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A Fluorescence Ratiometric Probe for Cysteine/Homocysteine and Its Application for Living Cell Imaging
Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China
Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China
Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China
- 1 Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China
- 2 Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China
- 3 Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China
International Journal of Organic Chemistry·Volume 03 (2013)·Pages 235–239·Published 4 December 2013·DOI10.4236/ijoc.2013.34033
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Abstract
A fluorescence ratiometric probe 1 for cysteine (Cys) and homocysteine (Hcy) has been rationally constructed based on intramolecular charge transfer (ICT) mechanism. Upon treatment with Cys/Hcy, probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength displaying a large shift (from 526 nm to 446 nm). When 90 μM Cys were added, the emission ratios (I 446 /I 526 ) of the probe changed dramatically from 0.01797 to 4.65472. The detection limit was also measured to be 0.18 μM ( S / N = 3). The theoretical calculations have confirmed that the ratiometric response of probe 1 to Cys/Hcy is due to the inhibition of ICT process upon the reaction of probe 1 with Cys/Hcy. Furthermore, the fluorescence imaging experiments in living cell demonstrated that probe 1 was favourable for intracellular Cys/Hcy imaging.
KeywordsFluorescent ProbesFluorescenceCysteine and HomocysteineFluorescence Imaging
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