Potential Antimicrobial Effects of Gatifloxacin on Periodontopathic Bacteria <i>in Vitro</i>
- 1 Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan
- 2 Department of Pharmacology, Tokyo Dental College, Tokyo, Japan
- 3 Department of Pharmacology, Tokyo Dental College, Tokyo, Japan
- 4 Department of Oral and Maxillofacial Implantology, Tokyo Dental College, Tokyo, Japan
- 5 Department of Liberal Arts, College of Bioresource Sciences, Nihon University, Kanagawa, Japan
- 6 Department of Removable Partial Prosthodontics, Tokyo Dental College, Tokyo, Japan
- 7 Department of Pharmacology, Tokyo Dental College, Tokyo, Japan
- 8 Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan
Abstract
The aim of this study was to identify the potential antibacterial effects of gatifloxacin on periodontal pathogens including Aggregatibacter actinomycetemcomitans , Porphyromonas gingi-valis , and Prevotella intermedia . The minimum inhibitory concentrations (MIC) of gatifloxacin and its bactericidal effects were investigated. Gatifloxacin inhibited the growth of all three kinds of periodontopathic bacteria tested in broth. The MIC value of 2.5 nM was found to be the most effective in inhibiting A. actinomycetemcomitans . An adenosine triphosphate biolumi-nescence assay revealed that gatifloxacin exhibited bactericidal effects on the tested bacteria in a time-dependent manner. The safety of gatifloxacin in mammalian cells was evaluated by assessing the viability of normal human dermal fibroblast (NHDF) cells treated with gatifloxacin. Almost all NHDF cells survived after 2-d culture, while 81% of the cells survived after 4-d culture when treated with 1.0 × 10 3 nM gatifloxacin. These results indicate that gatifloxacin is a possible drug for local administration to prevent periodontal infection.
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