A novel mediatorless reusable glucose biosensor with a remarkable shelf life has been fabricated on electrodeposited film of chemically synthesized nanostructured polyaniline (NSPANI) on indium tin oxide (ITO) coated glass plates using cyclic voltammetry. Glucose oxidase has been covalently immobilized on electrodeposited NSPANI film to fabricate a glucose bioelectrode (GOx/NSPANI-SDS/ITO). The results of linear sweep voltammetry and the high value of heterogeneous rate constant as obtained using Laviron equation indicates that GOx/NSPANI-SDS/ITO bioelectrode can detect glucose in the range of 0.5 to 10.00 mM with high sensitivity of 13.9 μ A?mM ?1 with a fast response time of 12 seconds. The linear regression analysis of bioelectrode reveals standard deviation and correlation coefficient of 6 μ A and 0.994, respectively. The low value of Michaelis - Menten constant (K m ) estimated as 0.28 mM using Lineweaver - Burke plot indicates high affinity of glucose oxidase enzyme to glucose and transfer rate. The GOx/NSPANI-SDS/ITO bioelectrode exhibits uniform activity for 12 weeks under refrigerated conditions; however the study is further going on. Attempts have been made to utilize this electrode for estimation of glucose in blood serum and results are found to be within 11% error. The unique features of this novel electrode lie on its reusability, real time monitoring, reproducibility and remarkable shelf life apart from the wide linear range, high sensitivity, low K m value, high heterogeneous electron- transfer constant etc .
KeywordsGlucose BiosensorNanostructured PolyanilineReusabilityReal Time Monitoring and Electrodeposition
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