Although hydroxyapatite is commonly used as a scaffold for bone regeneration, sponges may be suitable because of the adaptability to the defect. To use as a scaffold, the fiber of sponge would be coated with any adhesive to storage stem cells in the sponges. Fiber in the structure of commercially available sponges was coated by immersion in dextran solution and air dried. After seeding of rat bone marrow cells (rBMCs), the sponges were implanted subcutis of rats for estimate osteogenesis in vivo. The level of osteocalcin was 25.28 ± 5.71 ng/scaffold and that of Ca was 129.20 ± 19.69 μg/scaffold. These values were significantly high- er than those in sponges without dextran coating (p < 0.01). It was thought that rBMCs could be stored on the shelf by dextran deposition in the fiber of the sponge. In vivo examination, dextran induced osteogenesis by rBMCs in many spaces in the inner structure of the sponge.
Yamada, Y., Ueda, M., Naiki, T. and Nagasaka, T. (2004) Tissue-engineered injectable bone regeneration for osseointegrated dental implants. Clinical Oral Implants Research, 15(5), 589-597.
Ohazama, A., Modino, S.A., Miletich, I. and Sharpe, P.T. (2004) Stem-cell-based tissue engineering of murine teeth. Journal of Dental Research, 83(7), 518-522.
Hu, B., Unda, F., Bopp-Kuchler, S., Jimenez, L., Wang, X.J., Ha?kel, Y., Wang, S.L. and Lesot, H. (2006) Bone marrow cells can give rise to ameloblast-like cells. Journal of Dental Research, 85(5), 416-421.
Yen, A.H. and Sharpe, P.T. (2008) Stem cells and tooth tissue engineering. Cell Tissue Research, 331(1), 359-372.
Jing, W., Wu, L., Lin, Y., Liu, L., Tang, W. and Tian, W. (2008) Odontogenic differentiation of adipose-derived stem cells for tooth regeneration: Necessity, possibility, and strategy. Medical Hypotheses, 70(3), 540-542.
Duailibi, M.T., Duailibi, S.E., Young, C.S., Bartlett, J.D., Vacanti, J.P. and Yelick, P.C. (2004) Bioengineered teeth from cultured rat tooth bud cells. Journal of Dental Research, 83(7), 523-528.
Zhang, W., Walboomers, X.F., Van Kuppevelt, T.H., Daamen, W.F., Van Damme, P.A. and Bian, Z., (2008) In vivo evaluation of human dental pulp stem cells differentiated towards multiple lineages. Journal of Tissue Engineering and Regenerative Medicine, 2(2-3), 117-125.
Satoyoshi, M., Koizumi, T., Teranaka, T., Iwamoto, T., Takita, H., Kuboki, Y., Saito, S. and Mikuni-Takagaki, Y. (1995) Extracellular processing of dentin matrix protein in the mineralizing odontoblast culture. Calcified Tissue International, 57(3), 237-241.
Papagerakis, P., Berdal, A., Mesbah, M., Peuchmaur, M., Malaval, L., Nydegger, J., Simmer, J. and Macdougall, M. (2002) Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth. Bone, 30(2), 377-385.
Jeong, W.K., Oh, S.H., Lee, J.H. and Im, G.I. (2008) Repair of osteochondral defects with a construct of mesenchymal stem cells and a polydioxanone/poly (vinyl alcohol) scaffold. Biotechnology and Applied Biochemistry, 49(2), 155-164.
Robinson, B.P., Hollinger, J.O., Szachowicz, E.H. and Brekke, J. (1995) Calvarial bone repair with porous D, L-polylactide. Otolaryngology-Head and Neck Surgery, 112(6), 707-713.
Chang, Y.S., Oka, M., Kobayashi, M., Gu, H.O., Li, Z.L., Nakamura, T. and Ikada, Y. (1996) Significance of interstitial bone ingrowth under load-bearing conditions: A comparison between solid and porous implant materials. Biomaterials, 17(11), 1141-1148.
Nuttelman, C.R., Mortisen, D.J., Henry, S.M. and Anseth, K.S. (2001) Attachment of fibronectin to poly (vinyl alcohol) hydrogels promotes NIH3T3 cell adhesion, proliferation, and migration. Journal of Biomedical Materials Research, 57(2), 217-223.
Cadée, J.A., van Luyn, M.J., Brouwer, L.A., Plantinga, J.A., van Wachem, P.B., de Groot, C.J., den Otter, W. and Hennink, W.E. (2000) In vivo biocompatibility of dextran-based hydrogels. Journal of Biomedical Materials Research, 50(3), 397-404.
Liu, J.M., Haroun-Bouhedja, F. and Boisson-Vidal, C. (2000) Analysis of the in vitro inhibition of mammary adenocarcinoma cell adhesion by sulfated polysaccharides. Anticancer Research, 20(5A), 3265-3271.
Maniatopoulos, C., Sodek, J. and Melcher, A.H. (1988) Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell and Tissue Research, 254(2), 317-330.
Ohgushi, H., Dohi, Y., Katuda, T., Tamai, S., Tabata, S. and Suwa, Y. (1996) In vitro bone formation by rat marrow cell culture. Journal of Biomedical Materials Research, 32(3), 333-340.
Lemus, D. (1995) Contributions of hetero specific tissue recombinations to odontogenesis. International Journal of Developmental Biology, 39(1), 291-297.
Lewin-Epstein, J., Azaz, B. and Ulmansky, M. (1969) Fate of osteogenic tissue transferred to the subcutaneous area by means of polyvinyl-formal sponge. Israel Journal of Medical Sciences, 5(2), 365-372.
Tsuruga, E., Takita, H., Itoh, H., Wakisaka, Y. and Kuboki, Y. (1997) Pore size of porous hydroxyapatite as the cell-substratum controls BMP-induced osteogenesis. The Journal of Biochemistry, 121(2), 317-324.
Zajaczkowski, M.B., Cukierman, E., Galbraith, C.G. and Yamada, K.M. (2003) Cell-matrix adhesions on poly (vinyl alcohol) hydrogels. Tissue Engineering, 9(3), 525-533.
Tsuji, Y., Yoshimura, N., Aoki, H., Sharov, A.A., Ko, M.S., Motohashi, T. and Kunisada, T. (2008) Maintenance of undifferentiated mouse embryonic stem cells in suspension by the serum- and feeder-free defined culture condition. Developmental Dynamics, 237(8), 2129-2138.
Wang, Y.W., Wu, Q. and Chen, G.Q. (2003) Reduced mouse fibroblast cell growth by increased hydrophilicity of microbial polyhydroxyalkanoates via hyaluronan coating. Biomaterials, 24(25), 4621-4629.
Li, D., Dai, K. and Tang, T. (2008) Effects of dextran on proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells. Cytotherapy, 10(6), 587-596.
Cascone, M.G., Maltinti, S., Barbani, N. and Laus, M. (1999) Effect of chitosan and dextran on the properties of poly (vinyl alcohol) hydrogels. Journal of Materials Science: Materials in Medicine, 10(7), 431-435.
Thébaud, N.-B., Pierron, D., Bareille, R., Le Visage, C., Letourneur, D. and Bordenave, L. (2007) Human endothelial progenitor cell attachment to polysaccharide- based hydrogels: A pre-requisite for vascular tissue engineering. Journal of Materials Science: Materials in Medicine, 18(2), 339-345.
Noble, B.S., Dean, V., Loveridge, N. and Thomson, B.M. (1995) Dextran sulfate promotes the rapid aggregation of porcine bone-marrow stromal cells. Bone, 17(4), 375-382.
Lévesque, S.G., Lim, R.M. and Shoichet, M.S. (2005) Macroporous interconnected dextran scaffolds of controlled porosity for tissue-engineering applications. Biomaterials, 26(35), 7436-7446.