F., Dobrokhotova, V.Z. and Cham, B.E. (2016) Topical Cream Curaderm BEC5 Treats a Recalcitrant Basal Cell Carcinoma. Clinical Medical Reviews and Case Reports, 3, 098. https://doi.org/10.23937/2378-3656/1410098
Goldberg, L.H., Landau, J.M., Moody, M.N. and Vergilis-Kalner, I.J. (2011) Treatment of Bowen’s Disease on the Penis with Low Concentrations of a Standard Mixture of Solasodine Glycosides and Liquid Nitrogen. Dermatologic Surgery, 37, 858-861. https://doi.org/10.1111/j.1524-4725.2011.02014.x
Punjabi, S., Cook, L.J., Kersey, P., Marks, R. and Cerio, R. (2008) Solasodine Glycoalkaloids: A Novel Topical Therapy for Basal Cell Carcinoma. A Double Blind, Randomized, Placebo-Controlled, Parallel Group, Multicentre Study. International Journal of Dermatology, 47, 78-82. https://doi.org/10.1111/j.1365-4632.2007.03363.x
Jones, P.G. and Fredrick, G.R. (1981) The Glycoalkaloid Content of Some Edible Solanaceous Fruits and Potato Products. Journal of the Science of Food and Agriculture, 32, 419-421. https://doi.org/10.1002/jsfa.2740320418
Cham, B.E., Chase, T.R. and Cham, K.E. (2020) Glycoalkaloid Combinations and Various Uses Thereof. Patent PCT/AU2017/050188.
Cham, B.E. (2019) Solasodine Glycosides from the Eggplant in a Topical Cream Psorend BEC Are Effective against Psoriasis. International Journal of Clinical Medicine, 10, 174-182. https://doi.org/10.4236/ijcm.2019.103017
Cham, B.E. (2020) Curaderm, the Undaunted New Kid on the Block for Treating Skin Cancer.
Beyer, V. and Wolverton, S.E. (2010) Recent Trends in Systemic Psoriasis Treatment Costs. Archives of Dermatology, 46, 46-54. https://doi.org/10.1001/archdermatol.2009.319
Cham, B.E. (2015) Curaderm BEC5 , Natural Non-Invasive Medication for Skin Cancer. Biopsy and 5 Year Cancer-Free Criteria. 2nd Edition, Curaderm Global Ltd., Port Vila.