The present study compared intracytoplasmic inclusion bodies in the locus ceruleus (LC) of mouse with protein bodies in the LC of human. Phosphotungstic acid-hematoxylin (PTAH) and the Mallory method were used as the anionic stains. The inclusion bodies and protein bodies, which stained with PTAH and the Mallory method in the brain, contain proteins that appear to belong to the same family. Although both inclusion and protein bodies were formed with the same arginine composition, their distribution in the brain was similar and similar physiological changes by morbidity were observed, and the fine structure of the inclusion bodies and protein bodies appeared to be different. The present findings suggest that mouse inclusion bodies and human protein bodies are different.
G. V. Russel, “The Nucleus Locus Coeruleus (Dorsolateralis Tegmenti),” Texas Reports on Biology & Medicine, Vol. 13, 1955, pp. 939-988.
Y. Katoh, M. Kanematsu and N. Shimizu, “Light-Microscopic Studies of Locus Coeruleus Region of the Mouse,” Brain and Nerve, Vol. 36, 1984, pp. 185-191.
D. G. Amaral and H. M. Sinnamon, “The Locus Coeruleus. Neurobiology of a Central Noradrenergic Nucleus,” Progress in Neurobiology, Vol. 9, 1977, pp. 147-196. http://dx.doi.org/10.1016/0301-0082(77)90016-8
Y. Katoh and N. Shimizu, “The Light and Electron-Microscopic Localization of Intracytoplasmic NucleolusLike Bodies in the Mouse Brain Stained by Holmes’ Silver Method,” Archivum Histologicum Japonicum, Vol. 45, 1982, pp. 325-333.
I. Kawabata, “Electron Microscopy of the Rat Hypothalamic Neurosecretory System. Il. Nucleolus-Like Inclusion Bodies in the Cytoplasm of Neurosecretory Cells,” Archivum Histologicum Japonicum, Vol. 26, 1965, pp. 101-113.
A. P. Anzil, H. Herrlinger and K. Blinzinger, “Nucleolus Inclusions in Neuronal Perikarya and Processes: Phase and Electron Microscope Observations on the Hypothalamus of the Mouse,” Zeitschrift für Zellforschung und Mikroskopische Anatomie, Vol. 146, No. 3, 1973, pp. 329-337. http://dx.doi.org/10.1007/BF02346225
M. A. Grillo, “Cytoplasmic Inclusions Resembling Nucleoli in Sympathetic neurons of Adult Rats,” The Journal of Cell Biology, Vol. 45, No. 1, 1970, pp. 100-117. http://dx.doi.org/10.1083/jcb.45.1.100
R. C. Santolaya, “Nucleolus-Like Bodies in the Neuronal Cytoplasm of the Mouse Arcuate Nucleus,” Zeitschrift für Zellforschung und Mikroskopische Anatomie, Vol. 146, No. 3, 1973, pp. 319-328. http://dx.doi.org/10.1007/BF02346224
C. Hindelang-Gertner, M. E. Stoeckel, A. Porte, H. D. Dellmann and B. Madarász, “Nematosomes or Nucleolus-Like Bodies in Hypothalamic Neurons, the Subfornical Organ and Adenohypophysial Cells of the Rat,” Cell and Tissue Research, Vol. 155, No. 2, 1974, pp. 211-219. http://dx.doi.org/10.1007/BF00221355
Y. Katoh, “The Nucleolus-Like Bodies in the Mouse Locus Coeruleus,” Brain and Nerve, Vol. 34, 1982, pp. 39-45.
Y. Katoh and N. Shimizu, “Identity of Holmes Positive Bodies with Electron Microscopically Demonstrable Nucleolus-Like Bodies in Neuronal Cytoplasm,” Stain Technology, Vol. 57, 1982, pp. 83-89.
W. Holmes, “Silver Staining of Nerve Axons in Paraffin Sections,” The Anatomical Record, Vol. 86, No. 2, 1943, pp. 157-187. http://dx.doi.org/10.1002/ar.1090860205
M. Bazelon, G. M. Fenichel and J. Randa II, “Studies on Neuromelanin. I. A Melanin System in the Human Adult Brain Stem,” Neurology, Vol. 17, 1967, pp. 512-519. http://dx.doi.org/10.1212/WNL.17.5.512
M. R. Issidorides, C. Mytilineou, W. O. Whetsel1 Jr. and M. D. Yahr, “Protein-Rich Cytoplasmic Bodies of Substantia Nigra and Locus Coeruleus. A Comparative Study in Parkinsonian and Normal Brain,” Archives of Neurology, Vol. 35, 1978, pp. 633-637. http://dx.doi.org/10.1001/archneur.1978.00500340009002
M. R. Issidorides and M. T. Panayotacopoulou, “Histochemical Protein-Markers of Monoamine Neurons,” Neuroscience Letters, Vol. 1, 1978, p. 270.
M. T. Panayotacopoulou and M. R. Issidorides, “Histochemical Protein-Markers of Monoamine Cell Bodies in Man,” Archives of Neurology, Vol. 39, 1982, pp. 635-639. http://dx.doi.org/10.1001/archneur.1982.00510220033007
J. Y. Terner, “Phosphotungstic Acid-Hematoxylin: Reactivity in Vitro,” Journal of Histochemistry & Cytochemistry, Vol. 14, No. 4, 1966, pp. 345-351. http://dx.doi.org/10.1177/14.4.345
D. M. Phillips and P. Simson, “Identification of Some Peptides from an Arginine-Rich Histone and Their Bearing on the Structure of Deoxyribonucleohistone,” Biochemical Journal, Vol. 82, 1962, pp. 236-241.
B. E. Magun and J. W. Kelly, “A New Fluorescent Method with Phenanthrenequinone for the Histochernical Demonstration of Arginine Residues in Tissues,” Journal of Histochemistry & Cytochemistry, Vol. 17, No. 12, 1969, pp. 821-827. http://dx.doi.org/10.1177/17.12.821
G. Quintarelli, M. Bellocci and R. Geremia, “On Phosphotungstic Acid Staining IV. Selectivity of the Staining Reaction,” Journal of Histochemistry & Cytochemistry, Vol. 21, No. 2, 1973, pp. 155-160. http://dx.doi.org/10.1177/21.2.155
M. R. Issidorides and G. D. Pappas, “Fine Structure of Neuronal Spherical Arginine-Rich Bodies of Substantia Nigra and Locus Coeruleus in the Human Brain,” Human Neurobiology, Vol. 6, 1988, pp. 239-246.
A. Yanai, R. Fujinaga, J. Kawano and K. Shinoda, “In Situ Hybridization for Electron Microscopy; with Special Reference to the Stigmoid Body,” Anatomical Science International, Vol. 79, 2004, p. 383.
Y. Y. Katoh, E. Yamazaki, K. Taniguti, K. Yamada and G. Isomura, “Light and Electron Microscopic Observation of Intracytoplasmic Inclusion Bodies in the Locus Coeruleus of the Hamster,” Archives of Histology and Cytology, Vol. 69, No. 2, 2006, pp. 129-134. http://dx.doi.org/10.1679/aohc.69.129
K. Hirosawa, “Electron Microscopic Studies on Pigment Granules in the Substantia Nigra and Locus Coeruleus of the Japanese Monkey (Macaca Fuscata Yakui),” Zeitschrift für Zellforschung und Mikroskopische Anatomie, Vol. 88, No. 2, 1968, pp. 187-203. http://dx.doi.org/10.1007/BF00703907
L. S. Forno and R. L. Norville, “Ultrastructural Study of the Human Locus Coeruleus (in Middle-Aged and Older Persons with and without Parkinsonism),” Proceedings of the VII International Congress on Neuropathology, Excerpta Medica and Akademia Lmiado, Amsterdam, 1975, pp. 459-462.
S. Sekiya, M. Tanaka, S. Hayashi and S. Oyanagi, “Light-and Electron-Microscopic Studies of Intracytoplasmic Acidophilic Granules in the Human Locus Coeruleus and Substantia Nigra,” Acta Neuropathologica, Vol. 56, No. 1, 1982, pp. 78-80. http://dx.doi.org/10.1007/BF00691186
H. H. Goebel, “Dense Core Mitochondria in Peripheral Ganglia of Man,” Acta Neuropathologica, Vol. 36, 1976, pp. 85-90. http://dx.doi.org/10.1007/BF00685151
Y. Y. Katoh, C. Kishi, H. Takano, K. Yamada and G. Isomura, “Localization of Nucleolus-Like Inclusion Bodies in the Neuronal Cytoplasm in Mouse Brains: A Study Using Holmes Modified Method,” Structure and Function, Vol. 1, 2003, pp. 3-11.
Y. Katoh and N. Shimizu, “Quantitative Changes of Holmes Positive Nucleolus-Like Inclusion Bodies in the Mouse Locus Coeruleus under Various Experimental Conditions,” Archivum Histologicum Japonicum, Vol. 46, No. 4, 1983, pp. 491-500. http://dx.doi.org/10.1679/aohc.46.491
M. R. Issidorides, “Cellular Factors in Manic Depressive Illness: Blood and Brain,” In: C. N. Stefanis, Ed., Recent Advances in Depression, Pergamon Press, London, 1983, pp. 99-107.
H. Ehringer and O. Hornykiewicz, “Beurteilung von Noradrenalin und Dopamin (3-hydroxytyramin) im Gehirn des Menschen und ihr Verhalten bei Erkrankungen des Extrapyramidalen Systems,” Klinische Wochenschrift, Vol. 38, 1960, pp. 1236-1239. http://dx.doi.org/10.1007/BF01485901
O. Hornykiewicz and S. J. Kish, “Biochemical Pathophysiology of Parkinson’s Disease,” Advances in Neurology, Vol. 45, 1987, pp. 19-34.
R. M. Post, E. K. Gordon, F. K. Goodwin and W. E. Bunney Jr., “Central Norepinephrine Metabolism in Affective Illness: MHPG in the Cerebrospinal Fluid,” Science, Vol. 179, 1973, pp. 1002-1003. http://dx.doi.org/10.1126/science.179.4077.1002