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Simultaneous Measurement of Neural Activities of Acute Mouse Hippocampal Slices Using Multi-Electrode Array System and Laser Confocal Calcium Imaging
Department of Biosciences, College of Humanities and Sciences, Nihon University, Tokyo, Japan
Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo, Japan
Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo, Japan
Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo, Japan
Department of Biosciences, College of Humanities and Sciences, Nihon University, Tokyo, Japan
- 1 Department of Biosciences, College of Humanities and Sciences, Nihon University, Tokyo, Japan
- 2 Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo, Japan
- 3 Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo, Japan
- 4 Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo, Japan
- 5 Department of Biosciences, College of Humanities and Sciences, Nihon University, Tokyo, Japan
Journal of Behavioral and Brain Science·Volume 07 (2017)·Pages 68–78·Published 6 February 2017·DOI10.4236/jbbs.2017.72008
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Abstract
Recently, non-invasive, real-time and multi-point measurement of neural activities has become possible by using a multi-electrode array (MEA). Another method for multi-point measurement is the fluorescent imaging technique using voltage indicator dyes or calcium indicator dyes. Especially, calcium imaging using fluorescent calcium indicator dyes is often more useful, because they exhibit larger changes in the fluorescence intensity than voltage indicator dyes and their fluorescence changes can be detect easily. Additionally, calcium signals play key roles in the brain function, such as the long-term potentiation (LTP) in the hippocampus, and calcium imaging can be a powerful tool to elucidate the brain function. In this study, we constructed a measurement apparatus combining the MEA system and laser confocal calcium imaging and simultaneously measured electric signals and calcium signals in acute mouse hippocampal slices. The obtained results showed the availability of the present method.
KeywordsMulti-Electrode ArrayLaser Confocal Calcium ImagingHippocampusAcute SliceLong-Term Potentiation
- Kandel, E.R. and Schwartz, J.H. (2013) Principles of Neural Science. 5th Edition, McGraw-Hill, New York, 1502.
- Lynch, G., Larsom, J., Keslo, S., Barrionuevo, G. and Shutter, F. (1983) Intracellular Injection of EGTA Block Induction of Hippocampal Long-Term Potentiation. Nature, 305, 719-721. https://doi.org/10.1038/305719a0
- Malenka, R.C., Kauer, J.A., Zucker, R. and Nicoll, R.A. (1988) Postsynaptic Calcium Is Sufficient for Potentiation of Hippocampal Synaptic Transmission. Science, 242, 81-84. https://doi.org/10.1126/science.2845577
- Regehr, W.G. and Tank, D.W. (1990) Postsynaptic NMDA Receptor-Mediated Calcium Accumulation in Hippocampal CA1 Pyramidal Cell Dendrite. Nature, 345, 807-810. https://doi.org/10.1038/345807a0
- Müller, W. and Conner, J.A. (1991) Dendritic Spines as Individual Neuronal Compartments for Synaptic Ca2+ Responses. Nature, 354, 73-76. https://doi.org/10.1038/354073a0
- Pine, J. (1980) Recording Action Potentials from Cultured Neurons with Extracellular Microcircuit Electrodes. Journal of Neuroscience Methods, 2, 19-31. https://doi.org/10.1016/0165-0270(80)90042-4
- Jimbo, Y., Tateno, T. and Robinson, H.P. (1999) Simultaneous Induction of Pathway-Specific Potentiation and Depression in Networks of Cortical Neurons. Biophysical Journal, 76, 670-678. https://doi.org/10.1016/S0006-3495(99)77234-6
- Oka, H., Sugimoto, K., Ogawa, R., Sugihara, H. and Taketani, M. (1999) A New Planar Multielectrode Array for Extracellular Recording: Application to Hippocampal Acute Slice. Journal of Neuroscience Methods, 93, 61-67. https://doi.org/10.1016/S0165-0270(99)00113-2
- Alhebshi, A.H., Gotoh, M. and Suzuki, I. (2013) Thymoquinone Protects Cultured Rat Primary Neurons against Amyloid Beta-Induced Neurotoxicity. Biochemical and Biophysical Research Communications, 433, 362-367. https://doi.org/10.1016/j.bbrc.2012.11.139
- Odaira, A., Saitoh, Y., Alhebshi, A.H., Gotoh, M. and Suzuki, I. (2014) Long-Term Electrophysiological Activity and Pharmacological Response of a Human Induced Pluripotent Stem Cell-Derived Neuron and Astrocyte Co-Culture. Biochemical and Biophysical Research Communications, 443, 1176-1181. https://doi.org/10.1016/j.bbrc.2013.12.142
- Ibi, D., Nitta, A., Ishige, K., Cen, X., Ohtakara, T., Nabeshima, T. and Ito, Y. (2010) Piccolo Knockdown-Induced Impairments of Spatial Learning and Long-Term Potentiation in the Hippocampal CA1 Region. Neurochemistry International, 56, 77-83. https://doi.org/10.1016/j.neuint.2009.09.004