Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts — Oak Academic Publishing
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Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts
Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
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Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
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Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
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Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
1 Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
2 Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
3 Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
4 Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, China
The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E 2 ) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E 2 , PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.
KeywordsPEMFsCo-CulturedRANK mRNANFATc1 mRNA
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