Differentiation of Menstrual Blood Derived Stem Cell (MensSCs) to Hepatocyte-Liked Cell on Three Dimensional Nanofiberscaffold: Poly Caprolacton (PCL) — Oak Academic Publishing
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Differentiation of Menstrual Blood Derived Stem Cell (MensSCs) to Hepatocyte-Liked Cell on Three Dimensional Nanofiberscaffold: Poly Caprolacton (PCL)
Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
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Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
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Departments of Embryology and Stem Cells, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
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Department of Biochemistry, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
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Anatomy, Histomorphometry and Stereology Research Center, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
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Departments of Embryology and Stem Cells, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
,
Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
,
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
,
Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
,
Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
,
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
,
Departments of Embryology and Stem Cells, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
1 Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
2 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
3 Departments of Embryology and Stem Cells, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
4 Department of Biochemistry, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
5 Anatomy, Histomorphometry and Stereology Research Center, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
6 Departments of Embryology and Stem Cells, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
7 Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
8 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
9 Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
10 Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
11 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
12 Departments of Embryology and Stem Cells, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Menstrual blood stem cells (MensSCs) have enormous potential as a source for cell replacement therapies. Since there is a major concern in utilization of nanofibers in tissue engineering of stem cells, we examined the potential of MensSCs to differentiate into hepatocytes, using different protocols and compare cells, with two-dimensional (2D) and three-dimensional (3D) culture systems. Cell characterization experiments of MensSCs have demonstrated that they are multipotent stem cells similar to mesenchymal stem cells, which can successfully differentiate into osteogenic and adipogenic lineages. The efficiency of the cells on the scaffold was appraised by scanning electron microscopy (SEM), MTT assay, and hematoxylin and eosin (H&E) staining. Thereafter, the differentiation protocols were developed by hepatocyte growth factor (HGF) and oncostatin M (OSM) with serum-supplemented or serum-free culture media up to 30 days. Immunofluorescence analysis and ELISA assay revealed the expression of albumin (ALB) in differentiated cells. Hepatocyte-like cells expressed liver-specific gene such as albumin(ALB), α -fetoprotein (AFP), tyrosine aminotransferase (TAT) and cytochrome P450 subunit 7a1 (Cyp7a1) at mRNA levels. In conclusion, the evidences presented in this study show that the nanofiber scaffold and MensSCs may provide a source of differentiated cells for treatment of liver diseases.
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