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Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids
Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Sakuragaoka, Kagoshima, Japan
Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Kagoshima, Japan
Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Gakkocho-dori, Chuo-ku, Niigata, Japan
Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Kagoshima, Japan
- 1 Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Sakuragaoka, Kagoshima, Japan
- 2 Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Kagoshima, Japan
- 3 Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Gakkocho-dori, Chuo-ku, Niigata, Japan
- 4 Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Kagoshima, Japan
Journal of Biomedical Science and Engineering·Volume 07 (2014)·Pages 105–107·Published 20 February 2014·DOI10.4236/jbise.2014.73013
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Abstract
Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli ( E. coli ) in liquid medium, brief centrifugation for precipitation of bacteri al pellets, and subsequent lysis of the pellets. This pro cess is time-consuming and laborious, especially when the sample number is high. Here, we describe a more convenient method for MiniPrep analysis that utilizes solid medium-based cultivation of bacteria.
KeywordsAgar Plate<i>E. coli</i>MiniPrep AnalysisGene EngineeringPlasmid
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