Research ArticleOpen AccessGoogle Scholar indexed
Determination of Sedative Component in Chinese Medicines by High Resolution Chromatography-Electron Impact-Mass Spectrometry
State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, China
State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, China
Shanxi northern Xingan Chemical Industry Co.Ltd
State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, China
- 1 State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, China
- 2 State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, China
- 3 Shanxi northern Xingan Chemical Industry Co.Ltd
- 4 State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, China
Journal of Analytical Sciences, Methods and Instrumentation·Volume 02 (2012)·Pages 13–17·Published 13 March 2012·DOI10.4236/jasmi.2012.21003
Copy link · social · email
Abstract
The sedative compounds in two traditional Chinese medicines were studied. These compounds are present in the medicinal herbs “Zao Ren” and “Lu Lu Tong”. The samples were first extracted by ethanol and pre-treated by ultrasonic wave, and then they were separated and qualitatively analyzed by high-resolution GC-EI-MS and High Performance Liquid Chromatography (HPLC) systems. We have found that the Chinese herb medicines “Zao Ren” contained clonazepam (120 ppm), whereas “Lu Lu Tong” Chinese drug contained estazolam (270 ppm). The Analytical measurements were done by using three different diagnostic indices using the GC-retention time of the GC-EI-MS total –ion–chromatogram; single stage EI-MS analysis identification and from the measurements of the retention time in the HPLC.
KeywordsChinese MedicineGC-EI-MSHPLCClonazepamEstazolam
- Y. X. Xu, “Neuroses,” Peking University Medical Press, Beijing, 2008, pp. 2-3.
- X. J. Li, Y. P. Zhang and Z. B. Yuan, “Separation and Determination of Rutin and Vitamin C in Compound Rutin Tablets by Capillary Electrophoresis with Ampero-metric Detection,” Chinese Journal of Analytical Chemistry, Vol. 30, No. 7, 2002, pp. 815-818.
- K. D. Yang, J. Chen, Z. Ouyang, W. D. Xu and Z. F. Tong, “Analysis of Polyprenols in Ginkgo Biloba Leaves with High Performance Liquid Chromatography PAtmospheric Pressure Chemical Ionization Mass Spectrometry,” Chinese Journal of Analytical Chemistry, Vol. 31, No. 8, 2003, pp. 937-940.
- Q. Li, G. L. Hu, X. G. Xu, Z. X. Zhuang and X. R. Wang, “Determination of Andrographolide and Dehydroandrographolide in Andrographis Paniculata Ness by Micellar Electrokinetic Capillary Chromatography,” Chinese Journal of Analytical Chemistry, Vol. 31, No. 4, 2003, pp. 451-454.
- Z. Zeng, J. G. Shi, H. P. Zeng and W. L. Lai, “Application of Organic Mass Spectrometry in Studies on Houttuynia Cordata,” Chinese Journal of Analytical Chemistry, Vol. 31, No. 4, 2003, pp. 399-404.
- X. Y. Shang and Z. B. Yuan, “Determination of Effective Constituents in Rhubarb by β2Cyclodextrin Modified Micellar Electrokinetic Chromatography,” Chinese Journal of Analytical Chemistry, Vol. 30, No. 7, 2002, pp. 853-856.
- L. Wang, H. B. Xiao, J. H. Hu, J. Wang and X. M. Liang, “Study of p2Hydroxyphenylic Compounds in Gastrodia Elata Blume by High Performance Liquid Chromatography Electrospray Mass Spectrometry,” Chinese Journal of Analytical Chemistry, Vol. 31, No. 8, 2003, pp. 954-957.
- Q. A. Wang, H. Z. Guo, C. H. Huo, Q. W. Shi, M. Ye, K. S. Bi and D. A. Guo, “Chemical Constituents in Root of Paeonia Lactiflora,” Chinese Traditional and Herbal Drugs, Vol. 38, No. 7, 2007, pp. 972-976.
- N. X. Zhao, Y. M. Han and X. L. Fu, “Chemical Constituents in Stem of Lonicera Japonica,” Chinese Traditional and Herbal Drugs, Vol. 38, No. 12, 2007, pp. 1774-1776.
- Z. F. Fang, Z. L. Li, Y. Wang, W. Li and H. M. Hua, “Chemical Constituents from Wing Twigs of Euonymus Alatus,” Chinese Traditional and Herbal Drugs, Vol. 38, No. 6, 2007, pp. 810-812.
- D. Lu, C. Y. Wang, J. P. Liu, S. A. Chen and P. Y. Li “Chemical Constituents from Aerial parts of Dioscorea Nipponica,” Chinese Traditional and Herbal Drugs, Vol. 38, No. 12, 2007, pp. 1785-1787.
- S. Que, Y. S. Zhang and Y. Y. Zhao, “Chemical Constituents of Tibetan Medicine Oxytropis Falcate,” Chinese Traditional and Herbal Drugs, Vol. 38, No. 10, 2007, pp. 1458-1460.