An early unbalanced nutritional diet can induce affective disorders in adulthood. As well as stress in adolescence can accentuate these disorders. Both human and rat structural changes have been demonstrated in the hippocampus, likewise, oxidative stress may be involved in these disturbances. The objective of this study is to see the impact of a high-fructose diet (PN21) associated with chronic mild stress (CMS) at the end of adolescence (PN55) on metabolic and affective disorders in rats Wistar. This study was performed on four groups of male rats: control group, CMS for five weeks (PN55), fructose for ten weeks (PN21) and fructose for ten weeks (PN21) associated with CMS for five weeks (PN55). These animals underwent behavioral tests to evaluate their affective states (open field test, Sucrose preference test). After sacrifice, the dosage of glucose, triglycerides and total cholesterol was performed at the prefrontal cortex (CPF) and also at the hippocampus; the dosage of nitric oxide (NO) was performed, too. The bulk of our results show that fructose induces metabolic disturbances; the CMS induces a state of depression-like, while the association potentiated metabolic disturbances, depression-like state and also inducing anxiety. This study has shown that fructose and CMS can disrupt the various functions of the body and their association can potentiate these disturbances.
Wolf, A., Bray, G.A. and Popkin, B.M. (2008) A Short History of Beverages and How Our Body Treats Them. Obesity Reviews, 9, 151-164. https://doi.org/10.1111/j.1467-789X.2007.00389.x
Misra, A. and Khurana, L. (2009) Obesity and the Metabolic Syndrome in Developing Countries. Journal of Clinical Endocrinology & Metabolism, 93, 9-30. https://doi.org/10.1210/jc.2008-1595
Heinz, F., Lamprecht, W. and Kirsch, J. (1968) Enzymes of Fructose Metabolism in Human Liver. Journal of Clinical Investigation, 47, 1826-1832. https://doi.org/10.1172/JCI105872
Mayes, P.A. (1993) Intermediary Metabolism of Fructose. American Journal of Clinical Nutrition, 58, 754-765. https://doi.org/10.1093/ajcn/58.5.754S
Parks, E.J., Skokan, L.E., Timlin, M.T. and Dingfelder, C.S. (2008) Dietary Sugars Stimulate Fatty Acid Synthesis in Adults. Journal of Nutrition, 138, 1039-1046. https://doi.org/10.1093/jn/138.6.1039
Le KA, T.L. (2007) Metabolic Effects of Fructose. Curr Opin Clin Nutr Metabol Care., 10, 210-214.
Havel, P.J. (2005) Dietary Fructose: Implication for Dysregulation of Energy Homeostasis and Lipid/Carbohydrate Metabolism. Nutrition Reviews, 63, 133-137. https://doi.org/10.1111/j.1753-4887.2005.tb00132.x
Strekalova, T. and Steinbusch, H.W. (2010) Measuring Behavior in Mice with Chronicstress Depression Paradigm. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 34, 348–361. https://doi.org/10.1016/j.pnpbp.2009.12.014
Xue, X., Shao, S., Li, M., Shao, F. and Wang, W. (2013) Maternal Separation Induces Alterations of Serotonergic System in Different Aged Rats. Brain Research Bulletin, 95, 15-20. https://doi.org/10.1016/j.brainresbull.2013.03.003
Rouillon, F. (2008) Epidémiologie des troubles psychiatriques. Annales Médico-Psychologiques, revue psychiatrique, 166, 63-70. https://doi.org/10.1016/j.amp.2007.11.010
Zimmerman, M., McDermut, W. and Mattia, J.I. (2000) Frequency of Anxiety Disorders in Psychiatric Outpatients with Major Depressive Disorder. American Journal of Psychiatry, 157, 1337-1340. https://doi.org/10.1176/appi.ajp.157.8.1337
Lindqvist, A., Baelemans, A. and Erlanson-Albertsson, C. (2008) Effects of Sucrose, Glucose and Fructose on Peripheral and Central Appetite Signals. Regulatory Peptides, 150, 26-32. https://doi.org/10.1016/j.regpep.2008.06.008
Strekalova, T., Couch, Y., Kholod, N., Boyks, M., Malin, D., Leprince, P., et al. (2011) Update in the Methodology of the Chronic Stress Paradigm: Internal Control Matters. Behavioral and Brain Functions, 7, 1-18. https://doi.org/10.1186/1744-9081-7-9
Willner, P. (2005) Chronic Mild Stress (CMS) Revisited: Consistency and Behavioural Neurobiological Concordance in the Effects of CMS. Neuropsychobiology, 52, 90-110. https://doi.org/10.1159/000087097
Li, N., Liu, R.J., Dwyer, J.M., Banasr, M., Lee, B., Son, H., et al. (2011) Glutamate N-methyl-D-aspartate Receptor Antagonists Rapidly Reverse Behavioral and Synaptic Deficits Caused by Chronic Stress Exposure. Biological Psychiatry, 69, 754-761. https://doi.org/10.1016/j.biopsych.2010.12.015
Willner, P. (1997) The Chronic Mild Stress Procedure as an Animal Model of Depression: Valid, Reasonably Reliable, and Useful. Psychopharmacology, 134, 371-377. https://doi.org/10.1007/s002130050473
Archer, J. (1973) Tests for Emotionality in Rats and Mice: A Review. Animal Behaviour, 21, 205-235. https://doi.org/10.1016/S0003-3472(73)80065-X
Strekalova, T., Spanagel, R., Dolgov, O. and Bartsch, D. (2005) Stress-Induced Hyperlocomotion as a Confounding Factor in Anxiety and Depression Models in Mice. Behavioural Pharmacology, 16, 171-180. https://doi.org/10.1097/00008877-200505000-00006
Eagle, A.L., Mazei-Robison, M. and Robison, A.J. (2016) Sucrose Preference Test to Measure Stress-Induced Anhedonia. http://www.bio-protocol.org/e1822
Zhu, W.L., Shi, H.S., Wang, S.J., Wu, P., Ding, Z.B. and Lu, L. (2011) Hippocampal CA3 Calcineurin Activity Participates in Depressive-Like Behavior in Rats. Journal of Neurochemistry, 117, 1075-1086. https://doi.org/10.1111/j.1471-4159.2011.07285.x
Chiba, S., Numakawa, T., Ninomiya, M., Richards, M.C., Waka-bayashi, C. and Kunugi, H. (2012) Chronic Restraint Stress Causes Anxiety- and Depression-Like Behaviors, Downregulates Glucocorticoid Receptor Expression, and Attenuates Glutamate Release Induced by Brain-Derived Neurotrophic Factor in the Prefrontal Cortex. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 39, 112-119. https://doi.org/10.1016/j.pnpbp.2012.05.018
Eiland, L., Ramroop, J., Hill, M.N., Manley, J. and McEwen, B.S. (2012) Chronic Juvenile Stress Produces Corticolimbic Dendritic Architectural Remodeling and Modulates Emotional Behavior in Male and Female Rats. Psychoneuroendocrinology, 37, 39-47. https://doi.org/10.1016/j.psyneuen.2011.04.015
Matta, S.G., Linner, K.M. and Sharp, B.M. (1993) Interleukin-1 Alpha and Interleukin-1 Beta Stimulate Adrenocorticotropin Secretion in the Rat through a Similar Hypothalamic Receptor(s): Effects of Interleukin-1 Receptor Antagonist Protein. Neuroendocrinology, 57, 14-22. https://doi.org/10.1159/000126336
Korte, S.M. (2001) Corticosteroids in Relation to Fear, Anxiety and Psychopathology. Neuroscience and Biobehavioral Reviews, 25, 117-142. https://doi.org/10.1016/S0149-7634(01)00002-1
Dunn, A.J. and Swiergiel, A.H. (2008) The Role of Corticotropin-Releasing Factor and Noradrenaline in Stress-Related Responses, and the Inter-Relationships between the Two Systems. European Journal of Pharmacology, 583, 186-193. https://doi.org/10.1016/j.ejphar.2007.11.069
Nesher, E., Gross, M., Lisson, S., Tikhonov, T., Yadid, G. and Pinhasov, A. (2013) Differential Responses to Distinct Psychotropic Agents of Selectively Bred Dominant and Submissive Animals. Behavioural Brain Research, 236, 225-235. https://doi.org/10.1016/j.bbr.2012.08.040
Moussaieff, A., Gross, M., Nesher, E., Tikhonov, T., Yadid, G. and Pinhasov, A. (2012) Incensole Acetate Reduces Depressive-Like Behavior and Modulateshippocampal BDNF and CRF Expression of Submissive Animals. Journal of Psychopharmacology, 26, 1584-1593. https://doi.org/10.1177/0269881112458729
Wu, L.M., Han, H., Wang, Q.N., Hou, H.L., Tong, H., Yan, X.B. and Zhou, J.N. (2007) Mifepristone Repairs Region-Dependent Alteration of Synapsin I in Hippocampus in Rat Model of Depression. Neuropsychopharmacology, 32, 2500-2510.
Kvarta, M.D., Bradbrook, K.E., Dantrassy, H.M., Bailey, A.M. and Thompson, S.M. (2015) Corticosterone Mediates the Synaptic and Behavioral Effects of Chronic Stress at Rat Hippocampal Temporoammonic Synapses. Journal of Neurophysiology, 114, 1713-1724.
Goshen, I., Kreisel, T., Ben-Menachem-Zidon, O., Licht, T., Weidenfeld, J., Ben-Hur, T. and Yirmiya, R. (2008) Brain Interleukin-1 Mediates Chronic Stress-Induced Depression in Mice via Adrenocortical Activation and Hippocampal Neurogenesis Suppression. Molecular Psychiatry, 13, 717-728.
Chen, J., Wang, Z.Z., Zuo, W., Zhang, S., Chu, S.F. and Chen, N.H. (2016) Effects of Chronic Mild Stress on Behavioral and Neurobiological Parameters—Role of Glucocorticoid. Hormones and Behavior, 78, 150-159.
Chang, C.H. and Grace, A.A. (2014) Amygdala-Ventral Pallidum Pathway Decreases Dopamine Activity after Chronic Mild Stress in Rats. Biological Psychiatry, 76, 223-230.
Willner, P. (2016) Reliability of the Chronic Mild Stress Model of Depression: A User Survey. Neurobiology of Stress, 6, 68-77.
Hunter, R.G., McCarthy, K.J., Milne, T.A., Pfaff, D.W. and McEwen, B.S. (2009) Regulation of Hippocampal H3 Histone Methylation by Acute and Chronic Stress. Proceedings of the National Academy of Sciences, 106, 20912-20917. https://doi.org/10.1073/pnas.0911143106
Dannlowski, U., et al. (2008) 5-HTTLPR Biases Amygdala Activity in Response to Masked Facial Expressions in Major Depression. Neuropsychopharmacology, 33, 418-424. https://doi.org/10.1038/sj.npp.1301411
Wei, Q., et al. (2004) Glucocorticoid Receptor Overexpression in Forebrain: A Mouse Model of Increased Emotional Lability. Proceedings of the National Academy of Sciences, 101, 11851-11856. https://doi.org/10.1073/pnas.0402208101
Filho, C.B., Jesse, C.R., Donato, F., Giacomeli, R., Del Fabbro, L., da Silva Antunes, M., de Gomes, M.G., Goes, A.T., Boeira, S.P., Prigol, M. and Souza, L.C. (2015) Chronic Unpredictable Mild Stress Decreases BDNF and NGF Levels and Na(t), K(t)-ATPase Activity in the Hippocampus and Prefrontal Cortex of Mice: Antidepressant Effect of Chrysin. Neuroscience, 289, 367-380.
Yolanda, B., Lombardo, A.E., Gustavo Hein, A.E. and Adriana, C. (2006) Metabolic Syndrome: Effects of n-3 PUFAs on a Model of Dyslipidemia, Insulin Resistance and Adiposity. Lipids, 42, 427-437.
Robbez-Masson, V., Lucas, A., Gueugneau, A.M., Macaire, J.P., Paul, J.P., Grynberg, A. and Rousseau, D. (2008) Long-Chain (n-3) Polyunsaturated Fatty Acids Prevent Metabolic and Vascular Disorders in Fructose-Fed Rats. The Journal of Nutrition, 138, 1915-1922. https://doi.org/10.1093/jn/138.10.1915
Catalina-Romero, C., Calvo, E., Sanchez-Chaparro, M.A., Valdivielso, P., Sainz, J.C., Cabrera, M., Gonzalez-Quintela, A. and Roman, J. (2013) The Relationship between Job Stress and Dyslipidemia. Scandinavian Journal of Public Health, 41, 142-149. https://doi.org/10.1177/1403494812470400
Saad, A., Virella, G., Chassereau, Ch., et al. (2006) OxLDL Immune Complexes Activate Complement and Induce Cytokine Production by MonoMac 6 Cells and Human Macrophages. The Journal of Lipid Research, 47, 1975-1983. https://doi.org/10.1194/jlr.M600064-JLR200
Barone, E., Di Domenico, F., Cassano, T., Arena, A., Tramutola, A., Lavecchia, M.A., Coccia, R., Butterfield, D.A. and Perluigi, M. (2016) Impairment of Biliverdin Reductase—A Promotes Brain Insulin Resistance in Alzheimer Disease: A New Paradigm. Free Radical Biology & Medicine, 91, 127-142. https://doi.org/10.1016/j.freeradbiomed.2015.12.012
De Sousa Rodrigues, M.E., Bekhbat, M., Houser, M.C., Chang, J., Walker, D.I., Jones, D.P., Oller do Nascimento, C.M.P., Barnum, C.J. and Tansey, M.G. (2016) Chronic Psychological Stress and High-Fat Highfructose Diet Disrupt Metabolic and Inflammatory Gene Networks in the Brain, Liver, and Gut and Promote Behavioral Deficits in Mice. Brain, Behavior, and Immunity, 59, 158-172.