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In vitro differentiation of human umbilical cord-derived mesenchymal stem cells into CD34+ cells via CD34 antibody

¹ Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China Tuhua Bioengineering Company Ltd., Siping, China
² Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China
³ Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China Tuhua Bioengineering Company Ltd., Siping, China
⁴ Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China Tuhua Bioengineering Company Ltd., Siping, China
⁵ Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China Tuhua Bioengineering Company Ltd., Siping, China
⁶ Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China

Journal of Biomedical Science and Engineering·Volume 06 (2013)·Pages 53–58·Published 6 August 2013·DOI10.4236/jbise.2013.68A1005

Abstract

CD34+ cells differentiated from mesenchymal stem cells (MSCs) have a strong biological function in cardiovascular regeneration. However, the molecular mechanisms of and the methods to improve the CD34+ cell differentiation from MSCs, especially from human MSCs (hUC-MSCs) are still unclear. In the current study, the effect of CD34 antibody on the CD34+ cell differentiation from human umbilical cord (UC)-derived MSCs (hUC-MSCs) is determined. The results have demonstrated that the expression of cd34 protein is significantly increased in hUC-MSCs treated with CD34 antibody. In addition, the cell proliferation is increased in hUC-MSCs after treatment with CD34 antibody. Moreover, the expression of PI3K, AKT, p-AKT proteins, which are signaling molecules related to stem cell differentiation, is increased by CD34 antibody. The results suggest that CD34 antibody could promote the differentiation of hUC-MSCs into CD34+ cells and PI3K/AKT may be involved in this important process.

KeywordsDifferentiationHuman Umbilical Cord-Derived Mesenchymal Stem CellsCD34&ltsup&gt+&lt/sup&gtCells

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<i>In vitro</i> differentiation of human umbilical cord-derived mesenchymal stem cells into CD34<sup>+</sup> cells via CD34 antibody

Abstract

References (18)