Objective: To determine the association of apoptosis in the layers of human fetal membranes distal to rupture site with labor at term. Study Design: Fetal membranes were collected from elective cesarean sections (n = 8) and spontaneous vaginal deliveries (n = 8) at term. The extent of apoptosis within fetal membrane layers was determined using terminal deoxynucleotidyl transferase deoxy-UTP-nick end labeling (TUNEL) assay and western blots for pro-apoptotic active caspase-3 and anti-apoptotic Bcl-2. Results: Apoptotic index in chorionic trophoblasts of membranes distal to rupture site obtained after vaginal delivery was 3-fold higher than those obtained from elective cesarean (11.57% ± 4.98% and 4.05% ± 2.4% respectively; p = 0.012). The choriodecidua layers after vaginal deliveries had higher expression of the pro-apoptotic active caspase-3 and less expression of the anti-apoptotic Bcl-2 than those obtained from elective cesarean sections. Conclusions: Labor at term is associated with increased apoptosis in chorionic trophoblast cells of human fetal membranes distal to rupture site.
T. M. Malak and S. Bell, “Structural Characteristics of Term Human Fetal Membranes: A Novel Zone of Extreme Morphological Alteration within the Rupture Site,” BJOG: An International Journal of Obstetrics & Gynaecology, Vol. 101, No. 5, 1994, pp. 375-386.
L. Myatt and K. Sun, “Role of Fetal Membranes in Signaling of Fetal Maturation and Parturition,” The International Journal of Developmental Biology, Vol. 54, No. 2-3, 2010, pp. 545-553. doi:10.1387/ijdb.082771lm
W. L. Whittle, F. A. Patel, N. Alfaidy, A. C. Holloway, M. Fraser, S. Gyomorey, S. J. Lye, W. Gibb and J. R. Challis, “Glucocorticoid Regulation of Human and Ovine Parturition: The Relationship between Fetal Hypothalamic-Pituitary-Adrenal Axis Activation and Intrauterine Prostaglandin Production,” Biology of Reproductio, Vol. 64, 2001, pp. 1019-1032. doi:10.1095/biolreprod64.4.1019
W. Gibb, “The Role of Prostaglandins in Human Parturition,” Annals of Medicine, Vol. 30, No. 3, 1998, pp. 235-241. doi:10.3109/07853899809005850
R. M. Moore, J. M. Mansour, R. W. Redline, B. M. Mercer and J. J. Moore, “The Physiology of Fetal Membrane Rupture: Insight Gained from the Determination of Physical Properties,” Placenta, Vol. 27, No. 11-12, 2006, pp. 1037-1051. doi:10.1016/j.placenta.2006.01.002
D. L. Vaux, “Toward an Understanding of the Molecular Mechanisms of Physiological Cell Death,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 3, 1993, pp. 786-789. doi:10.1073/pnas.90.3.786
L. E. Gerschenson and R. J. Rotello, “Apoptosis: A Different Type of Cell Death,” FASEB Journal, Vol. 6, No. 7,1992; 2450-2455.
S. W. Hetts, “To Die or Not to Die: An Overview of Apoptosis and Its Role in Disease,” Japan Automobile Manufacturers Association, Vol. 279, No. 4, 1998, pp. 300-307. doi:10.1001/jama.279.4.300
W. C. Earnshaw, L. M. Martins and S. H. Kaufmann, “Mammalian Caspases: Structure, Activation, Substrates, and Functions during Apoptosis,” Annual Review of Biochemistry, Vol. 68, 1999, pp. 383-424. doi:10.1146/annurev.biochem.68.1.383
R. Runic, C. J. Lockwood, L. LaChapelle, et al., “Apoptosis and Fas Expression in Human Fetal Membranes,” Journal of Clinical Endocrinology & Metabolism, Vol. 83, 1998, pp. 660-666. doi:10.1210/jc.83.2.660
J. McLaren, D. J. Taylor and S. C. Bell, “Increased Incidence of Apoptosis in Non-Labor-Affected Cytotrophoblast Cells in Term Fetal Membranes Overlying the Cervix,” Human Reproduction, Vol. 14, No. 2, 1999, pp. 2895-2900. doi:10.1093/humrep/14.11.2895
M. El Khwad, V. Pandey, B. Stetzer, B. M. Mercer, D. Kumar, R. M. Moore, R. W. Fox, J. M. Mansour and J. J. Moore, “Fetal Membranes from Term Vaginal Deliveries Have a Zone of Weakness Exhibiting Characteristics of Apoptosis and Modeling,” Journal of the Society for Gynecologic Investigation, Vol. 13, No. 3, 2006, pp. 191-195. doi:10.1016/j.jsgi.2005.12.010
H. M. Harirah, S. E. Donia, V. Parkash, D. C. Jones and C. D. Hsu, “Localization of Fas-Fas Ligand System in Human Fetal Membranes,” Journal of Reproductive Medicine, Vol. 47, No. 8, 2002, pp. 611-616.
C. D. Hsu, S. F. Hong, H. Harirah, R. Bahado-Singh and L. C. Lu, “Amniotic Fluid sFas Levels in Intra-Amniotic Infection,” Obstetrics & Gynecology, Vol. 95, No. 5, 2000, pp. 667-670. doi:10.1016/S0029-7844(99)00664-X
M. Lappas, C. Riley, R. Menon and M. Permezel, “Expression and Localization of FoxO3 abd FoxO4 in Human Placenta and Fetal Membranes,” Placenta, Vol. 31, No. 12, 2010, pp. 1043-1050. doi:10.1016/j.placenta.2010.09.009
D. T. Loo, “In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques,” Methods in Molecular Biology, Vol. 682, 2011, pp. 3-13. doi:10.1007/978-1-60327-409-8_1
N. G. Reti, M. Lappas, C. Riley, M. E. Wlodek, M. Permezel, S. Walker and G. E. Rice, “Why Do Membranes Rupture at Term? Evidence of Increased Cellular Apoptosis in the Supracervical Fetal Membranes,” American Journal of Obstetrics & Gynecology, Vol. 196, No. 5, 2007, pp. 1-10. doi:10.1016/j.ajog.2007.01.021
A. M. Germain, J. Smith, M. L. Casey and P. C. Mac-Donald, “Human Fetal Membranes Contribution to the Prevention of Parturition: Uterotonin Degradation,” Journal of Clinical Endocrinology and Metabolism, Vol. 78, 1994, pp. 463-470. doi:10.1210/jc.78.2.463
R. K. Sangha, J. C. Walton, C. N. Ensor, et al., “Immunohistochemical Localization , Messenger Ribonucleic Acid Abundance, and Activity of 15-Hydroxyprostaglandin Dehydrogenase in Placenta And Fetal Membranes during Term and Preterm Labor,” Journal of Clinical Endocrinology and Metabolism, Vol. 78, No. 4, 1994, pp. 715-723. doi:10.1210/jc.78.4.982
T. So, A. Ito, Y. Mori and S. Hirakawa, “Tumor Necrosis Alpha Stimulates the Biosynthesis of Matrix Metalloproteinases and Plasminogen Activator in Cultured Human Chorionic Cells,” Biology of Reproduction, Vol. 46, No. 5, 1992, pp. 772-778. doi:10.1095/biolreprod46.5.772
F. Vadillo-Ortega, G. Gonzalez-Avila, E. E. Furth, et al., “92 kD-Type IV Collagenase (Matrix Metalloproteinase-9) Activity in Amniochorion Increases with Labor,” American Journal of Pathology, Vol. 146, No. 1, 1995, pp. 148-156.
C. Kakinuma, C. Kuwayama, N. Kaga et al., “Trophoblastic Apoptosis in Mice with Preterm Delivery and Its Suppression by Urinary Trypsin Inhibitor,” Obstetrics & Gynecology, Vol. 90, No. 1, 1997, pp. 117-124. doi:10.1016/S0029-7844(97)00176-2
M. L. Casey and P. C. MacDonald, “Human Parturition: Distinction between the Initiation of Parturition and the Onset of Labor,” Seminars in Reproductive Endocrinology, Vol. 11, 1993, pp. 272-284. doi:10.1055/s-2007-1018840
R. B. George, J. Kalich, B. Yonish and A. P. Murtha, “Apoptosis in the Chorion of Fetal Membranes in Preterm Premature Rupture of Membranes,” American Journal of Perinatology, Vol. 25, No. 1, 2008, pp. 29-32. doi:10.1055/s-2007-1004828