Nephroprotective Effect of <i>Nigella Sativa</i> and <i>Matricaria Chamomilla</i> in Cisplatin Induced Renal Injury—Supportive Treatments in Cisplatin Nephrotoxicity — Oak Academic Publishing
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Nephroprotective Effect of <i>Nigella Sativa</i> and <i>Matricaria Chamomilla</i> in Cisplatin Induced Renal Injury—Supportive Treatments in Cisplatin Nephrotoxicity
Nigella sativa and Matricaria chamomilla are extensively consumed as tea or tonic. Despite their widespread use as a home remedy, relatively few trials evaluated their benefits in nephroprotection. Hence, this study evaluates the nephroprotective effects of supportive treatments (N. sativa, M. chamomilla and vitamin E) in cisplatin nephrotoxicity rat model. Eighty rats divided into 10 groups, of 8 animals each. The first group (G1) injected with saline intra-pretoneal (i.p). G2 injected with 5 mg/kg cisplatin i.p on zero day of experiment and repeated 4 times, with 5 days free interval. G3-G10 received daily supportive treatments, started 5 days before the experiment (–5day). Concomitantly G4, G6, G8 and G10 injected with 5 mg/kg cisplatin i.p like G2. On day sixteen, animal scarified, serum and/or kidney tissue were used to determine kidney function tests (serum urea, creatinine, NAG, β-gal), oxidative stress indices (NO, LPO), antioxidant activities (SOD), sulphur compounds (GGT, GSH, total thiols ), apoptotic indices (cathepsin D, DNA fragmentation), two minerals (Ca<sup>2+</sup> and zn<sup>2+</sup>). Cisplatin caused marked elevation in serum GGT that reduced signifi-cantly in group received M. chamomilla with cisplatin (P < 0.001). There is a correlation between GGT and NAG in cisplatin group (r = 0.731 p < 0.05) that may suggest one of possible mechanisms of renal injury by cisplatin. M. chamomilla followed by N. sativa and vitamin E improved the biochemical and pathological renal injury, as determined by increasing the body weight, normalizing the kidney functions, decreasing the oxidative stress markers, improving the apoptotic markers, minimizing the pathological changes. Hence, N. sativa and M. chamomilla will be a promising nephroprotective agents for reducing cisplatin nephrotoxicity, most probably, by antioxidants effects and inhibition GGT production, respectively.
KeywordsCisplatinNephrotoxicityNephroprotectionMatricaria ChamomillaNigella SativaVitamin E
X. Yao, K. Panichpisal ,N. Kurtzman ,K. Nugent, “Cisplatin nephrotoxicity: a review”. Am J Med Sci., Vol. 334, No. 2, 2007, pp.115-24.
B. Shaikh, and J.Hatcher. “Complementary and alternative medicine in Pakistan: prospects and limitations”, Evid. Based Complement. Altern. Med., Vol. 2, 2005, pp 139- 142.
B. H Ali, G. Blunden, “Pharmacological and toxicological properties of Nigella sativa”, Phytother Res., Vol.17, No. 4, 2003, pp 299-305.
M. R Heidari, Z. Dadollahi , M. Mehrabani, H. Mehrabi, M. Pourzadeh-Hosseini, E. Behravan, L. Etemad, “Study of antiseizure effects of Matricaria recutita extract in mice”, Ann N Y Acad Sci., Vol.117, No.1, 2009, pp 300-4.
D. L McKay ,J.B Blumberg,” A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.)”, Phytother Res., Vol. 20, No.7; 5, 2006, pp19-30.
V. M Chandrashekhar, V. L Ranpariya, A. Parashar ,A.A Muchandi, S. Ganapaty, “Neuroprotective activity of Matricaria recutita Linn against global model of ischemia in rats”, J Ethnopharmacol., Vol. 17, No.127;3, 2010, pp 645-51.
I. Barene, I. Daberte, L. Zvirgzdina and V. Iriste, “The complex technology on products of German Chamomile” Medicina (Kaunas), Vol. 39, No.2,2003, pp 127-131.
E. El-Daly, “Protective effect of cysteine and vitamin E, Crocus sativus and Nigella sativa extracts on cisplatin- induced toxicity in rats”, J Pharm Belg. Vol. 53, No.20, 1998, pp 87-95.
H. Lipp, J. Hartmann, “Platinum compounds: metabolism, toxicity and supportive stratigies”, Schweiz, Rundsch. Med. Prax., Vol. 94, No. 6, 2005, pp 187-198.
R. Van Bezoijen, A.Ederveen, H. Kloosterboer,S. Papapoulos, C. Lowik, “Plasma nitrate and nitrite level are regulated by ovarian steroids but do not correlate with trabecular bone mineral density in rats”, J. Endocrinology, Vol. 159, 1998, pp 27-34.
W. Thayer, “Lipid peroxide in rats treated chronically with adriamycin”, Biochem. Pharmacol., Vol. 33, No. 14, 1984, pp 2259-2263.
H. Misra, I.Fridovich, “The role of super oxide ion in the oxidation of the epinephrine and a simple assay for superoxide dismutase”, J. Biol. Che., Vol. 247, No. 10, 1972, pp 3170-3187.
D. Maruhn D, “Rapid colorimetric assay by ?-Galacto- sidase and N- acetyl-? glucosaminidase in human urine”, Clinica Chimica Acta, Vol. 73, 1976, pp.453-461.
E. Beutler,O. Duron, B. Kelly, “Improved method for determination of blood glutathione”, J. Lab. Clin. Med., Vol. 61, 1963, pp 882-888.
S. Kühn and M. deKock, “A preliminary study of elevated alkaline phosphatase and cathepsin in bronchial aspirates of patients with lung cancer and bronchitis. Chest. Vol. 38, No. 3, 1975, pp 326-330.
C. Paradones, V. Illera, D. Peckham, L. Stunz, R. Ashman,” Regulation of apoptosis in vitro in mature spleen T cells”, J. Immun., Vol. 151, 1993, pp 3521-3529.
Bancroft G, Stevens L. A theory and practice of histology technique. USA. Churchili Livingston Publisher.; 2nd Ed., Vol. 5, 1982, pp 842-518.
B. Bonevski, A. Wilson, D. A Henry, “An analysis of news media coverage of complementary and alternative medicine”, PLoS ONE, Vol. 11,No. 3; 6, 2008, pp 2406
Z. Hawsawi, B. Ali, A. Bamosa, “Effect of Nigella sativa (black seed) and thymoquinone on blood glucose in albino rats”, Annals of Saudi Medicine, Vol. 21 No. 3, 4, 2001, pp 242-244.
A. Omar, S. Ghosheh, A. Abdulghani, A. Houdi A,P.A Crookscor, “High performance liquid chromatographic analysis of the pharmacologically active quinones and related compounds in the oil of the black seed (L. Nigella sativa)”, J Pharm Biomed Anal., Vol. 19, 1999, pp757-62
I. Ogata, T. Kawanai, E. Hashimoto, Y. Nishimura, Y. Oyama, H. Seo, “Bisabololoxide A, one of the main constituents in German chamomile extract, induces apoptosis in rat thymocytes”, Arch Toxicol., Vol. 84, No. 1, 2010, pp 45-52.
M. H Hanigan, E. D Lykissa, D. M Townsend, Ching- Nan Ou, et al., “Gamma Glutamyl transpeptidase-deficient mice are resistant to the nephrotoxic effects of cisplatin”, American Journal of Pathology, Vol. 159, 2001, pp 1889- 1894.
S. Saad, T. Najjar, M. Daba, A. Al-Rikabi, “Inhibition of nitric oxide synthase aggrevates cisplatin induced nephrotoxicity: effect of 2-amino-4-methylpyridine”, Chemotherapy, Vol. 48, 2002, pp 309-315.
A. Al-Majed, A. Abd-Allah A, C. Ammar, A. Al-Rikabi, O.Al-Shabanah, Mostafa A, “Effect of oral administration of Arabic gum on cisplatin-induced nephrotoxicity in rats”, J. Biochem. Molecul. Toxicol., Vol. 17, No. 3, 2003, pp146-153.
K. Weichert-Jacobsen, A. Bannowski, F. Küppers, T. Loch, M. St?ckle, “Direct amifostine effect on renal tubule cells in rat”, Cancer Res., Vol.59, 1999, pp 3451- 3453.
Y. Kawai, S. Taniuchi, S. Okahara, M .Nakamura, M. Gemba, “Relationship between Cisplatin or Nedaplatin induced nephrotoxicity and renal accumulation”, Biol. Pharm.Bull., Vol. 28, No.8, 2005, pp 1385-1388.
C. Bazzi, C. Petrini,V. Rizza, G. Arrigo, M. Paparella, P. Napodano, et al., “Urinary N-acetyl-βglusominidase excretion is a marker of tubular cell dysfunction and a predictor of out come in primary glomerulonephritis”, Nephrol. Dial. Transplant., Vol. 17, No.11, 2002, pp 1890-1896.
S. Lee, W .Kim, S. Moon, M. Sung, D .Kim, K. Kang, et al., “Protective role of L-2-oxothiazolidine-4-carboxylic acid in cisplatin-induced renal injury”, Nephrol. Dial. Transplant., Vol. 21, No.8, 2006, pp 2096-105
N. Sheena, T. Ajith, K. Janardhanan, “Prevention of nephrotoxicity induced by the anticancer drug cisplatin, using a medicinal mushroom occurring in South India”, Current Science, Vol. 85, No. 4, 2003, pp 478-482.
L. Antunes, J. Darin, M. Bianchi, “Protective effects of vitamin C against cisplatin-induced nephrotoxicity and lipid peroxidation in adult rats: a dose dependent study”, Pharmacol. Res., Vol.41, 2000, pp 405-411.
I. Hara, H. Miyake, K. Yamanaka, S. Hara, S. Kamidono, “Serum cathepsin D and its density in men with prostate cancer as new predictors of disease progression”, Oncology Reports, Vol. 9, 2002, pp 1379-1383.
A. Pompella, A. Corti, A. Paolicchi, C.Giommarelli, F. Zunino, “Gamma-glutamyltransferase, redox regulation and cancer drug resistance”, Curr. Opin. Pharmacol., Vol. 7, No.4, 2007, pp 360-6.
M. W Lieberman, A.L Wiseman, Z. Shi, B. Z Carter, R. Barrios, C. Ou, et al., “Growth retardation and cysteine deficiency in gamma-glutamyl transpeptidase-deficient mice”, Proc Natl Acad. Sci., Vol. 93, 1996, pp 7923-7926
D. Townsend, M. Deng, L. Zhang, M. Lapus, M. Hanigan, “Metabolism of cisplatin to a nephrotoxin in proximal tubule cell”, J. Am. Soc. Nephrol., Vol. 14, 2003, pp1-10.
D. Townsend, M. Hanigan, “Inhibition of gamma-glutamyl transpeptidase or cysteine S-conjugate beta-lyase activity blocks the nephrotoxicity of cisplatin in mice”, J. Pharmacol. Exp. Ther., Vol. 300, 2002, pp. 142-148.
H. Viola, C. Wasowski, M. Levi De Stein, “Apigenin, a component of Matricaria recutita flowers, is a central benzodiazepine receptors-ligand with anxiolytic effects”, Planta Medica, Vol. 61, 1995, pp 213-216.
E. Rekka, A. Kourounakis, P. Kourounakis, “Investigation of the effect of chamazulene on lipid peroxidation and free radical processes”, Res. Com. in Mol. Pathology & Pharmacology, Vol.92, 1996, pp 361-364.
M. Boskabady, S. Kiani, P. Jan daghi, T. Ziaei, A. Zarei, “Comparison of antitussive effect of Nigella sativa with codeine in guinea pig”, IJMS. Vol. 28, No. 3, 2003, pp 111-115.
M. Krych, “Acute renal failure”, Internist., Vol.46, No.1, 2005, pp. 30-38.
Y .Kawai, T .Nakao, N. Kunimura, Y. Kohada, M.Gemba, “Relationship of intracellular calcium and oxygen radicals to cisplatin related renal cell injury”, J. Pharmacol. Sci., Vol. 100, No.1, 2006, pp 65-72.
A. M. Al-Gaby “Amino acid composition and biological effects of supplementing broad bean and corn proteins with (black cumin) cake protein”, Nahrung, Vol. 290, 1998, pp 4-8
S. Joshi, S. K Hasan, R. Chandra, M. M Husain, R. C. Srivastava, “Scavenging action of zinc and green tea polyphenol on cisplatin and nickel induced nitric oxide generation and lipid peroxidation in rats”,Biomed Environ Sci., Vol. 17, No.4, 2004, pp 402-9.
M. Shimura, A .Saito, S. Matsuyama, T. Sakuma, Y. Terui, K. Ueno, “Element array by scanning X-ray fluorescence microscopy after cis-diamminedichloro - platinum(II) treatment”, Cancer Res. Vol. 65, No.12, 2005, pp 4998-5002.
Y. Liao, X. Lu, C. Lu, G. Li, Y .Jin, H. Tang. “Selection of agents for prevention of cisplatin-induced hepatotoxicity”, Pharmacol Res., Vol. 57, No. 2, 2008, pp 125-31.