Resistance of <i>Klebsiella</i> to Imipenem by Production of Carbapenemase Gene <i>bla</i><i><sub>IMP</sub></i> at Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso — Oak Academic Publishing
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Resistance of <i>Klebsiella</i> to Imipenem by Production of Carbapenemase Gene <i>bla</i><i><sub>IMP</sub></i> at Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso
Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE), UFR-SVT, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso
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Université Norbert Zongo, Centre Universitaire de Manga, Koudougou, Burkina Faso
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Département de Biochimie-Microbiologie, Université de Dédougou, Dédougou, Burkina Faso
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Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
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Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
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Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
1 Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE), UFR-SVT, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso
2 Université Norbert Zongo, Centre Universitaire de Manga, Koudougou, Burkina Faso
3 Département de Biochimie-Microbiologie, Université de Dédougou, Dédougou, Burkina Faso
4 Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
5 Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
6 Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β -lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene bla IMP in the genus Klebsiella at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. Methods: The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the Mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. Results: The antibiotic susceptibility test showed high resistance of the 17 Klebsiella isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with bla IMP -type gene. Conclusion: Klebsiella is a well-known bacterium in clinical practice. The present study demonstrated the bla IMP -type gene in cephalosporin-resistant strains of Klebsiella at CHUP-CDG. More effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.
Podschun, R. and Ullmann, U. (1998) Klebsiella spp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors. Clinical Microbiology Reviews, 11, 589-603. https://doi.org/10.1128/CMR.11.4.589
Herridge, W.P., Shibu, P., O’Shea, J., Brook, T.C. and Hoyles, L. (2020) Bacteriophages of Klebsiella spp., Their Diversity and Potential Therapeutic Uses. Journal of Medical Microbiology, 19, 176-194.
Broberg, C.A., Palacios, M. and Miller, V.L. (2014) Klebsiella: A Long way to Go towards Understanding This Enigmatic Jet-Setter. F1000Prime Reports, 6, Article No. 64. https://doi.org/10.12703/P6-64
CA-SFM-EUCAST (2021) Comité de l’antibiogramme de la Société Française de Microbiologie. https://www.sfm-microbiologie.org/wp-content/uploads/2021/04/CASFM2021__V1.0.AVRIL_2021.pdf
Hall, B.G. and Barlow, M. (2005) Revised Ambler Classification of β-Lactamases. Journal of Antimicrobial Chemotherapy, 55, 1050-1051. https://doi.org/10.1093/jac/dki130
Bush, K. and Jacoby, G.A. (2010) Updated Functional Classification of β-Lactamases. Antimicrobial Agents and Chemotherapy, 54, 969-976. https://doi.org/10.1128/AAC.01009-09
Tzouvelekis, L.S., Markogiannakis, A., Psichogiou, M., Tassios, P.T. and Daikos, G.L. (2012) Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: An Evolving Crisis of Global Dimensions. Clinical Microbiology Reviews, 25, 682-707. https://doi.org/10.1128/CMR.05035-11
Cantón, R., Akóva, M., Carmeli, Y., Giske, C.G., Glupczynski, Y., Gniadkowski, M., Livermore, D.M., Miriagou, V., Naas, T., Rossolini, G.M., Samuelsen, Ø., Seifert, H., Woodford, N. and Nordmann, P. (2012) Rapid Evolution and Spread of Carbapenemases among Enterobacteriaceae in Europe. Clinical Microbiology and Infection, 18, 413-431. https://doi.org/10.1111/j.1469-0691.2012.03821.x
Lascols, C., Peirano, G., Hackel, M., Laupland, K.B. and Pitout, J.D.D. (2013) Surveillance and Molecular Epidemiology of Klebsiella pneumoniae Isolates That Produce Carbapenemases: First Report of OXA-48-Like Enzymes in North America. Antimicrobial Agents and Chemotherapy, 57, 130-136. https://doi.org/10.1128/AAC.01686-12
Manenzhe, R.I., Zar, H.J., Nicol, M.P. and Kaba, M. (2015) The Spread of Carbapenemase-Producing Bacteria in Africa: A Systematic Review. Journal of Antimicrobial Chemotherapy, 70, 23-40. https://doi.org/10.1093/jac/dku356
Dembélé, R., Soulama, I., Kaboré, W.A.D., Konaté, A., Kagambèga, A., N’Golo, D.C., Traoré, O., Seck, A., Traoré, A.S., Guessennd, N.K., Gassama-Sow, A. and Barro, N. (2021) Molecular Characterization of Carbapenemase-Producing Enterobacteralesin Children with Diarrhea in Rural Burkina Faso. Journal of Drug Delivery and Therapeutics, 11, 84-92. https://doi.org/10.22270/jddt.v11i1.4513
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Mètuor, A. (2014) Caractérisations moléculaire et cinétique des types de β-lactamases à spectre élargi (BLSE) de souches bactériennes collectées au Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle (CHUP-CDG) de Ouagadougou. Université de Ouagadougou, Ouagadougou.
Mètuor, D.A., Tiemtoré, R.Y.W.-K., Bangré, Y.A., Zohoncon, T.M., Sougué, S., Zongo, J.K. and Simporé, J. (2019) Detection of Multidrug-Resistant Enterobacteria Simultaneously Producing Extended-Spectrum Î 2 -Lactamases of the PER and GES Types Isolated at Saint Camille Hospital Center, Ouagadougou, Burkina Faso. African Journal of Microbiology Research, 13, 414-420. https://doi.org/10.5897/AJMR2019.9147
Huang, S., Dai, W., Sun, S., Zhang, X. and Zhang, L. (2012) Prevalence of Plasmid-Mediated Quinolone Resistance and Aminoglycoside Resistance Determinants among Carbapeneme Non-Susceptible Enterobacter cloacae. PLOS ONE, 7, e47636. https://doi.org/10.1371/journal.pone.0047636
Walsh, T.R., Toleman, M.A., Poirel, L. and Nordmann, P. (2005) Metallo-β-Lactamases: The Quiet before the Storm? Clinical Microbiology Reviews, 18, 306-325. https://doi.org/10.1128/CMR.18.2.306-325.2005
Pitout, J.D.D., Nordmann, P. and Poirel, L. (2015) Carbapenemase-Producing Klebsiella pneumoniae, a Key Pathogen Set for Global Nosocomial Dominance. Antimicrobial Agents and Chemotherapy, 59, 5873-5884. https://doi.org/10.1128/AAC.01019-15
Martinez, J.L. (2014) General Principles of Antibiotic Resistance in Bacteria. Drug Discovery Today, 11, 33-39. https://doi.org/10.1016/j.ddtec.2014.02.001
Ahoyo, A.T., Baba-Moussa, L., Anago, A.E., Avogbe, P., Missihoun, T.D., Loko, F., Prévost, G., Sanni, A. and Dramane, K. (2007) Incidence d’infections liées à Escherichia coli producteur de bêta lactamase à spectre élargi au Centre hospitalier départemental du Zou et Collines au Bénin. Médecine et Maladies Infectieuses, 37, 746-752. https://doi.org/10.1016/j.medmal.2007.03.004
Toudji, A.G., Djeri, B., Karou, S.D., Tigossou, S., Ameyapoh, Y. and De Souza, C. (2017) Prévalence des souches d’entérobactéries productrices de bêta-lactamases à spectre élargi isolées au Togo et de leur sensibilité aux antibiotiques. International Journal of Biological and Chemical Sciences, 11, 1165-1177. https://doi.org/10.4314/ijbcs.v11i3.19
Mètuor-Dabiré, A., Sougué, S., Tiemtoré, R.Y.W.-K., Zohoncon, T.M., Bangré, Y.A., Ouédraogo, P., Kabré, E. and Simporé, J. (2019) Coexistence between (TOHO-Type and BES-Type) Extended-Spectrum Î 2 -Lactamase Genes of Identified Enterobacteria at Saint Camille Hospital, Ouagadougou, West Africa. International Journal of Genetics and Molecular Biology, 11, 34-40. https://doi.org/10.5897/IJGMB2019.0181
Aminul, P., Anwar, S., Molla, M.M.A. and Miah, M.R.A. (2021) Evaluation of Antibiotic Resistance Patterns in Clinical Isolates of Klebsiella pneumoniae in Bangladesh. Biosafety and Health, 3, 301-306. https://doi.org/10.1016/j.bsheal.2021.11.001
Barakzahi, M., Hormozi, B., Rashki, A. and Rashki Ghalehnoo, Z. (2014) Prevalence of Extended Spectrum β-Lactamase in Klebsiella pneumonia Isolates in a Teaching Hospital of Zahedan City, Iran. Avicenna Journal of Clinical Microbiology and Infection, 1, Article No. 22934. https://doi.org/10.17795/ajcmi-22934
Harbottle, H., Thakur, S., Zhao, S. and White, D.G. (2006) Genetics of Antimicrobial Resistance. Animal Biotechnology, 17, 111-124. https://doi.org/10.1080/10495390600957092
Muylaert, A. and Mainil, J. (2013) Résistance bactériennes aux antibiotiques, les mécanismes et leur “contagiosité”. Annales de Médecine Vétérinaire, 156, 109-123.
Shashwati, N., Kiran, T. and Dhanvijay, A.G. (2014) Study of Extended Spectrum β-Lactamase Producing Enterobacteriaceae and Antibiotic Coresistance in a Tertiary Care Teaching Hospital. Journal of Natural Science, Biology and Medicine, 5, 30-35.
Teklu, D.S., Negeri, A.A., Legese, M.H., Bedada, T.L., Woldemariam, H.K. and Tullu, K.D. (2019) Extended-Spectrum Beta-Lactamase Production and Multi-Drug Resistance among Enterobacteriaceae Isolated in Addis Ababa, Ethiopia. Antimicrobial Resistance & Infection Control, 8, Article No. 39. https://doi.org/10.1186/s13756-019-0488-4
Alizadeh, H., Khodavandi, A., Alizadeh, F. and Bahador, N. (2021) Molecular Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates Producing blaVIM, blaNDM, and blaIMP in Clinical Centers in Isfahan, Iran. Jundishapur Journal of Microbiology, 14, e114473. https://doi.org/10.5812/jjm.114473
Nordmann, P., Naas, T. and Poirel, L. (2011) Global Spread of Carbapenemase-Producing Enterobacteriaceae. Emerging Infectious Diseases, 17, 1791-1798. https://doi.org/10.3201/eid1710.110655
Gao, H., Liu, Y., Wang, R., Wang, Q., Jin, L. and Wang, H. (2020) The Transferability and Evolution of NDM-1 and KPC-2 Co-Producing Klebsiella pneumoniae from Clinical Settings. EBioMedicine, 51, Article ID: 102599. https://doi.org/10.1016/j.ebiom.2019.102599
Munita, J.M. and Arias, C.A. (2016) Mechanisms of Antibiotic Resistance. Microbiology Spectrum, 4. https://doi.org/10.1128/microbiolspec.VMBF-0016-2015
Khodadadian, R., Rahdar, H.A., Javadi, A., Safari, M. and Khorshidi, A. (2018) Detection of VIM-1 and IMP-1 Genes in Klebsiella pneumoniae and Relationship with Biofilm Formation. Microbial Pathogenesis, 115, 25-30. https://doi.org/10.1016/j.micpath.2017.12.036
Limbago, B.M., Rasheed, J.K. anderson, K.F., Zhu, W., Kitchel, B., Watz, N., Munro, S., Gans, H., Banaei, N. and Kallen, A.J. (2011) IMP-Producing Carbapenem-Resistant Klebsiella pneumoniae in the United States. Journal of Clinical Microbiology, 49, 4239-4245. https://doi.org/10.1128/JCM.05297-11
Han, R., Shi, Q., Wu, S., Yin, D., Peng, M., Dong, D., Zheng, Y., Guo, Y., Zhang, R. and Hu, F. (2020) Dissemination of Carbapenemases (KPC, NDM, OXA-48, IMP, and VIM) among Carbapenem-Resistant Enterobacteriaceae Isolated from Adult and Children Patients in China. Frontiers in Cellular and Infection Microbiology, 10, Article 314. https://doi.org/10.3389/fcimb.2020.00314
Xu, J., Lin, W., Chen, Y. and He, F. (2020) Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China. Infection and Drug Resistance, 13, 773-779. https://doi.org/10.2147/IDR.S247341
Lincopan, N., McCulloch, J.A., Reinert, C., Cassettari, V.C., Gales, A.C. and Mamizuka, E.M. (2005) First Isolation of Metallo-β-Lactamase-Producing Multiresistant Klebsiella pneumoniae from a Patient in Brazil. Journal of Clinical Microbiology, 43, 516-519. https://doi.org/10.1128/JCM.43.1.516-519.2005
Fukigai, S., Alba, J., Kimura, S., Iida, T., Nishikura, N., Ishii, Y. and Yamaguchi, K. (2007) Nosocomial Outbreak of Genetically Related IMP-1 β-Lactamase-Producing Klebsiella pneumoniae in a General Hospital in Japan. International Journal of Antimicrobial Agents, 29, 306-310. https://doi.org/10.1016/j.ijantimicag.2006.10.011
Wang, J., Yuan, M., Chen, H., Chen, X., Jia, Y., Zhu, X., Bai, L., Bai, X., Fanning, S., Lu, J. and Li, J. (2017) First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases. Antimicrobial Agents and Chemotherapy, 61, e00877-17. https://doi.org/10.1128/AAC.00877-17
Stewart, J., Judd, L.M., Jenney, A., Holt, K.E., Wyres, K.L. and Hawkey, J. (2022) Epidemiology and Genomic Analysis of Klebsiella oxytoca from a Single Hospital Network in Australia. BMC Infectious Diseases, 22, Article No. 704. https://doi.org/10.1186/s12879-022-07687-7
Mushi, M.F., Mshana, S.E., Imirzalioglu, C. and Bwanga, F. (2014) Carbapenemase Genes among Multidrug Resistant Gram Negative Clinical Isolates from a Tertiary Hospital in Mwanza, Tanzania. BioMed Research International, 2014, Article ID: 303104. https://doi.org/10.1155/2014/303104
Zafer, M.M., Al-Agamy, M.H., El-Mahallawy, H.A., Amin, M.A. and Ashour, M.S.E.-D. (2014) Antimicrobial Resistance Pattern and Their Beta-Lactamase Encoding Genes among Pseudomonas aeruginosa Strains Isolated from Cancer Patients. BioMed Research International, 2014, Article ID: 101635. https://doi.org/10.1155/2014/101635
Aghamiri, S., Amirmozafari, N., Fallah Mehrabadi, J., Fouladtan, B. and Samadi Kafil, H. (2014) Antibiotic Resistance Pattern and Evaluation of Metallo-Beta Lactamase Genes Including bla-IMP and bla-VIM Types in Pseudomonas aeruginosa Isolated from Patients in Tehran Hospitals. International Scholarly Research Notices, 2014, Article ID: 941507. https://doi.org/10.1155/2014/941507
Adam, M.A. and Elhag, W.I. (2018) Prevalence of Metallo-β-Lactamase Acquired Genes among Carbapenems Susceptible and Resistant Gram-Negative Clinical Isolates Using Multiplex PCR, Khartoum Hospitals, Khartoum Sudan. BMC Infectious Diseases, 18, Article No. 668. https://doi.org/10.1186/s12879-018-3581-z
Nordmann, P. (2014) Carbapenemase-Producing Enterobacteriaceae: Overview of a Major Public Health Challenge. Médecine et Maladies Infectieuses, 44, 51-56. https://doi.org/10.1016/j.medmal.2013.11.007