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Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer
Faculty of Physics, University of Regensburg, Regensburg, Germany.
- 1 Faculty of Physics, University of Regensburg, Regensburg, Germany.
Journal of Analytical Sciences, Methods and Instrumentation·Volume 03 (2013)·Pages 234–239·Published 8 October 2013·DOI10.4236/jasmi.2013.34030
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Abstract
In the sample compartment of a conventional spectrophotometer, mounting of a polarizer before sample and an analyzer behind sample allows the determination of the optical rotatory dispersion of optical active media by measurement of the transmission ratio of crossed and parallel arranged polarizer and analyzer. A formula for the determination of the angle of rotation is derived from the transmission ratio. The arrangement is applied to determine the molar optical rotation of D-glucose in water in the wavelength range from 220 nm to 820 nm.
KeywordsOptical ActivityOptical Rotatory DispersionSpecific Optical RotationMolar Optical RotationPolarimeterSpectropolarimeterGrape SugarD-Glucose
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