The AL-SENSE Test is Reliable for Detection of Second Trimester Amniotic Fluid
- 1
- 2
- 3
- 4
- 5
Abstract
Objective: To validate the reliability and stability of the AL-SENSE panty-liner for detection of amniotic fluid in the second trimester. Study design: Amniotic fluid drawn during amniocentesis between 16 to 23 weeks of gestation was used for the study.100 µl and 400 µl of amniotic fluid, were dripped onto two panty liners, respectively. After 5 minutes the indicator strip was removed from the AL-SENSE, dried and examined. The color was recorded at time 0 and 5 minutes, and then every 10 minutes for the first 30 minutes, then at 1, 2, 6 and 12 hours. Results: 50 women were enrolled and 49 completed the study. 100% of both volume samples changed color from yellow to blue during the first 5 minutes and remained stable after twelve hours of follow-up. In the 100 μl subgroup, seven of the 49 samples (14.3%) had a delayed color change and in the 400 μl subgroup one of 49 (2.04%) had a delay in change of color. Conclusion: Amniotic fluid drawn during amniocentesis at weeks 16 - 23 of gestation have a positive, stable staining effect on the AL-SENSE panty liner. Hence, AL-SENSE may be reliable for detection of amniotic fluid leakage during the second trimester.
- Bornstein J, Geva A, Solt I, Fait V, Schoenfeld A, Shoham HK, Sobel J.Nonintrusive Diagnosis of Premature Ruptured Amniotic Membranes Using a Novel Polymer. Am J Perinat 2006; 23: 351-354.
- Bornstein J, ohel G, Sorokin Y, Reape KZ, Shmaider O, Kessary-Shoham H, Ophir E. Effectiveness of a novel home-based testing device for the detection of rupture of membranes. Am J Perinat 2009; 26: 45-50.
- Mulhair L, Carter J, Poston L, Seed P, Briley A. Prospective cohort study investigating the reliability of the AmnioSense method for detection of spontaneous rupture of membranes. BJOG 2009; 116: 313-318.
- Jauniaux E, Jurkovic D, Gulbis B, Collins WP, Zaidi J, Campbell S. Investigation of acid-base balance of coelo- mic and amniotic fluids in early human pregnancy. Am J Obstet Gynecol 1994; 170: 1365-9.
- Greenough A. and Blott M. Neonatal outcome after prolonged rupture of the membranes starting in the second trimester. Arch Dis Child 1988; 63: 1146-50.
- Farooqi A, Holmgren PA, Engberg S, Serenius F. Survival and 2-year outcome with expectant management of second-trimester rupture of membranes. Obstet Gynecol 1998; 92: 895-901.
- Hibbard JU, Hibbard MC, Ismail M, Arendt E. Pregnancy Outcome after expectant management of premature rupture of the membranes in the second trimester. J Reprod Med 1993; 38: 945-951.
- Gold RB, Goyert GL, Schwartz DB, Evans MI, Seabolt LA. Conservative management of second trimester post amniocentesis fluid leakage. Obstet Gynecol 1989; 74: 745-747.
- Borgida AF, Mills AA, Feldman DM, Rodis JF, Egan JFX. Outcome of pregnancies complicated by ruptured membranes after genetic amniocentesis. Am J Obstet Gynecol 2000; 183:937-939.