Influence on Time to Cryopreservation in Umbilical Cord Blood Units
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
Abstract
It is accepted that the Umbilical Cord Blood (UCB) units can be cryopreserved to 48 h after their harvest and the delay in this procedure influences the final quality. The aim of this study was to determine the influence on time to cryopreservation in Umbilical Cord Blood Units. A selection of the 2134 samples arrived to the UCB Bank from 36 Andalusian linked hospitals was analyzed for a period of six months. The date and hour of collection, hospital of origin, time in the hospital, time of transport, time of arrival to the bank to cryopreservation, viability and CD34+ cells were determined. Our results showed that the median time from harvest to cryopreservation was 27.9 h. However, a great variability was found (range: 5.3 to 47.6 h), which was dependent on transport variables: time from collection to leave the hospital (median 12.6 h, range: 0.1 - 28.8), time of transport from hospital to UCB Bank (median 1.8 h, range: 0.1 - 20.6) and time kept in the bank before freezing. Viability of cells were reduced directly proportional in samples that took longer to be cryopreserved (93.9 vs 96.6%, p = 0.001), by contrast, CD34+ count did not differ significant at shorter or longer times of cryopreservation. In conclusion, the delay in transport time and in processing reduce the umbilical cord units quality, consequently, the time of transport and storage previous to cryopreservation must be reduced less than 36 h.
- F. Beaujean, J. Pico, F. Norol, M. Divine, C. Le Forestier and N. Duedari, “Characteristics of Peripheral Blood Progenitor Cells Frozen after 24 h of Liquid Storage,” Journal of Hematotherapy, Vol. 5, No. 6, 1996, pp. 681- 686. doi:10.1089/scd.1.1996.5.681
- U. F. Koenigbauer, S. R. Burger and J. McCullough, “Non-Frozen Preservation of Umbilical Cord Blood,” Transfusion, Vol. 42, No. 10, 2002, pp. 1383-1384. doi:10.1046/j.1537-2995.2002.00259.x
- A. Hubel, D. Carlquist, M. Clay and J. McCullough, “Short-Term Liquid Storage of Umbilical Cord Blood,” Transfusion, Vol. 43, No. 5, 2003, pp. 626-632.
- S. J. Fasouliotis and J. G. Schenker, “Human Umbilical Cord Blood Banking and Transplantation: A State of the Art,” European Journal of Obstetrics & Gynecology and Reproductive Biology, Vol. 90, No. 2000, pp. 13-25.
- K. J. Moise, “Umbilical Cord Stem Cells,” Obstetrics & Gynecology, Vol. 106, No. 6, 2005, pp 1393-1407. doi:10.1097/01.AOG.0000188388.84901.e4
- P. Solves, F. Carbonell-Uberos, V. Mirabet, R. Roig, “CD34+ Cell Content for Selecting Umbilical Cord Blood Units for Cryopreservation,” Transfusion, Vol. 47, No. 3, 2007, pp. 552-553. doi:10.1111/j.1537-2995.2006.01149.x
- M. Eapen, P. Rubinstein, M. J. Zhang, C. Stevens, J. Kurtzberg, A. Scaradavou, F. R. Loberiza, et al., “Outcomes of Transplantation of Unrelated Donor Umbilical Cord Blood and Bone Marrow in Children with Acute Leukaemia: A Comparison Study,” Lancet, Vol. 369, No. 9577, 2007, pp. 1947-1954.
- M. J. Sullivan, “Banking on Cord Blood Stem Cells,” Nature Reviews Cancer, Vol. 8, No. 7, 2008, pp. 254-263. doi:10.1038/nrc2418
- NETCORD FACT, “International Standards for Cord Blood Collection, Processing, Testing, Banking, Selection and Release,” 3rd Edition, FAHCT Accreditation Office, Omaha, 2006.
- Comité de Acreditación en Transfusión (CAT), “Estándares de Acreditación en Obtención, Procesamiento, Almacenamiento y Distribución de Progenitores Hematopoyéticos de cordón Umbilical,” 3rd Edition, Grupo Acción Médica, Madrid, 2007.
- J. E. Wagner, J. N. Barker, T. E. DeFor, K. S. Baker, B. R. Blazar, C. Eide, A. Goldman, et al., “Transplantation of Unrelated Donor Umbilical Cord Blood in 102 Patients with Malignant and Nonmalignant Diseases: Influence of CD34 Cell Dose and HLA Disparity on Treatment-Related Mortality and Survival,” Blood, Vol. 100, No. 5, 2002, pp. 1611-1618.
- S. Terakura, E. Azuma, M. Murata, et al., “Hematopoietic Engraftment in Recipients of Unrelated Donor Umbilical Cord Blood is Affected by the CD34+ and CD8+ cell Doses,” Biology of Blood and Marrow Transplantation, Vol. 13, No.7, 2007, pp. 822-830. doi:10.1016/j.bbmt.2007.03.006